Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia
Staphylococcus aureus is recognized for causing contagious mastitis in cattle. Elimination of the organism in affected animals is challenging due to its potential antibiotic resistance mechanisms and virulence factors. The aim of the study was the pheno- and genotypic epidemiological characterizatio...
| Main Authors: | , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/21541 https://www.sciencedirect.com/science/article/pii/S0958694624002085 https://doi.org/10.1016/j.idairyj.2024.106088 |
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| author | Ágredo-Campos, Ángela Sofía Camussone, Cecilia Akineden, Ömer Fernández-Silva, Jorge A. Ramírez-Vásquez, Nicolás F. |
| author_browse | Akineden, Ömer Camussone, Cecilia Fernández-Silva, Jorge A. Ramírez-Vásquez, Nicolás F. Ágredo-Campos, Ángela Sofía |
| author_facet | Ágredo-Campos, Ángela Sofía Camussone, Cecilia Akineden, Ömer Fernández-Silva, Jorge A. Ramírez-Vásquez, Nicolás F. |
| author_sort | Ágredo-Campos, Ángela Sofía |
| collection | INTA Digital |
| description | Staphylococcus aureus is recognized for causing contagious mastitis in cattle. Elimination of the organism in affected animals is challenging due to its potential antibiotic resistance mechanisms and virulence factors. The aim of the study was the pheno- and genotypic epidemiological characterization of S. aureus isolated from bulk tank milk samples obtained from three municipalities in the northern region of Antioquia. Twenty-one S. aureus isolates from 150 bulk tank milk samples were characterized by evaluating antimicrobial susceptibility to 17 antibiotics using the VITEK 2, and identifying several genes encoding for virulence factors, enterotoxins, and the agr groups by conventional PCR. The clonal relationship between the isolates was assessed using macrorestriction fragment analysis (MRFA) of chromosomal DNA by pulsed-field gel electrophoresis (PFGE). Most of the isolates showed susceptibility to the tested antibiotics; 19% exhibited resistance to tetracycline and all isolates showed beta-lactamases. Molecular typing revealed that 76% carried the agrI and cap5 genes. Seven isolates harbored genes coding for staphylococcal enterotoxins (SE), with seh being the most identified gene. Furthermore, MRFA demonstrated high heterogeneity among the isolates, resulting in the assignment of 18 different MRFA patterns. These results indicate phenotypic resistance to tetracycline and beta-lactams, the presence of genes encoding for staphylococcal enterotoxins, and high heterogeneity among S. aureus isolates from bulk tank milk. |
| format | info:ar-repo/semantics/artículo |
| id | INTA21541 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA215412025-03-05T14:04:40Z Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia Ágredo-Campos, Ángela Sofía Camussone, Cecilia Akineden, Ömer Fernández-Silva, Jorge A. Ramírez-Vásquez, Nicolás F. Staphylococcus aureus Epidemiology Milk Phenology Bovine Mastitis Epidemiología Leche Fenología Mastítis Bovina Colombia Staphylococcus aureus is recognized for causing contagious mastitis in cattle. Elimination of the organism in affected animals is challenging due to its potential antibiotic resistance mechanisms and virulence factors. The aim of the study was the pheno- and genotypic epidemiological characterization of S. aureus isolated from bulk tank milk samples obtained from three municipalities in the northern region of Antioquia. Twenty-one S. aureus isolates from 150 bulk tank milk samples were characterized by evaluating antimicrobial susceptibility to 17 antibiotics using the VITEK 2, and identifying several genes encoding for virulence factors, enterotoxins, and the agr groups by conventional PCR. The clonal relationship between the isolates was assessed using macrorestriction fragment analysis (MRFA) of chromosomal DNA by pulsed-field gel electrophoresis (PFGE). Most of the isolates showed susceptibility to the tested antibiotics; 19% exhibited resistance to tetracycline and all isolates showed beta-lactamases. Molecular typing revealed that 76% carried the agrI and cap5 genes. Seven isolates harbored genes coding for staphylococcal enterotoxins (SE), with seh being the most identified gene. Furthermore, MRFA demonstrated high heterogeneity among the isolates, resulting in the assignment of 18 different MRFA patterns. These results indicate phenotypic resistance to tetracycline and beta-lactams, the presence of genes encoding for staphylococcal enterotoxins, and high heterogeneity among S. aureus isolates from bulk tank milk. EEA Rafaela Fil: Ágredo-Campos, Ángela Sofía. Universidad de Antioquia. Facultad de Ciencias Agrarias. Escuela de Medicina Veterinaria. Grupo Centauro; Colombia Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Camussone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Akineden, Ömer. Justus-Liebig-University Giessen. Institute of Veterinary Food Science. Dairy Sciences; Alemania Fil: Fernández-Silva, Jorge A. Universidad de Antioquia. Facultad de Ciencias Agrarias. Escuela de Medicina Veterinaria. Grupo Centauro; Colombia Fil: Ramírez-Vásquez, Nicolás F. Universidad de Antioquia. Facultad de Ciencias Agrarias. Escuela de Medicina Veterinaria. Grupo Centauro; Colombia 2025-03-05T13:55:50Z 2025-03-05T13:55:50Z 2025-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21541 https://www.sciencedirect.com/science/article/pii/S0958694624002085 0958-6946 1879-0143 https://doi.org/10.1016/j.idairyj.2024.106088 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier International Dairy Journal 160 : 106088. (January 2025) |
| spellingShingle | Staphylococcus aureus Epidemiology Milk Phenology Bovine Mastitis Epidemiología Leche Fenología Mastítis Bovina Colombia Ágredo-Campos, Ángela Sofía Camussone, Cecilia Akineden, Ömer Fernández-Silva, Jorge A. Ramírez-Vásquez, Nicolás F. Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia |
| title | Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia |
| title_full | Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia |
| title_fullStr | Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia |
| title_full_unstemmed | Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia |
| title_short | Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia |
| title_sort | pheno and genotypic epidemiological characterization of staphylococcus aureus isolated from bulk tank milk in colombia |
| topic | Staphylococcus aureus Epidemiology Milk Phenology Bovine Mastitis Epidemiología Leche Fenología Mastítis Bovina Colombia |
| url | http://hdl.handle.net/20.500.12123/21541 https://www.sciencedirect.com/science/article/pii/S0958694624002085 https://doi.org/10.1016/j.idairyj.2024.106088 |
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