Enhancing Organic Selenium Forms in Alfalfa Forage Through Inorganic Selenium Foliar Application: Insights from Laboratory and Field Studies Using X-Ray Spectroscopy
Selenium (Se) is an essential micronutrient, yet its deficiency remains a global concern. This study investigates the biofortification of alfalfa (Medicago sativa cv. ProINTA Super Monarca GR9) via foliar Se application to enhance Se accumulation and transformation into bioavailable organic forms. A...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/21517 https://www.mdpi.com/2073-4395/15/3/580 https://doi.org/10.3390/agronomy15030580 |
| _version_ | 1855038414170095616 |
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| author | Sánchez-Martín, María Jesús Gaggiotti, Monica Del Carmen Simonelli, Laura Marini, Carlo Marini, Federico Boada, Roberto Llugany, Mercè Valiente, Manuel Céccoli, Gabriel Stoffel, María Micaela Viltres-Portales, Marcia Picco, Juan Manuel Guevara, María Gabriela Muñoz, Fernando Felipe |
| author_browse | Boada, Roberto Céccoli, Gabriel Gaggiotti, Monica Del Carmen Guevara, María Gabriela Llugany, Mercè Marini, Carlo Marini, Federico Muñoz, Fernando Felipe Picco, Juan Manuel Simonelli, Laura Stoffel, María Micaela Sánchez-Martín, María Jesús Valiente, Manuel Viltres-Portales, Marcia |
| author_facet | Sánchez-Martín, María Jesús Gaggiotti, Monica Del Carmen Simonelli, Laura Marini, Carlo Marini, Federico Boada, Roberto Llugany, Mercè Valiente, Manuel Céccoli, Gabriel Stoffel, María Micaela Viltres-Portales, Marcia Picco, Juan Manuel Guevara, María Gabriela Muñoz, Fernando Felipe |
| author_sort | Sánchez-Martín, María Jesús |
| collection | INTA Digital |
| description | Selenium (Se) is an essential micronutrient, yet its deficiency remains a global concern. This study investigates the biofortification of alfalfa (Medicago sativa cv. ProINTA Super Monarca GR9) via foliar Se application to enhance Se accumulation and transformation into bioavailable organic forms. A controlled environment experiment in a plant growth chamber and a one-season open-field trial (January 2023, Argentina) were conducted. Treatments included sodium selenate (Se(VI)), sodium selenite (Se(IV)), and a 1:1 mixture, applied at 45 and 90 g Se ha−1, with and without the biostimulant BIOFORGE®. Treated plants exhibited increased Se content, correlating with the applied doses. X-ray absorption spectroscopy (XAS) confirmed that most inorganic Se was transformed into organic Se forms, with Se(IV) treatments yielding the highest concentrations of organic Se species such as selenocysteine (SeCys) and selenomethionine (SeMet). Open-field trials showed a complete conversion of Se, though total Se accumulation was lower than in controlled conditions. Se treatments did not affect forage quality or biomass production. The biostimulant slightly reduced Se uptake but did not compromise biofortification. These results highlight Se(IV) as the optimal treatment for alfalfa biofortification, presenting a sustainable strategy to enhance dietary Se intake through functional foods. |
| format | info:ar-repo/semantics/artículo |
| id | INTA21517 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA215172025-02-28T13:52:18Z Enhancing Organic Selenium Forms in Alfalfa Forage Through Inorganic Selenium Foliar Application: Insights from Laboratory and Field Studies Using X-Ray Spectroscopy Sánchez-Martín, María Jesús Gaggiotti, Monica Del Carmen Simonelli, Laura Marini, Carlo Marini, Federico Boada, Roberto Llugany, Mercè Valiente, Manuel Céccoli, Gabriel Stoffel, María Micaela Viltres-Portales, Marcia Picco, Juan Manuel Guevara, María Gabriela Muñoz, Fernando Felipe Selenio Experimentación en Laboratorio Experimentación en Campo Espectros Copia de Rayos X Espectroscopia Selenium Medicago sativa Laboratory Experimentation Field Experimentation X-ray Spectroscopy Spectroscopy Alfalfa Lucerne Selenium (Se) is an essential micronutrient, yet its deficiency remains a global concern. This study investigates the biofortification of alfalfa (Medicago sativa cv. ProINTA Super Monarca GR9) via foliar Se application to enhance Se accumulation and transformation into bioavailable organic forms. A controlled environment experiment in a plant growth chamber and a one-season open-field trial (January 2023, Argentina) were conducted. Treatments included sodium selenate (Se(VI)), sodium selenite (Se(IV)), and a 1:1 mixture, applied at 45 and 90 g Se ha−1, with and without the biostimulant BIOFORGE®. Treated plants exhibited increased Se content, correlating with the applied doses. X-ray absorption spectroscopy (XAS) confirmed that most inorganic Se was transformed into organic Se forms, with Se(IV) treatments yielding the highest concentrations of organic Se species such as selenocysteine (SeCys) and selenomethionine (SeMet). Open-field trials showed a complete conversion of Se, though total Se accumulation was lower than in controlled conditions. Se treatments did not affect forage quality or biomass production. The biostimulant slightly reduced Se uptake but did not compromise biofortification. These results highlight Se(IV) as the optimal treatment for alfalfa biofortification, presenting a sustainable strategy to enhance dietary Se intake through functional foods. EEA Rafaela Fil: Sánchez-Martín, María Jesús. Universitat Autònoma de Barcelona. Faculty of Science. Department of Chemistry. GTS Research Group; España Fil: Gaggiotti, Monica Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Gaggiotti, Monica Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Simonelli, Laura. ALBA Synchrotron; España Fil: Marini, Carlo. ALBA Synchrotron; España Fil: Marini, Federico. Sapienza University of Rome. Department of Chemistry; Italia Fil: Boada, Roberto. Universitat Autònoma de Barcelona. Faculty of Science. Department of Chemistry. GTS Research Group; España Fil: Llugany, Mercè. Universitat Autonòma de Barcelona. Faculty of Biosciences. Plant Physiology Group (BABVE); España Fil: Valiente, Manuel. Universitat Autònoma de Barcelona. Faculty of Science. Department of Chemistry. GTS Research Group; España Fil: Céccoli, Gabriel. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Cátedra de Fisiología Vegetal; Argentina Fil: Céccoli, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro); Argentina Fil: Céccoli, Gabriel. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro); Argentina Fil: Stoffel, María Micaela. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Cátedra de Fisiología Vegetal; Argentina Fil: Viltres-Portales, Marcia. Universitat Autònoma de Barcelona. Faculty of Science. Department of Chemistry. GTS Research Group; España Fil: Picco, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Guevara, María Gabriela. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Biológicas (IIB); Argentina Fil: Guevara, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas (IIB); Argentina Fil: Muñoz, Fernando Felipe. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Cátedra de Fisiología Vegetal; Argentina Fil: Muñoz, Fernando Felipe. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Biológicas (IIB); Argentina Fil: Muñoz, Fernando Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas (IIB); Argentina 2025-02-28T13:48:58Z 2025-02-28T13:48:58Z 2025-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21517 https://www.mdpi.com/2073-4395/15/3/580 2073-4395 https://doi.org/10.3390/agronomy15030580 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Agronomy 15 (3) : 580. (February 2025) |
| spellingShingle | Selenio Experimentación en Laboratorio Experimentación en Campo Espectros Copia de Rayos X Espectroscopia Selenium Medicago sativa Laboratory Experimentation Field Experimentation X-ray Spectroscopy Spectroscopy Alfalfa Lucerne Sánchez-Martín, María Jesús Gaggiotti, Monica Del Carmen Simonelli, Laura Marini, Carlo Marini, Federico Boada, Roberto Llugany, Mercè Valiente, Manuel Céccoli, Gabriel Stoffel, María Micaela Viltres-Portales, Marcia Picco, Juan Manuel Guevara, María Gabriela Muñoz, Fernando Felipe Enhancing Organic Selenium Forms in Alfalfa Forage Through Inorganic Selenium Foliar Application: Insights from Laboratory and Field Studies Using X-Ray Spectroscopy |
| title | Enhancing Organic Selenium Forms in Alfalfa Forage Through Inorganic Selenium Foliar Application: Insights from Laboratory and Field Studies Using X-Ray Spectroscopy |
| title_full | Enhancing Organic Selenium Forms in Alfalfa Forage Through Inorganic Selenium Foliar Application: Insights from Laboratory and Field Studies Using X-Ray Spectroscopy |
| title_fullStr | Enhancing Organic Selenium Forms in Alfalfa Forage Through Inorganic Selenium Foliar Application: Insights from Laboratory and Field Studies Using X-Ray Spectroscopy |
| title_full_unstemmed | Enhancing Organic Selenium Forms in Alfalfa Forage Through Inorganic Selenium Foliar Application: Insights from Laboratory and Field Studies Using X-Ray Spectroscopy |
| title_short | Enhancing Organic Selenium Forms in Alfalfa Forage Through Inorganic Selenium Foliar Application: Insights from Laboratory and Field Studies Using X-Ray Spectroscopy |
| title_sort | enhancing organic selenium forms in alfalfa forage through inorganic selenium foliar application insights from laboratory and field studies using x ray spectroscopy |
| topic | Selenio Experimentación en Laboratorio Experimentación en Campo Espectros Copia de Rayos X Espectroscopia Selenium Medicago sativa Laboratory Experimentation Field Experimentation X-ray Spectroscopy Spectroscopy Alfalfa Lucerne |
| url | http://hdl.handle.net/20.500.12123/21517 https://www.mdpi.com/2073-4395/15/3/580 https://doi.org/10.3390/agronomy15030580 |
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