Colistin-resistant Escherichia coli mediated by the mcr-1 gene from pigs in northeastern Argentina = Resistencia a colistina mediada por mcr-1 en Escherichia coli aislada de cerdos del nordeste argentino
The emergence and spread of multidrug-resistant Escherichia coli carrying mcr-1 is recognized as a threat to public health. The aim of this study was to determine the prevalence of the mcr-1 gene in colistin-resistant E. coli isolates from commercial pig farms in Chaco, Argentina from 2020 to 2021....
| Autores principales: | , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2025
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/21510 https://www.sciencedirect.com/science/article/pii/S0325754125000070 https://doi.org/10.1016/j.ram.2024.12.013 |
| _version_ | 1855038412907610112 |
|---|---|
| author | Pellegrini, Juan Leandro Gonzalez, Maria De Los Angeles Lösch, Liliana Silvina Merino, Luis Antonio Di Conza, José Alejandro |
| author_browse | Di Conza, José Alejandro Gonzalez, Maria De Los Angeles Lösch, Liliana Silvina Merino, Luis Antonio Pellegrini, Juan Leandro |
| author_facet | Pellegrini, Juan Leandro Gonzalez, Maria De Los Angeles Lösch, Liliana Silvina Merino, Luis Antonio Di Conza, José Alejandro |
| author_sort | Pellegrini, Juan Leandro |
| collection | INTA Digital |
| description | The emergence and spread of multidrug-resistant Escherichia coli carrying mcr-1 is recognized as a threat to public health. The aim of this study was to determine the prevalence of the mcr-1 gene in colistin-resistant E. coli isolates from commercial pig farms in Chaco, Argentina from 2020 to 2021. A total of 140 rectal swab samples were collected from pigs in six different pig production farms. Antimicrobial susceptibility was determined by broth microdilution. mcr-1 to mcr-5 genes were identified by multiplex PCR and clonality was assessed by ERIC and REP-PCR. The prevalence of mcr-1 was 16.4% and mcr-2, mcr-3, mcr-4 and mcr-5 genes were not detected. Colistin MIC values showed a bimodal distribution with a MIC50, MIC90 and a range of 4, 8 and 4–8 μg/ml, respectively. The resistance profile to other antimicrobials was: ampicillin, 87% (20); ampicillin–sulbactam, 47.8% (11); amoxicillin–clavulanic, 13% (3); chloramphenicol, 82.6% (19); ciprofloxacin, 60.9% (14); minocycline, 26.1% (5) and trimethoprim/sulfamethoxazole, 43.5% (10). Eighty-seven percent (87%) of the strains were categorized as MDR and 12 phenotypic resistance patterns with different clonality profiles were observed. A high prevalence of mcr-1 is demonstrated in colistin-free pig farms from Chaco, Argentina. The mcr-1 positive E. coli isolates showed an alarming level of multidrug resistance and high clonal diversity. It is necessary to continuously monitor the presence of the mcr-1 gene not only in pig production, but also in humans and the environment. |
| format | info:ar-repo/semantics/artículo |
| id | INTA21510 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA215102025-02-28T12:40:35Z Colistin-resistant Escherichia coli mediated by the mcr-1 gene from pigs in northeastern Argentina = Resistencia a colistina mediada por mcr-1 en Escherichia coli aislada de cerdos del nordeste argentino Pellegrini, Juan Leandro Gonzalez, Maria De Los Angeles Lösch, Liliana Silvina Merino, Luis Antonio Di Conza, José Alejandro Escherichia coli Colistin Swine Antimicrobial Resistance Colistina Cerdo Resistencia a los Antimicrobianos Región Noreste, Argentina The emergence and spread of multidrug-resistant Escherichia coli carrying mcr-1 is recognized as a threat to public health. The aim of this study was to determine the prevalence of the mcr-1 gene in colistin-resistant E. coli isolates from commercial pig farms in Chaco, Argentina from 2020 to 2021. A total of 140 rectal swab samples were collected from pigs in six different pig production farms. Antimicrobial susceptibility was determined by broth microdilution. mcr-1 to mcr-5 genes were identified by multiplex PCR and clonality was assessed by ERIC and REP-PCR. The prevalence of mcr-1 was 16.4% and mcr-2, mcr-3, mcr-4 and mcr-5 genes were not detected. Colistin MIC values showed a bimodal distribution with a MIC50, MIC90 and a range of 4, 8 and 4–8 μg/ml, respectively. The resistance profile to other antimicrobials was: ampicillin, 87% (20); ampicillin–sulbactam, 47.8% (11); amoxicillin–clavulanic, 13% (3); chloramphenicol, 82.6% (19); ciprofloxacin, 60.9% (14); minocycline, 26.1% (5) and trimethoprim/sulfamethoxazole, 43.5% (10). Eighty-seven percent (87%) of the strains were categorized as MDR and 12 phenotypic resistance patterns with different clonality profiles were observed. A high prevalence of mcr-1 is demonstrated in colistin-free pig farms from Chaco, Argentina. The mcr-1 positive E. coli isolates showed an alarming level of multidrug resistance and high clonal diversity. It is necessary to continuously monitor the presence of the mcr-1 gene not only in pig production, but also in humans and the environment. La aparición y propagación de Escherichia coli resistente a múltiples fármacos, portadora de mcr-1, se reconoce como una amenaza para la salud pública. El objetivo de este estudio fue determinar la prevalencia del gen mcr-1 en aislamientos de E. coli resistentes a colistina en granjas porcinas de Chaco, Argentina. Se recolectaron 140 muestras de hisopados rectales de cerdos en seis granjas de producción entre marzo de 2020 y julio de 2021. La sensibilidad antimicrobiana se evaluó mediante el método de microdilución en caldo. Los genes mcr-1, mcr-2, mcr-3, mcr-4 y mcr-5 se identificaron por PCR multiplex; la clonalidad se evaluó mediante ERIC y REP-PCR. La prevalencia de mcr-1 fue del 16,4% y no se detectaron los genes mcr-2, mcr-3, mcr-4 y mcr-5. Los valores de CIM de colistina presentaron una distribución bimodal, con unas CIM50 y CIM90 de 4 y 8 μg/ml, respectivamente, y un rango de 4-8 μg/ml. El perfil de resistencia a otros antimicrobianos dentro de los aislamientos mcr-1 positivos fue el siguiente: ampicilina, 87% (20); ampicilina-sulbactam, 47,8% (11); amoxicilina-clavulánico, 13% (3); cloranfenicol, 82,6% (19); ciprofloxacina, 60,9% (14); minociclina, 26,1% (5) y trimetoprima/sulfametoxazol, 43,5% (10). El 87% de las cepas se categorizaron como multidrogorresistentes (MDR) y se observaron 12 patrones fenotípicos de resistencia con diferentes perfiles de clonalidad. Se corroboró una elevada prevalencia de mcr-1 en granjas porcinas de Chaco libres de colistina. Los aislamientos de E. coli positivos para mcr-1 mostraron un nivel alarmante de multirresistencia y una alta diversidad clonal. Es necesario monitorear continuamente la presencia del gen mcr-1, no solo en la producción porcina, sino también en humanos y en el ambiente. EEA Las Breñas Fil: Pellegrini, Juan Leandro. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina Fil: Gonzalez, Maria De Los Angeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Las Breñas; Argentina Fil: Lösch, Liliana Silvina. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina Fil: Merino, Luis Antonio. Universidad Nacional del Nordeste. Instituto de Medicina Regional; Argentina Fil: Di Conza, José Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Di Conza, José Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-02-28T12:37:41Z 2025-02-28T12:37:41Z 2025-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/21510 https://www.sciencedirect.com/science/article/pii/S0325754125000070 0325-7541 1851-7617 https://doi.org/10.1016/j.ram.2024.12.013 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Revista Argentina de Microbiología : 1-7 (Available online 20 February 2025) |
| spellingShingle | Escherichia coli Colistin Swine Antimicrobial Resistance Colistina Cerdo Resistencia a los Antimicrobianos Región Noreste, Argentina Pellegrini, Juan Leandro Gonzalez, Maria De Los Angeles Lösch, Liliana Silvina Merino, Luis Antonio Di Conza, José Alejandro Colistin-resistant Escherichia coli mediated by the mcr-1 gene from pigs in northeastern Argentina = Resistencia a colistina mediada por mcr-1 en Escherichia coli aislada de cerdos del nordeste argentino |
| title | Colistin-resistant Escherichia coli mediated by the mcr-1 gene from pigs in northeastern Argentina = Resistencia a colistina mediada por mcr-1 en Escherichia coli aislada de cerdos del nordeste argentino |
| title_full | Colistin-resistant Escherichia coli mediated by the mcr-1 gene from pigs in northeastern Argentina = Resistencia a colistina mediada por mcr-1 en Escherichia coli aislada de cerdos del nordeste argentino |
| title_fullStr | Colistin-resistant Escherichia coli mediated by the mcr-1 gene from pigs in northeastern Argentina = Resistencia a colistina mediada por mcr-1 en Escherichia coli aislada de cerdos del nordeste argentino |
| title_full_unstemmed | Colistin-resistant Escherichia coli mediated by the mcr-1 gene from pigs in northeastern Argentina = Resistencia a colistina mediada por mcr-1 en Escherichia coli aislada de cerdos del nordeste argentino |
| title_short | Colistin-resistant Escherichia coli mediated by the mcr-1 gene from pigs in northeastern Argentina = Resistencia a colistina mediada por mcr-1 en Escherichia coli aislada de cerdos del nordeste argentino |
| title_sort | colistin resistant escherichia coli mediated by the mcr 1 gene from pigs in northeastern argentina resistencia a colistina mediada por mcr 1 en escherichia coli aislada de cerdos del nordeste argentino |
| topic | Escherichia coli Colistin Swine Antimicrobial Resistance Colistina Cerdo Resistencia a los Antimicrobianos Región Noreste, Argentina |
| url | http://hdl.handle.net/20.500.12123/21510 https://www.sciencedirect.com/science/article/pii/S0325754125000070 https://doi.org/10.1016/j.ram.2024.12.013 |
| work_keys_str_mv | AT pellegrinijuanleandro colistinresistantescherichiacolimediatedbythemcr1genefrompigsinnortheasternargentinaresistenciaacolistinamediadapormcr1enescherichiacoliaisladadecerdosdelnordesteargentino AT gonzalezmariadelosangeles colistinresistantescherichiacolimediatedbythemcr1genefrompigsinnortheasternargentinaresistenciaacolistinamediadapormcr1enescherichiacoliaisladadecerdosdelnordesteargentino AT loschlilianasilvina colistinresistantescherichiacolimediatedbythemcr1genefrompigsinnortheasternargentinaresistenciaacolistinamediadapormcr1enescherichiacoliaisladadecerdosdelnordesteargentino AT merinoluisantonio colistinresistantescherichiacolimediatedbythemcr1genefrompigsinnortheasternargentinaresistenciaacolistinamediadapormcr1enescherichiacoliaisladadecerdosdelnordesteargentino AT diconzajosealejandro colistinresistantescherichiacolimediatedbythemcr1genefrompigsinnortheasternargentinaresistenciaacolistinamediadapormcr1enescherichiacoliaisladadecerdosdelnordesteargentino |