Performance of the PhoP (Rv0757/Mb0780) protein as diagnostic antigen for bovine tuberculosis
Bovine tuberculosis (bTB), a global zoonotic disease, causes negative effects on human and animal health. PhoP protein is a key regulator of pathogenic phenotypes in members of the Mycobacterium tuberculosis complex, which includes the causative agent of bTB. Despite extensive research on this prote...
| Main Authors: | , , , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/21501 https://www.sciencedirect.com/science/article/abs/pii/S0034528824003801 https://doi.org/10.1016/j.rvsc.2024.105513 |
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| author | Ferrara Muñiz, Ximena Marques Da Silva, Wanderson Garbaccio, Sergio Gabriel Garro, Carlos Javier Sammarruco, Romina Ayelén Encinas, Micaela Carignano, Hugo Adrian Bianco, María Veronica Zumarraga, Martin Jose Cataldi, Angel Adrian Eirin, Maria Emilia |
| author_browse | Bianco, María Veronica Carignano, Hugo Adrian Cataldi, Angel Adrian Eirin, Maria Emilia Encinas, Micaela Ferrara Muñiz, Ximena Garbaccio, Sergio Gabriel Garro, Carlos Javier Marques Da Silva, Wanderson Sammarruco, Romina Ayelén Zumarraga, Martin Jose |
| author_facet | Ferrara Muñiz, Ximena Marques Da Silva, Wanderson Garbaccio, Sergio Gabriel Garro, Carlos Javier Sammarruco, Romina Ayelén Encinas, Micaela Carignano, Hugo Adrian Bianco, María Veronica Zumarraga, Martin Jose Cataldi, Angel Adrian Eirin, Maria Emilia |
| author_sort | Ferrara Muñiz, Ximena |
| collection | INTA Digital |
| description | Bovine tuberculosis (bTB), a global zoonotic disease, causes negative effects on human and animal health. PhoP protein is a key regulator of pathogenic phenotypes in members of the Mycobacterium tuberculosis complex, which includes the causative agent of bTB. Despite extensive research on this protein focused in deciphering its regulatory role, little was explored about it as a diagnostic antigen. In humans, a novel role of anti-PhoP antibodies as a possible marker for the diagnosis of TB was demonstrated. However, this issue was not addressed in bovines. In this study, antigenic properties of the PhoP protein were evaluated in naturally Mycobacterium bovis (M. bovis) infected bovines. A high homology of PhoP (≥ 75 %) was observed in environmental mycobacterial species and other genera such as Salmonella and Pasteurella. Using the IFN-gamma release assay (IGRA), we detected cell-mediated immune response against PhoP in cattle from infected herds (25 %; IC 95 % 3.2–65.1), although it was significantly lower than that evoked by the reference antigens, ESAT-6/CFP-10/Rv3615c (75 %; IC95 % 34.9–96.8), and the purified protein derivative (87.5 %; IC 95 % 47.4–99.7) (p < 0.05)). Animals from a bTB free area showed no response against PhoP when analyzed by IGRA. Although, the humoral response detected 62.5 % (CI95% 24.5–91.5) of naturally infected animals, there was 100 % cross-reactivity among TB-free cattle. These results suggest that the PhoP protein is not a promising candidate for bTB diagnosis, due to it had relatively low levels of test sensitivity in the IGRA test, and very low specificity in a humoral antibody western blot assay. |
| format | info:ar-repo/semantics/artículo |
| id | INTA21501 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA215012025-02-28T10:48:19Z Performance of the PhoP (Rv0757/Mb0780) protein as diagnostic antigen for bovine tuberculosis Ferrara Muñiz, Ximena Marques Da Silva, Wanderson Garbaccio, Sergio Gabriel Garro, Carlos Javier Sammarruco, Romina Ayelén Encinas, Micaela Carignano, Hugo Adrian Bianco, María Veronica Zumarraga, Martin Jose Cataldi, Angel Adrian Eirin, Maria Emilia Bovine Tuberculosis Immune Response Diagnostic Techniques Antigens Tuberculosis Bovina Respuesta Inmunológica Técnica de Diagnóstico Antígenos Mycobacterium bovis Bovine tuberculosis (bTB), a global zoonotic disease, causes negative effects on human and animal health. PhoP protein is a key regulator of pathogenic phenotypes in members of the Mycobacterium tuberculosis complex, which includes the causative agent of bTB. Despite extensive research on this protein focused in deciphering its regulatory role, little was explored about it as a diagnostic antigen. In humans, a novel role of anti-PhoP antibodies as a possible marker for the diagnosis of TB was demonstrated. However, this issue was not addressed in bovines. In this study, antigenic properties of the PhoP protein were evaluated in naturally Mycobacterium bovis (M. bovis) infected bovines. A high homology of PhoP (≥ 75 %) was observed in environmental mycobacterial species and other genera such as Salmonella and Pasteurella. Using the IFN-gamma release assay (IGRA), we detected cell-mediated immune response against PhoP in cattle from infected herds (25 %; IC 95 % 3.2–65.1), although it was significantly lower than that evoked by the reference antigens, ESAT-6/CFP-10/Rv3615c (75 %; IC95 % 34.9–96.8), and the purified protein derivative (87.5 %; IC 95 % 47.4–99.7) (p < 0.05)). Animals from a bTB free area showed no response against PhoP when analyzed by IGRA. Although, the humoral response detected 62.5 % (CI95% 24.5–91.5) of naturally infected animals, there was 100 % cross-reactivity among TB-free cattle. These results suggest that the PhoP protein is not a promising candidate for bTB diagnosis, due to it had relatively low levels of test sensitivity in the IGRA test, and very low specificity in a humoral antibody western blot assay. Instituto de Biotecnología Fil: Ferrara Muñiz, Ximena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ferrara Muñiz, Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Marques Da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Marques Da Silva, Wanderson. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Garbaccio, Sergio Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Garro, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sammarruco, Romina Ayelén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Sammarruco, Romina Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Encinas, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Encinas, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Carignano, Hugo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Carignano, Hugo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Bianco, María Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Eirin, Maria Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Eirin, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-02-28T10:36:31Z 2025-02-28T10:36:31Z 2025-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21501 https://www.sciencedirect.com/science/article/abs/pii/S0034528824003801 1532-2661 https://doi.org/10.1016/j.rvsc.2024.105513 eng info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Salud info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Research in Veterinary Science 184 : 105513 (March 2025) |
| spellingShingle | Bovine Tuberculosis Immune Response Diagnostic Techniques Antigens Tuberculosis Bovina Respuesta Inmunológica Técnica de Diagnóstico Antígenos Mycobacterium bovis Ferrara Muñiz, Ximena Marques Da Silva, Wanderson Garbaccio, Sergio Gabriel Garro, Carlos Javier Sammarruco, Romina Ayelén Encinas, Micaela Carignano, Hugo Adrian Bianco, María Veronica Zumarraga, Martin Jose Cataldi, Angel Adrian Eirin, Maria Emilia Performance of the PhoP (Rv0757/Mb0780) protein as diagnostic antigen for bovine tuberculosis |
| title | Performance of the PhoP (Rv0757/Mb0780) protein as diagnostic antigen for bovine tuberculosis |
| title_full | Performance of the PhoP (Rv0757/Mb0780) protein as diagnostic antigen for bovine tuberculosis |
| title_fullStr | Performance of the PhoP (Rv0757/Mb0780) protein as diagnostic antigen for bovine tuberculosis |
| title_full_unstemmed | Performance of the PhoP (Rv0757/Mb0780) protein as diagnostic antigen for bovine tuberculosis |
| title_short | Performance of the PhoP (Rv0757/Mb0780) protein as diagnostic antigen for bovine tuberculosis |
| title_sort | performance of the phop rv0757 mb0780 protein as diagnostic antigen for bovine tuberculosis |
| topic | Bovine Tuberculosis Immune Response Diagnostic Techniques Antigens Tuberculosis Bovina Respuesta Inmunológica Técnica de Diagnóstico Antígenos Mycobacterium bovis |
| url | http://hdl.handle.net/20.500.12123/21501 https://www.sciencedirect.com/science/article/abs/pii/S0034528824003801 https://doi.org/10.1016/j.rvsc.2024.105513 |
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