Reduction of in vitro viral replication of caprine alphaherpesvirus 1 (CPHV-1) through natural antiviral treatment with plant extracts = Reducción de la replicación viral in vitro de alfaherpesvirus caprino 1 (CpHV-1) por tratamiento antiviral natural de extractos de plantas
Caprine herpesvirus 1 (CpHV-1) is distributed worldwide, causing significant economic losses. The application of acyclovir interferes with viral replication to varying degrees of efficacy, but it does not prevent the establishment of latency. Due to the costs and side effects associated with synthet...
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| Format: | Artículo |
| Language: | Inglés |
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Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/21497 https://revistas.unne.edu.ar/index.php/vet/article/view/8088 https://doi.org/10.30972/vet.3618088 |
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| author | Ferreccio, Carola Maria Maidana, Silvina Soledad Tau, Rocio Lucia Di Ciaccio, Lucia Soledad Spotorno, Viviana Graciela Salvat, Adriana Elisabeth Aguilar, Juan Javier Konigheim, Brenda Romera, Sonia Alejandra |
| author_browse | Aguilar, Juan Javier Di Ciaccio, Lucia Soledad Ferreccio, Carola Maria Konigheim, Brenda Maidana, Silvina Soledad Romera, Sonia Alejandra Salvat, Adriana Elisabeth Spotorno, Viviana Graciela Tau, Rocio Lucia |
| author_facet | Ferreccio, Carola Maria Maidana, Silvina Soledad Tau, Rocio Lucia Di Ciaccio, Lucia Soledad Spotorno, Viviana Graciela Salvat, Adriana Elisabeth Aguilar, Juan Javier Konigheim, Brenda Romera, Sonia Alejandra |
| author_sort | Ferreccio, Carola Maria |
| collection | INTA Digital |
| description | Caprine herpesvirus 1 (CpHV-1) is distributed worldwide, causing significant economic losses. The application of acyclovir interferes with viral replication to varying degrees of efficacy, but it does not prevent the establishment of latency. Due to the costs and side effects associated with synthetic drugs, the search for new chemotherapeutic agents is essential for the treatment and control of viral diseases. The objective of this study is to evaluate and compare the in vitro antiviral activity of three native plants from Argentina as a therapeutic antiviral treatment against CpHV-1. Larrea divaricata, Minthostachys verticillata and Parastrephia quadrangularis were used. Four parameters were analyzed: Cytotoxic Concentration 50 (CC50), Inhibitory Concentration 50 (IC50), quantification by plaque assay and viral titration. The L. divaricata extract showed the lowest cytotoxicity (2.4 mg ml-1). The best selectivity index (SI) was obtained with the M. verticillata extract (10). L. divaricata and P. quadrangularis extracts demonstrated the greatest antiviral effects, with reductions of 90% and 75%, respectively, compared to untreated controls, where the greatest reduction in plaque size was observed. The highest inhibition of viral replication observed in the viral titration quantification occurred when monolayers were treated with L. divaricata, resulting in at least a 35% reduction. The combination of assay with L. divaricata and M. verticillata showed the greatest reduction in viral titers. The overall results provide evidence that L. divaricata and M. verticillata could serve as potential sources for new anti-CpHV-1 drugs. |
| format | Artículo |
| id | INTA21497 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste |
| publisherStr | Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste |
| record_format | dspace |
| spelling | INTA214972025-02-28T10:02:23Z Reduction of in vitro viral replication of caprine alphaherpesvirus 1 (CPHV-1) through natural antiviral treatment with plant extracts = Reducción de la replicación viral in vitro de alfaherpesvirus caprino 1 (CpHV-1) por tratamiento antiviral natural de extractos de plantas Ferreccio, Carola Maria Maidana, Silvina Soledad Tau, Rocio Lucia Di Ciaccio, Lucia Soledad Spotorno, Viviana Graciela Salvat, Adriana Elisabeth Aguilar, Juan Javier Konigheim, Brenda Romera, Sonia Alejandra Herpesviridae In vitro Plant Extracts Antiviral Plants Argentina Planta Antiviral Extractos Vegetales Caprine Herpesvirus Herpesvirus Caprino Larrea divaricata Minthostachys verticillata Parastrephia quadrangularis Caprine herpesvirus 1 (CpHV-1) is distributed worldwide, causing significant economic losses. The application of acyclovir interferes with viral replication to varying degrees of efficacy, but it does not prevent the establishment of latency. Due to the costs and side effects associated with synthetic drugs, the search for new chemotherapeutic agents is essential for the treatment and control of viral diseases. The objective of this study is to evaluate and compare the in vitro antiviral activity of three native plants from Argentina as a therapeutic antiviral treatment against CpHV-1. Larrea divaricata, Minthostachys verticillata and Parastrephia quadrangularis were used. Four parameters were analyzed: Cytotoxic Concentration 50 (CC50), Inhibitory Concentration 50 (IC50), quantification by plaque assay and viral titration. The L. divaricata extract showed the lowest cytotoxicity (2.4 mg ml-1). The best selectivity index (SI) was obtained with the M. verticillata extract (10). L. divaricata and P. quadrangularis extracts demonstrated the greatest antiviral effects, with reductions of 90% and 75%, respectively, compared to untreated controls, where the greatest reduction in plaque size was observed. The highest inhibition of viral replication observed in the viral titration quantification occurred when monolayers were treated with L. divaricata, resulting in at least a 35% reduction. The combination of assay with L. divaricata and M. verticillata showed the greatest reduction in viral titers. The overall results provide evidence that L. divaricata and M. verticillata could serve as potential sources for new anti-CpHV-1 drugs. El herpesvirus caprino 1 (CpHV-1) se distribuye mundialmente siendo responsable de pérdidas económicas. La aplicación de aciclovir interfiere en la replicación viral con distintos grados de eficacia, pero no impide el establecimiento de latencia. Debido a los costos y efectos secundarios que producen los fármacos antihipertensivos, la búsqueda de nuevos agentes quimioterapéuticos es fundamental para el tratamiento y control de las enfermedades virales. El objetivo del trabajo es evaluar y comparar la actividad antiviral in vitro de tres plantas nativas de Argentina como tratamiento antiviral terapéutico contra CpHV-1. Se utilizaron Larrea divaricata, Minthostachys verticillata y Parastrephia quadrangularis. Se analizaron cuatro parámetros: Concentración Citotóxica 50 (CC50), Concentración Inhibitoria 50 (CI50), cuantificación por ensayo de placa y titulación viral. El extracto de L. divaricata mostró la menor citotoxicidad (2,4 mg ml-1). El mejor SI (índice de selectividad) se obtuvo en el extracto de M. verticillata (10). Los extractos de L. divaricata y P. quadrangularis son los compuestos que mostraron el mayor efecto antiviral (90% y 75%, respectivamente), en comparación con los controles no tratados, en los que se observó la mayor reducción en el tamaño de la placa. La mayor inhibición de la replicación viral se observó cuando se evaluó el título viral cuando las monocapas fueron tratadas con L. divaricata (al menos disminución del 35%). Con el ensayo de combinación de L. divaricata y M. verticillata se observó la mayor reducción de los títulos virales. Los resultados generales proporcionan evidencia de que L. divaricata y M. verticillata podrían ser fuentes potenciales de nuevos fármacos anti-CpHV-1. Instituto de Virología Fil: Ferrecio, Carola Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Maidana, Silvina Soledad. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias. Cátedra de Inmunología Aplicada; Argentina Fil: Tau, Rocio Lucia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Di Ciaccio, Lucía Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Spotorno, Viviana Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Spotorno, Viviana Graciela. Instituto de Virología Dr. JM Vanella; Argentina Fil: Salvat, Adriana Elisabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Aguilar, Juan Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Aguilar, Juan Javier. Instituto de Virología Dr. JM Vanella; Argentina Fil: Konigheim, Brenda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Konigheim, Brenda. Instituto de Virología Dr. JM Vanella; Argentina Fil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Romera, Sonia Alejandra. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias. Cátedra de Inmunología Aplicada; Argentina 2025-02-28T09:49:52Z 2025-02-28T09:49:52Z 2025-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21497 https://revistas.unne.edu.ar/index.php/vet/article/view/8088 1669-6840 https://doi.org/10.30972/vet.3618088 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste Revista Veterinaria / Universidad Nacional del Nordeste 36 (1) : 1-8 (Febrero 2025) |
| spellingShingle | Herpesviridae In vitro Plant Extracts Antiviral Plants Argentina Planta Antiviral Extractos Vegetales Caprine Herpesvirus Herpesvirus Caprino Larrea divaricata Minthostachys verticillata Parastrephia quadrangularis Ferreccio, Carola Maria Maidana, Silvina Soledad Tau, Rocio Lucia Di Ciaccio, Lucia Soledad Spotorno, Viviana Graciela Salvat, Adriana Elisabeth Aguilar, Juan Javier Konigheim, Brenda Romera, Sonia Alejandra Reduction of in vitro viral replication of caprine alphaherpesvirus 1 (CPHV-1) through natural antiviral treatment with plant extracts = Reducción de la replicación viral in vitro de alfaherpesvirus caprino 1 (CpHV-1) por tratamiento antiviral natural de extractos de plantas |
| title | Reduction of in vitro viral replication of caprine alphaherpesvirus 1 (CPHV-1) through natural antiviral treatment with plant extracts = Reducción de la replicación viral in vitro de alfaherpesvirus caprino 1 (CpHV-1) por tratamiento antiviral natural de extractos de plantas |
| title_full | Reduction of in vitro viral replication of caprine alphaherpesvirus 1 (CPHV-1) through natural antiviral treatment with plant extracts = Reducción de la replicación viral in vitro de alfaherpesvirus caprino 1 (CpHV-1) por tratamiento antiviral natural de extractos de plantas |
| title_fullStr | Reduction of in vitro viral replication of caprine alphaherpesvirus 1 (CPHV-1) through natural antiviral treatment with plant extracts = Reducción de la replicación viral in vitro de alfaherpesvirus caprino 1 (CpHV-1) por tratamiento antiviral natural de extractos de plantas |
| title_full_unstemmed | Reduction of in vitro viral replication of caprine alphaherpesvirus 1 (CPHV-1) through natural antiviral treatment with plant extracts = Reducción de la replicación viral in vitro de alfaherpesvirus caprino 1 (CpHV-1) por tratamiento antiviral natural de extractos de plantas |
| title_short | Reduction of in vitro viral replication of caprine alphaherpesvirus 1 (CPHV-1) through natural antiviral treatment with plant extracts = Reducción de la replicación viral in vitro de alfaherpesvirus caprino 1 (CpHV-1) por tratamiento antiviral natural de extractos de plantas |
| title_sort | reduction of in vitro viral replication of caprine alphaherpesvirus 1 cphv 1 through natural antiviral treatment with plant extracts reduccion de la replicacion viral in vitro de alfaherpesvirus caprino 1 cphv 1 por tratamiento antiviral natural de extractos de plantas |
| topic | Herpesviridae In vitro Plant Extracts Antiviral Plants Argentina Planta Antiviral Extractos Vegetales Caprine Herpesvirus Herpesvirus Caprino Larrea divaricata Minthostachys verticillata Parastrephia quadrangularis |
| url | http://hdl.handle.net/20.500.12123/21497 https://revistas.unne.edu.ar/index.php/vet/article/view/8088 https://doi.org/10.30972/vet.3618088 |
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