Effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop (Humulus lupulus L.)
Hops (Humulus lupulus L.) is a dioecious plant of the Cannabaceae family, highly valued in beer production. In vitro culture has been established as a tool in agricultural biotechnology for its propagation and improvement. This study evaluated the impact of different concentrations of cytokinins, to...
| Main Authors: | , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Taylor and Francis
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/21483 https://www.tandfonline.com/doi/10.1080/01140671.2025.2458812 https://doi.org/10.1080/01140671.2025.2458812 |
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| author | Carloni, Edgardo José Rodriguez, Milena Nahir Tommasino, Exequiel Arturo Ruolo, Maria Soledad Grunberg, Karina |
| author_browse | Carloni, Edgardo José Grunberg, Karina Rodriguez, Milena Nahir Ruolo, Maria Soledad Tommasino, Exequiel Arturo |
| author_facet | Carloni, Edgardo José Rodriguez, Milena Nahir Tommasino, Exequiel Arturo Ruolo, Maria Soledad Grunberg, Karina |
| author_sort | Carloni, Edgardo José |
| collection | INTA Digital |
| description | Hops (Humulus lupulus L.) is a dioecious plant of the Cannabaceae family, highly valued in beer production. In vitro culture has been established as a tool in agricultural biotechnology for its propagation and improvement. This study evaluated the impact of different concentrations of cytokinins, to develop an efficient protocol for in vitro micropropagation of the species. Concentrations of 0.1, 1, 3, and 6 mg·L−1 of BAP and KIN and control without cytokinins were used. After 28 days of culture, growth and development variables were analysed. The results indicated that lower concentrations of cytokinins favoured most of the variables studied. In contrast, concentrations equal to or higher than 1 mg·L−1 of BAP and KIN reduced growth, and increased callus formation and hyperhydricity. The protocol developed proved efficient, with more than 90% of the explants generating at least two shoots and approximately the same percentage developing roots. In addition, seedlings obtained with the 0.1 mg·L−1 KIN and BAP treatment showed high survival after transplanting. Applying low concentrations of cytokinins eliminated the need for an additional rooting stage, optimising the micropropagation process |
| format | Artículo |
| id | INTA21483 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Taylor and Francis |
| publisherStr | Taylor and Francis |
| record_format | dspace |
| spelling | INTA214832025-02-27T10:45:29Z Effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop (Humulus lupulus L.) Carloni, Edgardo José Rodriguez, Milena Nahir Tommasino, Exequiel Arturo Ruolo, Maria Soledad Grunberg, Karina 6-benzylaminopurine Kinetin Callus Hops 6-benziladenina Quinetina Callo Lúpulo Humulus lupulus Micropropagation Protocol Lupulin Hyperhydricity Hops (Humulus lupulus L.) is a dioecious plant of the Cannabaceae family, highly valued in beer production. In vitro culture has been established as a tool in agricultural biotechnology for its propagation and improvement. This study evaluated the impact of different concentrations of cytokinins, to develop an efficient protocol for in vitro micropropagation of the species. Concentrations of 0.1, 1, 3, and 6 mg·L−1 of BAP and KIN and control without cytokinins were used. After 28 days of culture, growth and development variables were analysed. The results indicated that lower concentrations of cytokinins favoured most of the variables studied. In contrast, concentrations equal to or higher than 1 mg·L−1 of BAP and KIN reduced growth, and increased callus formation and hyperhydricity. The protocol developed proved efficient, with more than 90% of the explants generating at least two shoots and approximately the same percentage developing roots. In addition, seedlings obtained with the 0.1 mg·L−1 KIN and BAP treatment showed high survival after transplanting. Applying low concentrations of cytokinins eliminated the need for an additional rooting stage, optimising the micropropagation process Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Carloni, Edgardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina Fil: Rodriguez, Milena Nahir. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Rodriguez, Milena Nahir.Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina Fil: Tommasino, Exequiel Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Ruolo, Maria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Ruolo, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina Fil: Ruolo, Maria Soledad. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina Fil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Grunberg, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina 2025-02-27T10:29:54Z 2025-02-27T10:29:54Z 2025-01-21 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21483 https://www.tandfonline.com/doi/10.1080/01140671.2025.2458812 0114-0671 1175-8783 https://doi.org/10.1080/01140671.2025.2458812 eng info:eu-repograntAgreement/INTA/2019-RIST-E4-I091-001, Multiplicación de propágulos de sanidad controlada info:eu-repograntAgreement/INTA/2023-PD-L01-I071, Mejoramiento genético de los Cultivos Industriales y estrategias alternativas para la obtención de biotipos adaptados a los nuevos escenarios y al cambio climático info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Taylor and Francis New Zealand Journal of Crop and Horticultural Science (Published online: 05 Feb 2025) |
| spellingShingle | 6-benzylaminopurine Kinetin Callus Hops 6-benziladenina Quinetina Callo Lúpulo Humulus lupulus Micropropagation Protocol Lupulin Hyperhydricity Carloni, Edgardo José Rodriguez, Milena Nahir Tommasino, Exequiel Arturo Ruolo, Maria Soledad Grunberg, Karina Effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop (Humulus lupulus L.) |
| title | Effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop (Humulus lupulus L.) |
| title_full | Effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop (Humulus lupulus L.) |
| title_fullStr | Effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop (Humulus lupulus L.) |
| title_full_unstemmed | Effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop (Humulus lupulus L.) |
| title_short | Effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop (Humulus lupulus L.) |
| title_sort | effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop humulus lupulus l |
| topic | 6-benzylaminopurine Kinetin Callus Hops 6-benziladenina Quinetina Callo Lúpulo Humulus lupulus Micropropagation Protocol Lupulin Hyperhydricity |
| url | http://hdl.handle.net/20.500.12123/21483 https://www.tandfonline.com/doi/10.1080/01140671.2025.2458812 https://doi.org/10.1080/01140671.2025.2458812 |
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