Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae Kleb
Plants infected by fungal pathogens often actively recruit microbes in their roots to protect themselves. It is widely accepted that the plant microbiome plays a crucial role to sustain the fitness, resilience and development of the plant holobiont, and that the host actively shapes the rhizosphere...
| Main Authors: | , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Elsevier
2025
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/21478 https://www.sciencedirect.com/science/article/abs/pii/S0929139325000538 https://doi.org/10.1016/j.apsoil.2025.105915 |
| _version_ | 1855486760008548352 |
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| author | Ben Guerrero, Emiliano Lasa, Ana V. Aguilera, Pablo Nicolás Fernández-González, Antonio J. Martinez, Maria Carolina Mercado Blanco, Jesús Fernández-López, Manuel Paniego, Norma Beatriz |
| author_browse | Aguilera, Pablo Nicolás Ben Guerrero, Emiliano Fernández-González, Antonio J. Fernández-López, Manuel Lasa, Ana V. Martinez, Maria Carolina Mercado Blanco, Jesús Paniego, Norma Beatriz |
| author_facet | Ben Guerrero, Emiliano Lasa, Ana V. Aguilera, Pablo Nicolás Fernández-González, Antonio J. Martinez, Maria Carolina Mercado Blanco, Jesús Fernández-López, Manuel Paniego, Norma Beatriz |
| author_sort | Ben Guerrero, Emiliano |
| collection | INTA Digital |
| description | Plants infected by fungal pathogens often actively recruit microbes in their roots to protect themselves. It is widely accepted that the plant microbiome plays a crucial role to sustain the fitness, resilience and development of the plant holobiont, and that the host actively shapes the rhizosphere microbiome to prevent or suppress soil borne diseases. Here, we studied the rhizosphere bacterial communities of three sunflower inbred lines (ILs) with different level of resistance to Verticillium dahliae Kleb. We used 16S rRNA gene amplicon sequencing to profile bacterial communities before and after inoculation with V. dahliae. Overall, there was no significant association of alpha diversity indices with sunflower ILs and inoculation of the pathogen. However, a moderate increase in the richness and diversity was observed with the increment of resistance. In contrast, there were clear differences in the rhizosphere bacterial community structures related to the level of susceptibility of the sunflower genotypes. The predominant phyla comprised Proteobacteria, Bacteroidetes, and Acidobacteria. At the genus level, Rhodanobacter, Chujaibacter, Flavitalea, Lysobacter, Devosia, Bryobacter, Dokdonella and Bradyrhizobium were the most abundant genera in all cases. Our results indicate that sunflower genotype and V. dahliae infection of roots led to considerable changes in the composition of the rhizosphere bacterial communities. Changes were mostly observed in the relative abundances of core microbiome members, which varied significantly and differentially depending on the sunflower ILs and its level of resistance to V. dahliae. Thus, harnessing sunflower-associated rhizosphere bacteriomes for disease control may offer a valuable alternative strategy to increase the productivity and sustainability of agricultural production for this crop. |
| format | Artículo |
| id | INTA21478 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA214782025-02-26T15:04:57Z Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae Kleb Ben Guerrero, Emiliano Lasa, Ana V. Aguilera, Pablo Nicolás Fernández-González, Antonio J. Martinez, Maria Carolina Mercado Blanco, Jesús Fernández-López, Manuel Paniego, Norma Beatriz Verticillium Wilt Disease Resistance Genotypes Metabarcoding Ribosomal RNA Microbiomes Rhizosphere Sunflowers Marchitez por Verticillium Resistencia a la Enfermedad Genotipos Metabarcodificación Arn Ribosomial Microbioma Rizosfera Girasol Helianthus annuus Lysobacter Plants infected by fungal pathogens often actively recruit microbes in their roots to protect themselves. It is widely accepted that the plant microbiome plays a crucial role to sustain the fitness, resilience and development of the plant holobiont, and that the host actively shapes the rhizosphere microbiome to prevent or suppress soil borne diseases. Here, we studied the rhizosphere bacterial communities of three sunflower inbred lines (ILs) with different level of resistance to Verticillium dahliae Kleb. We used 16S rRNA gene amplicon sequencing to profile bacterial communities before and after inoculation with V. dahliae. Overall, there was no significant association of alpha diversity indices with sunflower ILs and inoculation of the pathogen. However, a moderate increase in the richness and diversity was observed with the increment of resistance. In contrast, there were clear differences in the rhizosphere bacterial community structures related to the level of susceptibility of the sunflower genotypes. The predominant phyla comprised Proteobacteria, Bacteroidetes, and Acidobacteria. At the genus level, Rhodanobacter, Chujaibacter, Flavitalea, Lysobacter, Devosia, Bryobacter, Dokdonella and Bradyrhizobium were the most abundant genera in all cases. Our results indicate that sunflower genotype and V. dahliae infection of roots led to considerable changes in the composition of the rhizosphere bacterial communities. Changes were mostly observed in the relative abundances of core microbiome members, which varied significantly and differentially depending on the sunflower ILs and its level of resistance to V. dahliae. Thus, harnessing sunflower-associated rhizosphere bacteriomes for disease control may offer a valuable alternative strategy to increase the productivity and sustainability of agricultural production for this crop. Instituto de Biotecnología Fil: Ben Guerrero, Emiliano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ben Guerrero, Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ben Guerrero, Emiliano. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; España Fil: Lasa, Ana V. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; España Fil: Aguilera, Pablo Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Aguilera, Pablo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fernández-González, Antonio J. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; España Fil: Martinez, Maria Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Martinez, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mercado Blanco, Jesús. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; España Fil: Fernández-López, Manuel. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Department of Soil and Plant Microbiology; España Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Paniego, Norma Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-02-26T14:59:50Z 2025-02-26T14:59:50Z 2025-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21478 https://www.sciencedirect.com/science/article/abs/pii/S0929139325000538 0929-1393 https://doi.org/10.1016/j.apsoil.2025.105915 eng info:eu-repograntAgreement/INTA/2023-PD-L01-I089, Microbiomas en ecosistemas agropecuarios: la conexión integradora del enfoque Una Salud info:eu-repograntAgreement/INTA/2023-PD-L01-I087, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada. info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Applied Soil Ecology 206 : 105915 (February 2025) |
| spellingShingle | Verticillium Wilt Disease Resistance Genotypes Metabarcoding Ribosomal RNA Microbiomes Rhizosphere Sunflowers Marchitez por Verticillium Resistencia a la Enfermedad Genotipos Metabarcodificación Arn Ribosomial Microbioma Rizosfera Girasol Helianthus annuus Lysobacter Ben Guerrero, Emiliano Lasa, Ana V. Aguilera, Pablo Nicolás Fernández-González, Antonio J. Martinez, Maria Carolina Mercado Blanco, Jesús Fernández-López, Manuel Paniego, Norma Beatriz Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae Kleb |
| title | Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae Kleb |
| title_full | Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae Kleb |
| title_fullStr | Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae Kleb |
| title_full_unstemmed | Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae Kleb |
| title_short | Unveiling changes in the rhizosphere bacteriome of sunflower (Helianthus annuus L.) inbred lines linked to their resistance to the soil borne pathogen Verticillium dahliae Kleb |
| title_sort | unveiling changes in the rhizosphere bacteriome of sunflower helianthus annuus l inbred lines linked to their resistance to the soil borne pathogen verticillium dahliae kleb |
| topic | Verticillium Wilt Disease Resistance Genotypes Metabarcoding Ribosomal RNA Microbiomes Rhizosphere Sunflowers Marchitez por Verticillium Resistencia a la Enfermedad Genotipos Metabarcodificación Arn Ribosomial Microbioma Rizosfera Girasol Helianthus annuus Lysobacter |
| url | http://hdl.handle.net/20.500.12123/21478 https://www.sciencedirect.com/science/article/abs/pii/S0929139325000538 https://doi.org/10.1016/j.apsoil.2025.105915 |
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