Production of functional bovine IL-22 in a mammalian episomal expression system
Interleukin 22 is a member of the interleukin-10 superfamily of cytokines. This protein has a dual role as an inflammatory and anti-inflammatory molecule dependent on the context. IL-22 is produced mainly by immune cells and seems to have non-hematopoietic cells as its target. In this work, we repor...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2025
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/21474 https://www.sciencedirect.com/science/article/abs/pii/S0165242724001491 https://doi.org/10.1016/j.vetimm.2024.110863 |
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| author | Klepp, Laura Ines Bigi, María Mercedes Villafañe, Luciana María Blanco, Federico Carlos Malinge, Pauline Bigi, Fabiana |
| author_browse | Bigi, Fabiana Bigi, María Mercedes Blanco, Federico Carlos Klepp, Laura Ines Malinge, Pauline Villafañe, Luciana María |
| author_facet | Klepp, Laura Ines Bigi, María Mercedes Villafañe, Luciana María Blanco, Federico Carlos Malinge, Pauline Bigi, Fabiana |
| author_sort | Klepp, Laura Ines |
| collection | INTA Digital |
| description | Interleukin 22 is a member of the interleukin-10 superfamily of cytokines. This protein has a dual role as an inflammatory and anti-inflammatory molecule dependent on the context. IL-22 is produced mainly by immune cells and seems to have non-hematopoietic cells as its target. In this work, we report the production of bovine IL-22 for the first time in a semi-stable expression system in mammalian cells. We showed that this recombinant IL-22 possesses biological activity in bovine macrophages infected with Mycobacterium bovis and is easy to produce in large quantities.
Given its role in the defence against infections, the IL-22 produced in this work has potential applications in scientific research as well as in immunotherapy to treat diseases in cattle. |
| format | Artículo |
| id | INTA21474 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA214742025-02-26T13:46:01Z Production of functional bovine IL-22 in a mammalian episomal expression system Klepp, Laura Ines Bigi, María Mercedes Villafañe, Luciana María Blanco, Federico Carlos Malinge, Pauline Bigi, Fabiana Cattle Mycobacterium bovis Cytokines Immunotherapy Ganado Bovino Citoquina Inmunoterapia Mammalian Cells Células de Mamíferos Interleukin 22 is a member of the interleukin-10 superfamily of cytokines. This protein has a dual role as an inflammatory and anti-inflammatory molecule dependent on the context. IL-22 is produced mainly by immune cells and seems to have non-hematopoietic cells as its target. In this work, we report the production of bovine IL-22 for the first time in a semi-stable expression system in mammalian cells. We showed that this recombinant IL-22 possesses biological activity in bovine macrophages infected with Mycobacterium bovis and is easy to produce in large quantities. Given its role in the defence against infections, the IL-22 produced in this work has potential applications in scientific research as well as in immunotherapy to treat diseases in cattle. Instituto de Biotecnología Fil: Klepp, Laura Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Klepp, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: Villafañe, Luciana María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Villafañe, Luciana María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Malinge, Pauline. Novimmune SA. Light Chain Bioscience; Suiza Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Bigi, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-02-26T13:39:07Z 2025-02-26T13:39:07Z 2025-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21474 https://www.sciencedirect.com/science/article/abs/pii/S0165242724001491 0165-2427 https://doi.org/10.1016/j.vetimm.2024.110863 eng info:eu-repograntAgreement/INTA/2023-PD-L06-I116, Implementación de tecnologías y nuevas estrategias preventivas y terapéuticas para el desarrollo sustentable y eficiente de la producción animal en el marco de Una Salud info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Veterinary Immunology and Immunopathology 279 : 110863 (January 2025) |
| spellingShingle | Cattle Mycobacterium bovis Cytokines Immunotherapy Ganado Bovino Citoquina Inmunoterapia Mammalian Cells Células de Mamíferos Klepp, Laura Ines Bigi, María Mercedes Villafañe, Luciana María Blanco, Federico Carlos Malinge, Pauline Bigi, Fabiana Production of functional bovine IL-22 in a mammalian episomal expression system |
| title | Production of functional bovine IL-22 in a mammalian episomal expression system |
| title_full | Production of functional bovine IL-22 in a mammalian episomal expression system |
| title_fullStr | Production of functional bovine IL-22 in a mammalian episomal expression system |
| title_full_unstemmed | Production of functional bovine IL-22 in a mammalian episomal expression system |
| title_short | Production of functional bovine IL-22 in a mammalian episomal expression system |
| title_sort | production of functional bovine il 22 in a mammalian episomal expression system |
| topic | Cattle Mycobacterium bovis Cytokines Immunotherapy Ganado Bovino Citoquina Inmunoterapia Mammalian Cells Células de Mamíferos |
| url | http://hdl.handle.net/20.500.12123/21474 https://www.sciencedirect.com/science/article/abs/pii/S0165242724001491 https://doi.org/10.1016/j.vetimm.2024.110863 |
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