A Novel Vaccine for Bovine Diarrhea Complex Utilizing Recombinant Enterotoxigenic Escherichia coli and Salmonella Expressing Surface-Displayed Chimeric Antigens from Enterohemorrhagic Escherichia coli O157:H7
Background/Objectives: Enterohemorrhagic Escherichia coli (EHEC) O157:H7, a zoonotic pathogen primarily found in cattle, causes Hemolytic Uremic Syndrome (HUS) in humans, often through contaminated food. Its Type Three Secretion System (T3SS) facilitates gut colonization. In contrast, neonatal calf...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/21473 https://www.mdpi.com/2076-393X/13/2/124 https://doi.org/10.3390/vaccines13020124 |
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| author | Ramírez, Hernán Vilte, Daniel Alejandro Hozbor, Daniela Flavia Zurita, Eugenia Bottero, Daniela Casabonne, Maria Carolina Cataldi, Angel Adrian Wigdorovitz, Andres Larzabal, Mariano |
| author_browse | Bottero, Daniela Casabonne, Maria Carolina Cataldi, Angel Adrian Hozbor, Daniela Flavia Larzabal, Mariano Ramírez, Hernán Vilte, Daniel Alejandro Wigdorovitz, Andres Zurita, Eugenia |
| author_facet | Ramírez, Hernán Vilte, Daniel Alejandro Hozbor, Daniela Flavia Zurita, Eugenia Bottero, Daniela Casabonne, Maria Carolina Cataldi, Angel Adrian Wigdorovitz, Andres Larzabal, Mariano |
| author_sort | Ramírez, Hernán |
| collection | INTA Digital |
| description | Background/Objectives: Enterohemorrhagic Escherichia coli (EHEC) O157:H7, a zoonotic pathogen primarily found in cattle, causes Hemolytic Uremic Syndrome (HUS) in humans, often through contaminated food. Its Type Three Secretion System (T3SS) facilitates gut colonization. In contrast, neonatal calf diarrhea (NCD) is mainly caused by pathogens like enterotoxigenic Escherichia coli (ETEC), Salmonella spp., Bovine Coronavirus (BCoV), and Bovine Rotavirus type A (BRoVA). This study engineered a chimeric protein combining EspB and Int280γ, two T3SS components, expressed in the membranes of Salmonella Dublin and ETEC. Methods: Immune responses in vaccinated mice and guinea pigs were assessed through ELISA assays. Results: Successful membrane anchorage and stability of the chimera were confirmed. Immune evaluations showed no enhancement from combining recombinant bacteria, indicating either bacterium suffices in a single formulation. Chimeric expression yielded immunogenicity equivalent to 10 µg of recombinant protein, with similar antibody titers. IgG1/IgG2a levels and Th1, Th2, and Th17 markers indicated a mixed immune response, providing broad humoral and cellular protection. Responses to BCoV, BRoVA, ETEC, and Salmonella antigens remained strong and did not interfere with chimera-specific responses, potentially boosting NCD vaccine efficacy. Conclusions: The chimera demonstrated robust immunogenicity, supporting its potential as a viable vaccine candidate against EHEC O157:H7. This approach could enhance NCD vaccine valency by offering broader protection against calf diarrhea while reducing HUS transmission risks to humans. |
| format | Artículo |
| id | INTA21473 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA214732025-02-26T13:39:49Z A Novel Vaccine for Bovine Diarrhea Complex Utilizing Recombinant Enterotoxigenic Escherichia coli and Salmonella Expressing Surface-Displayed Chimeric Antigens from Enterohemorrhagic Escherichia coli O157:H7 Ramírez, Hernán Vilte, Daniel Alejandro Hozbor, Daniela Flavia Zurita, Eugenia Bottero, Daniela Casabonne, Maria Carolina Cataldi, Angel Adrian Wigdorovitz, Andres Larzabal, Mariano Enfermedades de los Animales Animal Diseases Vacuna Vaccines Diarrea Viral Bovina Bovine Viral Diarrhoea Respuesta Inmunológica Immune Response Escherichia coli Salmonella Vacuna Sintética Synthetic Vaccines Background/Objectives: Enterohemorrhagic Escherichia coli (EHEC) O157:H7, a zoonotic pathogen primarily found in cattle, causes Hemolytic Uremic Syndrome (HUS) in humans, often through contaminated food. Its Type Three Secretion System (T3SS) facilitates gut colonization. In contrast, neonatal calf diarrhea (NCD) is mainly caused by pathogens like enterotoxigenic Escherichia coli (ETEC), Salmonella spp., Bovine Coronavirus (BCoV), and Bovine Rotavirus type A (BRoVA). This study engineered a chimeric protein combining EspB and Int280γ, two T3SS components, expressed in the membranes of Salmonella Dublin and ETEC. Methods: Immune responses in vaccinated mice and guinea pigs were assessed through ELISA assays. Results: Successful membrane anchorage and stability of the chimera were confirmed. Immune evaluations showed no enhancement from combining recombinant bacteria, indicating either bacterium suffices in a single formulation. Chimeric expression yielded immunogenicity equivalent to 10 µg of recombinant protein, with similar antibody titers. IgG1/IgG2a levels and Th1, Th2, and Th17 markers indicated a mixed immune response, providing broad humoral and cellular protection. Responses to BCoV, BRoVA, ETEC, and Salmonella antigens remained strong and did not interfere with chimera-specific responses, potentially boosting NCD vaccine efficacy. Conclusions: The chimera demonstrated robust immunogenicity, supporting its potential as a viable vaccine candidate against EHEC O157:H7. This approach could enhance NCD vaccine valency by offering broader protection against calf diarrhea while reducing HUS transmission risks to humans. Instituto de Patobiología Fil: Ramírez, Hernán. Bioinnovo S.A.; Argentina Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Vilte, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Patobiología Veterinaria; Argentina Fil: Hozbor, Daniela Flavia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Laboratorio VacSal; Argentina Fil: Hozbor, Daniela Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zurita, Eugenia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Laboratorio VacSal; Argentina Fil: Zurita, Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bottero, Daniela. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Laboratorio VacSal; Argentina Fil: Bottero, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Casabonne, María C. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Casabonne, María C. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Wigdorovitz, Andres. Bioinnovo S.A.; Argentina Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas (IVIT),; Argentina Fil: Wigdorovitz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina 2025-02-26T13:34:46Z 2025-02-26T13:34:46Z 2025-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21473 https://www.mdpi.com/2076-393X/13/2/124 2076-393X https://doi.org/10.3390/vaccines13020124 eng info:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Salud info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Vaccines 13 (2) : 124. (February 2025) |
| spellingShingle | Enfermedades de los Animales Animal Diseases Vacuna Vaccines Diarrea Viral Bovina Bovine Viral Diarrhoea Respuesta Inmunológica Immune Response Escherichia coli Salmonella Vacuna Sintética Synthetic Vaccines Ramírez, Hernán Vilte, Daniel Alejandro Hozbor, Daniela Flavia Zurita, Eugenia Bottero, Daniela Casabonne, Maria Carolina Cataldi, Angel Adrian Wigdorovitz, Andres Larzabal, Mariano A Novel Vaccine for Bovine Diarrhea Complex Utilizing Recombinant Enterotoxigenic Escherichia coli and Salmonella Expressing Surface-Displayed Chimeric Antigens from Enterohemorrhagic Escherichia coli O157:H7 |
| title | A Novel Vaccine for Bovine Diarrhea Complex Utilizing Recombinant Enterotoxigenic Escherichia coli and Salmonella Expressing Surface-Displayed Chimeric Antigens from Enterohemorrhagic Escherichia coli O157:H7 |
| title_full | A Novel Vaccine for Bovine Diarrhea Complex Utilizing Recombinant Enterotoxigenic Escherichia coli and Salmonella Expressing Surface-Displayed Chimeric Antigens from Enterohemorrhagic Escherichia coli O157:H7 |
| title_fullStr | A Novel Vaccine for Bovine Diarrhea Complex Utilizing Recombinant Enterotoxigenic Escherichia coli and Salmonella Expressing Surface-Displayed Chimeric Antigens from Enterohemorrhagic Escherichia coli O157:H7 |
| title_full_unstemmed | A Novel Vaccine for Bovine Diarrhea Complex Utilizing Recombinant Enterotoxigenic Escherichia coli and Salmonella Expressing Surface-Displayed Chimeric Antigens from Enterohemorrhagic Escherichia coli O157:H7 |
| title_short | A Novel Vaccine for Bovine Diarrhea Complex Utilizing Recombinant Enterotoxigenic Escherichia coli and Salmonella Expressing Surface-Displayed Chimeric Antigens from Enterohemorrhagic Escherichia coli O157:H7 |
| title_sort | novel vaccine for bovine diarrhea complex utilizing recombinant enterotoxigenic escherichia coli and salmonella expressing surface displayed chimeric antigens from enterohemorrhagic escherichia coli o157 h7 |
| topic | Enfermedades de los Animales Animal Diseases Vacuna Vaccines Diarrea Viral Bovina Bovine Viral Diarrhoea Respuesta Inmunológica Immune Response Escherichia coli Salmonella Vacuna Sintética Synthetic Vaccines |
| url | http://hdl.handle.net/20.500.12123/21473 https://www.mdpi.com/2076-393X/13/2/124 https://doi.org/10.3390/vaccines13020124 |
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