Elucidating the Connection Between the Health-Promoting Properties of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides
The potential probiotic properties of Limosilactobacillus fermentum Lf2, an exopolysaccharide (EPS)-producing strain, were assessed in C57BL/6 mice. The aim of this work was to elucidate if these properties could be associated with the ability to produce EPSs. Mice were divided into three treatments...
| Main Authors: | , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
MDPI
2025
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/21470 https://www.mdpi.com/2311-5637/11/2/69 https://doi.org/10.3390/fermentation11020069 |
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| author | Ale, Elisa C. Ale, Analia Peralta, Guillermo H. Irazoqui, Jose Matias Correa Olivar, Gabriela Allende Roldán, Victoria Vinderola, Celso Gabriel Amadio, Ariel Bergamini, Carina Viviana Cazenave, Jimena Binetti, Ana |
| author_browse | Ale, Analia Ale, Elisa C. Allende Roldán, Victoria Amadio, Ariel Bergamini, Carina Viviana Binetti, Ana Cazenave, Jimena Correa Olivar, Gabriela Irazoqui, Jose Matias Peralta, Guillermo H. Vinderola, Celso Gabriel |
| author_facet | Ale, Elisa C. Ale, Analia Peralta, Guillermo H. Irazoqui, Jose Matias Correa Olivar, Gabriela Allende Roldán, Victoria Vinderola, Celso Gabriel Amadio, Ariel Bergamini, Carina Viviana Cazenave, Jimena Binetti, Ana |
| author_sort | Ale, Elisa C. |
| collection | INTA Digital |
| description | The potential probiotic properties of Limosilactobacillus fermentum Lf2, an exopolysaccharide (EPS)-producing strain, were assessed in C57BL/6 mice. The aim of this work was to elucidate if these properties could be associated with the ability to produce EPSs. Mice were divided into three treatments: L (mice treated with Lf2), E (animals that received EPSs), and C (control group). The levels of fecal acetic and propionic acids significantly increased in L and E compared with C. Catalase activity increased in L in comparison with the other groups in the liver and small intestine. The enzyme activities of superoxide dismutase and glutathione S-transferase increased in the large intestine for L compared with C. In addition, in the large intestine, the concentration of TNF-α was reduced in L and E in comparison with C. In the small intestine, TNF-α, IFN-γ, IL-12, and IL-6 presented lower levels in L and E than C. The analysis of the gut microbiota showed that L presented higher levels of Peptococcaceae and Rikenellaceae, while E had higher levels of Peptococcaceae than C. Overall, these results provide new insights into the relationship between the probiotic properties of lactic acid bacteria and their ability to produce EPSs. |
| format | Artículo |
| id | INTA21470 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA214702025-02-26T12:01:22Z Elucidating the Connection Between the Health-Promoting Properties of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides Ale, Elisa C. Ale, Analia Peralta, Guillermo H. Irazoqui, Jose Matias Correa Olivar, Gabriela Allende Roldán, Victoria Vinderola, Celso Gabriel Amadio, Ariel Bergamini, Carina Viviana Cazenave, Jimena Binetti, Ana Lactobacillus Exopolysaccharides Lactic Acid Bacteria Oxidative Stress Immunomodulation Probiotics Exopolisacáridos Bacteria Acidoláctica Estrés Oxidativo Inmunomodulación Probióticos Limosilactobacillus fermentum The potential probiotic properties of Limosilactobacillus fermentum Lf2, an exopolysaccharide (EPS)-producing strain, were assessed in C57BL/6 mice. The aim of this work was to elucidate if these properties could be associated with the ability to produce EPSs. Mice were divided into three treatments: L (mice treated with Lf2), E (animals that received EPSs), and C (control group). The levels of fecal acetic and propionic acids significantly increased in L and E compared with C. Catalase activity increased in L in comparison with the other groups in the liver and small intestine. The enzyme activities of superoxide dismutase and glutathione S-transferase increased in the large intestine for L compared with C. In addition, in the large intestine, the concentration of TNF-α was reduced in L and E in comparison with C. In the small intestine, TNF-α, IFN-γ, IL-12, and IL-6 presented lower levels in L and E than C. The analysis of the gut microbiota showed that L presented higher levels of Peptococcaceae and Rikenellaceae, while E had higher levels of Peptococcaceae than C. Overall, these results provide new insights into the relationship between the probiotic properties of lactic acid bacteria and their ability to produce EPSs. EEA Rafaela Fil: Ale, E.C. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial; Argentina Fil: Ale, E.C. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Ale, Analía. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Department of Toxicology, Pharmacology and Legal Biochemistry; Argentina Fil: Ale, Analía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Peralta, Guillermo H. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial; Argentina Fil: Peralta, Guillermo H. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Correa Olivar, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial; Argentina Fil: Correa Olivar, Gabriela. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Allende Roldán, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial; Argentina Fil: Allende Roldán, Victoria. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; Argentina Fil: Vinderola, Celso Gabriel. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Amadio, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Bergamini, Carina Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial; Argentina Fil: Bergamini, Carina Viviana. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Nacional de Limnología; Argentina Fil: Cazenave, Jimena. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina Fil: Binetti, A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial; Argentina Fil: Binetti, Ana. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina 2025-02-26T11:59:17Z 2025-02-26T11:59:17Z 2025-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21470 https://www.mdpi.com/2311-5637/11/2/69 2311-5637 https://doi.org/10.3390/fermentation11020069 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Fermentation 11 (2) : 69. (February 2025) |
| spellingShingle | Lactobacillus Exopolysaccharides Lactic Acid Bacteria Oxidative Stress Immunomodulation Probiotics Exopolisacáridos Bacteria Acidoláctica Estrés Oxidativo Inmunomodulación Probióticos Limosilactobacillus fermentum Ale, Elisa C. Ale, Analia Peralta, Guillermo H. Irazoqui, Jose Matias Correa Olivar, Gabriela Allende Roldán, Victoria Vinderola, Celso Gabriel Amadio, Ariel Bergamini, Carina Viviana Cazenave, Jimena Binetti, Ana Elucidating the Connection Between the Health-Promoting Properties of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides |
| title | Elucidating the Connection Between the Health-Promoting Properties of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides |
| title_full | Elucidating the Connection Between the Health-Promoting Properties of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides |
| title_fullStr | Elucidating the Connection Between the Health-Promoting Properties of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides |
| title_full_unstemmed | Elucidating the Connection Between the Health-Promoting Properties of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides |
| title_short | Elucidating the Connection Between the Health-Promoting Properties of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides |
| title_sort | elucidating the connection between the health promoting properties of limosilactobacillus fermentum lf2 and its exopolysaccharides |
| topic | Lactobacillus Exopolysaccharides Lactic Acid Bacteria Oxidative Stress Immunomodulation Probiotics Exopolisacáridos Bacteria Acidoláctica Estrés Oxidativo Inmunomodulación Probióticos Limosilactobacillus fermentum |
| url | http://hdl.handle.net/20.500.12123/21470 https://www.mdpi.com/2311-5637/11/2/69 https://doi.org/10.3390/fermentation11020069 |
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