Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of Brucella abortus-bovine infected fetuses
Lectin binding relies on the affinity of these substances for specific terminal sugars. The method facilitates the identification of complex structures to which the terminal sugar attaches and may reveal physiological or pathological changes in cells, intracellular interactions or extracellular tr...
| Autores principales: | , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/2140 http://www.openveterinaryjournal.com/OVJ-2017-08-144%20M.A.%20Fiorentino%20et%20al.pdf http://dx.doi.org/10.4314/ovj.v8i1.10 |
| _version_ | 1855034911234195456 |
|---|---|
| author | Fiorentino, María Andrea Campero, Carlos Manuel Paolicchi, Fernando Barbeito, Claudio Gustavo |
| author_browse | Barbeito, Claudio Gustavo Campero, Carlos Manuel Fiorentino, María Andrea Paolicchi, Fernando |
| author_facet | Fiorentino, María Andrea Campero, Carlos Manuel Paolicchi, Fernando Barbeito, Claudio Gustavo |
| author_sort | Fiorentino, María Andrea |
| collection | INTA Digital |
| description | Lectin binding relies on the affinity of these substances for specific terminal sugars. The method facilitates the
identification of complex structures to which the terminal sugar attaches and may reveal physiological or pathological
changes in cells, intracellular interactions or extracellular transport pathways. This study was carried out to investigate
the effect of infection with Brucella abortus on the pattern of lectin binding in bovine fetal lungs (n=6) and bovine
placentas (n=5). Fetal lungs and placenta from heifers experimentally inoculated with B. abortus, strain 2308 were
examined by histological, lectin-histochemical, immunohistochemical and cultural techniques. B. abortus antigens
were immunohistochemically detected in fetal lungs and placenta. An increase in the labeling with UEA-1, DBA,
PNA, RCA-1 and SBA was found in the lungs and an increase in the labeling with UEA-1, ConA, PNA, DBA was
found in the placentas. The present lectin histochemical study revealed a distinctive pattern of oligosaccharide
distribution in the lungs and placenta of B. abortus-infected fetuses |
| format | info:ar-repo/semantics/artículo |
| id | INTA2140 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA21402018-03-27T18:47:06Z Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of Brucella abortus-bovine infected fetuses Fiorentino, María Andrea Campero, Carlos Manuel Paolicchi, Fernando Barbeito, Claudio Gustavo Brucella Abortus Ganado Bovino Lectinas Antígenos Placenta Pulmones Feto Técnicas Inmunológicas Infección Bovina Infection Immunological Techniques Foetus Lungs Antigens Lectins Cattle Lectin binding relies on the affinity of these substances for specific terminal sugars. The method facilitates the identification of complex structures to which the terminal sugar attaches and may reveal physiological or pathological changes in cells, intracellular interactions or extracellular transport pathways. This study was carried out to investigate the effect of infection with Brucella abortus on the pattern of lectin binding in bovine fetal lungs (n=6) and bovine placentas (n=5). Fetal lungs and placenta from heifers experimentally inoculated with B. abortus, strain 2308 were examined by histological, lectin-histochemical, immunohistochemical and cultural techniques. B. abortus antigens were immunohistochemically detected in fetal lungs and placenta. An increase in the labeling with UEA-1, DBA, PNA, RCA-1 and SBA was found in the lungs and an increase in the labeling with UEA-1, ConA, PNA, DBA was found in the placentas. The present lectin histochemical study revealed a distinctive pattern of oligosaccharide distribution in the lungs and placenta of B. abortus-infected fetuses Fil: Fiorentino, María Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Paolicchi, Fernando Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología e Instituto de Patología; Argentina 2018-03-27T18:30:44Z 2018-03-27T18:30:44Z 2018 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2140 http://www.openveterinaryjournal.com/OVJ-2017-08-144%20M.A.%20Fiorentino%20et%20al.pdf 2226-4485 2218-6050 (Online) http://dx.doi.org/10.4314/ovj.v8i1.10 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Open veterinary journal 8 (1), 57-63. (2018) |
| spellingShingle | Brucella Abortus Ganado Bovino Lectinas Antígenos Placenta Pulmones Feto Técnicas Inmunológicas Infección Bovina Infection Immunological Techniques Foetus Lungs Antigens Lectins Cattle Fiorentino, María Andrea Campero, Carlos Manuel Paolicchi, Fernando Barbeito, Claudio Gustavo Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of Brucella abortus-bovine infected fetuses |
| title | Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of Brucella abortus-bovine infected fetuses |
| title_full | Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of Brucella abortus-bovine infected fetuses |
| title_fullStr | Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of Brucella abortus-bovine infected fetuses |
| title_full_unstemmed | Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of Brucella abortus-bovine infected fetuses |
| title_short | Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of Brucella abortus-bovine infected fetuses |
| title_sort | lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of brucella abortus bovine infected fetuses |
| topic | Brucella Abortus Ganado Bovino Lectinas Antígenos Placenta Pulmones Feto Técnicas Inmunológicas Infección Bovina Infection Immunological Techniques Foetus Lungs Antigens Lectins Cattle |
| url | http://hdl.handle.net/20.500.12123/2140 http://www.openveterinaryjournal.com/OVJ-2017-08-144%20M.A.%20Fiorentino%20et%20al.pdf http://dx.doi.org/10.4314/ovj.v8i1.10 |
| work_keys_str_mv | AT fiorentinomariaandrea lectinbindingpatternsandimmunohistochemicalantigendetectioninplacentaandlungsofbrucellaabortusbovineinfectedfetuses AT camperocarlosmanuel lectinbindingpatternsandimmunohistochemicalantigendetectioninplacentaandlungsofbrucellaabortusbovineinfectedfetuses AT paolicchifernando lectinbindingpatternsandimmunohistochemicalantigendetectioninplacentaandlungsofbrucellaabortusbovineinfectedfetuses AT barbeitoclaudiogustavo lectinbindingpatternsandimmunohistochemicalantigendetectioninplacentaandlungsofbrucellaabortusbovineinfectedfetuses |