Identification, Sequencing and Genetic Diversity of Zucchini Tigre Mosaic Virus Infecting Cucurbita Species in Argentina
Cucurbit crops are constantly threatened by viral diseases. In Argentina, five potyviruses have been identified, including the recently discovered zucchini tigre mosaic virus (ZTMV). This study aimed to evaluate the molecular epidemiology of ZTMV. The virus was identified and characterised using mol...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/21381 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.14068 https://doi.org/10.1111/ppa.14068 |
| _version_ | 1855486744825167872 |
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| author | Brugo Carivali, María Florencia Luciani, Cecilia Elizabeth Celli, Marcos Giovani Olmos, Sofía Eugenia Obregon, Veronica Gabriela Silva, Milagros Itatí Fernandez, Franco Daniel Cabrera Mederos, Dariel Perotto, Maria Cecilia |
| author_browse | Brugo Carivali, María Florencia Cabrera Mederos, Dariel Celli, Marcos Giovani Fernandez, Franco Daniel Luciani, Cecilia Elizabeth Obregon, Veronica Gabriela Olmos, Sofía Eugenia Perotto, Maria Cecilia Silva, Milagros Itatí |
| author_facet | Brugo Carivali, María Florencia Luciani, Cecilia Elizabeth Celli, Marcos Giovani Olmos, Sofía Eugenia Obregon, Veronica Gabriela Silva, Milagros Itatí Fernandez, Franco Daniel Cabrera Mederos, Dariel Perotto, Maria Cecilia |
| author_sort | Brugo Carivali, María Florencia |
| collection | INTA Digital |
| description | Cucurbit crops are constantly threatened by viral diseases. In Argentina, five potyviruses have been identified, including the recently discovered zucchini tigre mosaic virus (ZTMV). This study aimed to evaluate the molecular epidemiology of ZTMV. The virus was identified and characterised using molecular techniques including reverse transcription-PCR, Sanger sequencing and Oxford Nanopore sequencing. Eighteen coat protein (CP) sequences and one complete genome sequence were obtained. Phylogenetically, the new Argentine isolates formed their own clade separated from all other ZTMV isolates. Population genetic analyses of CP sequences revealed high haplotype diversity and moderate-to-high nucleotide diversity across phylogroups. This region was under a strong negative selection. Neutrality tests were not statistically significant. The genetic differentiation parameters were statistically significant, indicating that ZTMV phylogenetic groups were different and supporting the proposed novel structure. Moreover, an infrequent gene flow between populations was observed. Haplotype analysis of 63 CP sequences revealed 54 distinct haplotypes, 15 of which originated from Argentina with a high number of mutations between clusters. The network grouped the haplotypes in the same clusters as those observed in the phylogenetic tree. One event of intraspecific recombination was found, which encompassed P1 and HC-Pro coding regions. The results obtained suggest that Argentine ZTMV populations have developed an evolutionary strategy characterised by low genetic diversity, low mutation rates and intraspecific recombination. All these strategies contribute to the virus adaptation to survival in new environments and hosts. |
| format | Artículo |
| id | INTA21381 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA213812025-02-21T11:01:39Z Identification, Sequencing and Genetic Diversity of Zucchini Tigre Mosaic Virus Infecting Cucurbita Species in Argentina Brugo Carivali, María Florencia Luciani, Cecilia Elizabeth Celli, Marcos Giovani Olmos, Sofía Eugenia Obregon, Veronica Gabriela Silva, Milagros Itatí Fernandez, Franco Daniel Cabrera Mederos, Dariel Perotto, Maria Cecilia Cucurbita Plant Viruses Potyvirus Identification Genetic Diversity (resource) Nanopore Sequencing Virus de las Plantas Identificación Diversidad Genética (recurso) Secuenciación por Nanoporos Argentina Zucchini Tigre Mosaic Virus Cucurbit crops are constantly threatened by viral diseases. In Argentina, five potyviruses have been identified, including the recently discovered zucchini tigre mosaic virus (ZTMV). This study aimed to evaluate the molecular epidemiology of ZTMV. The virus was identified and characterised using molecular techniques including reverse transcription-PCR, Sanger sequencing and Oxford Nanopore sequencing. Eighteen coat protein (CP) sequences and one complete genome sequence were obtained. Phylogenetically, the new Argentine isolates formed their own clade separated from all other ZTMV isolates. Population genetic analyses of CP sequences revealed high haplotype diversity and moderate-to-high nucleotide diversity across phylogroups. This region was under a strong negative selection. Neutrality tests were not statistically significant. The genetic differentiation parameters were statistically significant, indicating that ZTMV phylogenetic groups were different and supporting the proposed novel structure. Moreover, an infrequent gene flow between populations was observed. Haplotype analysis of 63 CP sequences revealed 54 distinct haplotypes, 15 of which originated from Argentina with a high number of mutations between clusters. The network grouped the haplotypes in the same clusters as those observed in the phylogenetic tree. One event of intraspecific recombination was found, which encompassed P1 and HC-Pro coding regions. The results obtained suggest that Argentine ZTMV populations have developed an evolutionary strategy characterised by low genetic diversity, low mutation rates and intraspecific recombination. All these strategies contribute to the virus adaptation to survival in new environments and hosts. Instituto de Patología Vegetal Fil: Brugo Carivali, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Brugo Carivali, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Luciani, Cecilia Elizabeth.Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Luciani, Cecilia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Celli, Marcos Giovani. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Olmos, Sofía Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benítez; Argentina Fil: Olmos, Sofía Eugenia. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Departamento de Producción Vegetal. Cátedra de Horticultura; Argentina Fil: Obregón, Verónica Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentina Fil: Silva, Milagros Itatí. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Silva, Milagros Itatí. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Fernandez, Franco Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Cabrera Mederos, Dariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Perotto, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2025-02-21T10:56:59Z 2025-02-21T10:56:59Z 2025-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21381 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.14068 0032-0862 1365-3059 https://doi.org/10.1111/ppa.14068 eng info:eu-repograntAgreement/INTA/2023-PD-L01-I074, Bases ecológicas y epidemiológicas para el diseño de estrategias de manejo de plagas agrícolas y forestales info:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejo info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Plant Pathology : 1-13. (First published: 14 February 2025) |
| spellingShingle | Cucurbita Plant Viruses Potyvirus Identification Genetic Diversity (resource) Nanopore Sequencing Virus de las Plantas Identificación Diversidad Genética (recurso) Secuenciación por Nanoporos Argentina Zucchini Tigre Mosaic Virus Brugo Carivali, María Florencia Luciani, Cecilia Elizabeth Celli, Marcos Giovani Olmos, Sofía Eugenia Obregon, Veronica Gabriela Silva, Milagros Itatí Fernandez, Franco Daniel Cabrera Mederos, Dariel Perotto, Maria Cecilia Identification, Sequencing and Genetic Diversity of Zucchini Tigre Mosaic Virus Infecting Cucurbita Species in Argentina |
| title | Identification, Sequencing and Genetic Diversity of Zucchini Tigre Mosaic Virus Infecting Cucurbita Species in Argentina |
| title_full | Identification, Sequencing and Genetic Diversity of Zucchini Tigre Mosaic Virus Infecting Cucurbita Species in Argentina |
| title_fullStr | Identification, Sequencing and Genetic Diversity of Zucchini Tigre Mosaic Virus Infecting Cucurbita Species in Argentina |
| title_full_unstemmed | Identification, Sequencing and Genetic Diversity of Zucchini Tigre Mosaic Virus Infecting Cucurbita Species in Argentina |
| title_short | Identification, Sequencing and Genetic Diversity of Zucchini Tigre Mosaic Virus Infecting Cucurbita Species in Argentina |
| title_sort | identification sequencing and genetic diversity of zucchini tigre mosaic virus infecting cucurbita species in argentina |
| topic | Cucurbita Plant Viruses Potyvirus Identification Genetic Diversity (resource) Nanopore Sequencing Virus de las Plantas Identificación Diversidad Genética (recurso) Secuenciación por Nanoporos Argentina Zucchini Tigre Mosaic Virus |
| url | http://hdl.handle.net/20.500.12123/21381 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.14068 https://doi.org/10.1111/ppa.14068 |
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