Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from Argentina
Field captured specimens of Didelphis albiventris (Didelphimorphia: Didelphidae) from Formosa Province, Argentina, were analysed for possible infection with Babesia species. Whole blood samples of live-trapped D. albiventris were taken, genomic DNA was extracted and a Piroplasmida specific PCR assay...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Elsevier
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/21367 https://www.sciencedirect.com/science/article/abs/pii/S2405939025000127 https://doi.org/10.1016/j.vprsr.2025.101205 |
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| author | Sebastian, Patrick Lamattina, Daniela Vaschalde, Paula Josefina Flores, Fernando Sebastián Torrents, Jorgelina Tarragona, Evelina Luisa |
| author_browse | Flores, Fernando Sebastián Lamattina, Daniela Sebastian, Patrick Tarragona, Evelina Luisa Torrents, Jorgelina Vaschalde, Paula Josefina |
| author_facet | Sebastian, Patrick Lamattina, Daniela Vaschalde, Paula Josefina Flores, Fernando Sebastián Torrents, Jorgelina Tarragona, Evelina Luisa |
| author_sort | Sebastian, Patrick |
| collection | INTA Digital |
| description | Field captured specimens of Didelphis albiventris (Didelphimorphia: Didelphidae) from Formosa Province, Argentina, were analysed for possible infection with Babesia species. Whole blood samples of live-trapped D. albiventris were taken, genomic DNA was extracted and a Piroplasmida specific PCR assay targeting a fragment of the 18S rRNA gene was conducted. Positive PCR amplicon were sequenced and obtained DNA partial sequences were located in a phylogenetic context by constructing a maximum-likelihood tree. Two samples were positive and could be identified as Babesia species. Through phylogenetic analyses, both sequences were demonstrated to belong to a group of Babesia sp. associated with marsupials from Brazil and therefore previously defined as the “South American Marsupialia Group” (SAMG) of Babesia species. The results of this study represent the first report of a Piroplasmida species in a marsupial in Argentina. They further give an insight on the circulation of Babesia sp. in D. albiventris that, due to its synanthropic habits, plays an epidemiologically important role as potential hosts for emerging pathogens and ectoparasites of relevance in veterinary medicine. |
| format | Artículo |
| id | INTA21367 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA213672025-09-15T10:41:39Z Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from Argentina Sebastian, Patrick Lamattina, Daniela Vaschalde, Paula Josefina Flores, Fernando Sebastián Torrents, Jorgelina Tarragona, Evelina Luisa Babesia Didelphis Identification Ticks Identificación Garrapata Argentina Didelphis albiventris Field captured specimens of Didelphis albiventris (Didelphimorphia: Didelphidae) from Formosa Province, Argentina, were analysed for possible infection with Babesia species. Whole blood samples of live-trapped D. albiventris were taken, genomic DNA was extracted and a Piroplasmida specific PCR assay targeting a fragment of the 18S rRNA gene was conducted. Positive PCR amplicon were sequenced and obtained DNA partial sequences were located in a phylogenetic context by constructing a maximum-likelihood tree. Two samples were positive and could be identified as Babesia species. Through phylogenetic analyses, both sequences were demonstrated to belong to a group of Babesia sp. associated with marsupials from Brazil and therefore previously defined as the “South American Marsupialia Group” (SAMG) of Babesia species. The results of this study represent the first report of a Piroplasmida species in a marsupial in Argentina. They further give an insight on the circulation of Babesia sp. in D. albiventris that, due to its synanthropic habits, plays an epidemiologically important role as potential hosts for emerging pathogens and ectoparasites of relevance in veterinary medicine. EEA Rafaela Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Lamattina, Daniela. Instituto Nacional de Medicina Tropical (Misiones); Argentina Fil: Vaschalde, Paula J. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; Argentina Fil: Vaschalde, Paula J. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; Argentina Fil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba (CIEC); Argentina Fil: Flores, Fernando Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina Fil: Torrents, Jorgelina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Tarragona, Evelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Tarragona, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina 2025-02-20T11:39:42Z 2025-02-20T11:39:42Z 2025-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21367 https://www.sciencedirect.com/science/article/abs/pii/S2405939025000127 2405-9390 https://doi.org/10.1016/j.vprsr.2025.101205 eng info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001, Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Veterinary Parasitology: Regional Studies and Reports 58 : 101205. (February 2025) |
| spellingShingle | Babesia Didelphis Identification Ticks Identificación Garrapata Argentina Didelphis albiventris Sebastian, Patrick Lamattina, Daniela Vaschalde, Paula Josefina Flores, Fernando Sebastián Torrents, Jorgelina Tarragona, Evelina Luisa Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from Argentina |
| title | Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from Argentina |
| title_full | Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from Argentina |
| title_fullStr | Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from Argentina |
| title_full_unstemmed | Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from Argentina |
| title_short | Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from Argentina |
| title_sort | babesia sp of the south american marsupialia group first molecular detection in didelphis albiventris didelphimorphia didelphidae from argentina |
| topic | Babesia Didelphis Identification Ticks Identificación Garrapata Argentina Didelphis albiventris |
| url | http://hdl.handle.net/20.500.12123/21367 https://www.sciencedirect.com/science/article/abs/pii/S2405939025000127 https://doi.org/10.1016/j.vprsr.2025.101205 |
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