The Role of Reproductive Modes in Shaping Genetic Diversity in Polyploids: A Comparative Study of Selfing, Outcrossing, and Apomictic Paspalum Species

Exploring the genetic diversity and reproductive strategies of Paspalum species is essential for advancing forage grass improvement. We compared morpho-phenological, molecular, and genotypic variation in five tetraploid Paspalum species with contrasting mating systems and reproductive modes. Contrar...

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Main Authors: Reutemann, Verena, Schedler, Mara, Hojsgaard, Diego H., Brugnoli, Elsa Andrea, Zilli, Alex Leonel, Acuña, Carlos Alberto, Honfi, Ana I., Martínez, Eric Javier
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: MDPI 2025
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/21363
https://www.mdpi.com/2223-7747/14/3/476
https://doi.org/10.3390/plants14030476
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author Reutemann, Verena
Schedler, Mara
Hojsgaard, Diego H.
Brugnoli, Elsa Andrea
Zilli, Alex Leonel
Acuña, Carlos Alberto
Honfi, Ana I.
Martínez, Eric Javier
author_browse Acuña, Carlos Alberto
Brugnoli, Elsa Andrea
Hojsgaard, Diego H.
Honfi, Ana I.
Martínez, Eric Javier
Reutemann, Verena
Schedler, Mara
Zilli, Alex Leonel
author_facet Reutemann, Verena
Schedler, Mara
Hojsgaard, Diego H.
Brugnoli, Elsa Andrea
Zilli, Alex Leonel
Acuña, Carlos Alberto
Honfi, Ana I.
Martínez, Eric Javier
author_sort Reutemann, Verena
collection INTA Digital
description Exploring the genetic diversity and reproductive strategies of Paspalum species is essential for advancing forage grass improvement. We compared morpho-phenological, molecular, and genotypic variation in five tetraploid Paspalum species with contrasting mating systems and reproductive modes. Contrary to previous findings, selfing (Paspalum regnellii and P. urvillei) and outcrossing (P. durifolium and P. ionanthum) species exhibited similar phenotypic diversity patterns, with low intrapopulation variability and no morphological differentiation among populations. The apomictic species (P. intermedium) exhibited low intrapopulation phenotypic variation but high population differentiation, indicative of genetic drift and local adaptation. Outcrossing species showed greater intrapopulation genotypic variation than selfing species, which displayed a high population structure due to restricted pollen migration. The apomictic species exhibited the lowest intrapopulation molecular diversity, forming uniclonal populations with high interpopulation differentiation, highlighting the fixation of distinct gene pools via apomixis. This is the first report about genetic diversity in populations of sexual allopolyploid species of Paspalum. Population structure in these allotetraploid Paspalum species is primarily shaped by how reproductive modes, mating systems, and geographic distribution influence gene flow via pollen and seeds. Our findings contribute significantly to the conservation and genetic improvement of forage grasses, particularly for developing cultivars with enhanced adaptability and productivity.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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spelling INTA213632025-02-20T10:41:41Z The Role of Reproductive Modes in Shaping Genetic Diversity in Polyploids: A Comparative Study of Selfing, Outcrossing, and Apomictic Paspalum Species Reutemann, Verena Schedler, Mara Hojsgaard, Diego H. Brugnoli, Elsa Andrea Zilli, Alex Leonel Acuña, Carlos Alberto Honfi, Ana I. Martínez, Eric Javier Paspalum Variación Genética Apomixis Cruzamiento Estructura de la Población Autofecundación Genetic Variation Cross-breeding Population Structure Selfing Exploring the genetic diversity and reproductive strategies of Paspalum species is essential for advancing forage grass improvement. We compared morpho-phenological, molecular, and genotypic variation in five tetraploid Paspalum species with contrasting mating systems and reproductive modes. Contrary to previous findings, selfing (Paspalum regnellii and P. urvillei) and outcrossing (P. durifolium and P. ionanthum) species exhibited similar phenotypic diversity patterns, with low intrapopulation variability and no morphological differentiation among populations. The apomictic species (P. intermedium) exhibited low intrapopulation phenotypic variation but high population differentiation, indicative of genetic drift and local adaptation. Outcrossing species showed greater intrapopulation genotypic variation than selfing species, which displayed a high population structure due to restricted pollen migration. The apomictic species exhibited the lowest intrapopulation molecular diversity, forming uniclonal populations with high interpopulation differentiation, highlighting the fixation of distinct gene pools via apomixis. This is the first report about genetic diversity in populations of sexual allopolyploid species of Paspalum. Population structure in these allotetraploid Paspalum species is primarily shaped by how reproductive modes, mating systems, and geographic distribution influence gene flow via pollen and seeds. Our findings contribute significantly to the conservation and genetic improvement of forage grasses, particularly for developing cultivars with enhanced adaptability and productivity. EEA Montecarlo Fil: Reutemann, Verena. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Grupo de Genética y Mejoramiento de Especies Forrajeras; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Grupo de Genética y Mejoramiento de Especies Forrajeras; Argentina Fil: Schedler, Mara. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentina Fil: Hojsgaard, Diego. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK); Alemania Fil: Brugnoli, Elsa. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Grupo de Genética y Mejoramiento de Especies Forrajeras; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Grupo de Genética y Mejoramiento de Especies Forrajeras; Argentina Fil: Acuña, Carlos. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Grupo de Genética y Mejoramiento de Especies Forrajeras; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Grupo de Genética y Mejoramiento de Especies Forrajeras; Argentina Fil: Honfi, Ana. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biología Subtropical. Programa de Estudios Florísticos y Genética Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Subtropical. Programa de Estudios Florísticos y Genética Vegetal; Argentina Fil: Martínez, Eric Javier. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Grupo de Genética y Mejoramiento de Especies Forrajeras; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Grupo de Genética y Mejoramiento de Especies Forrajeras; Argentina 2025-02-20T10:23:33Z 2025-02-20T10:23:33Z 2025-02-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21363 https://www.mdpi.com/2223-7747/14/3/476 Reutemann, A. V., Schedler, M., Hojsgaard, D. H., Brugnoli, E. A., Zilli, A. L., Acuña, C. A., Honfi, A. I., & Martínez, E. J. (2025). The Role of Reproductive Modes in Shaping Genetic Diversity in Polyploids: A Comparative Study of Selfing, Outcrossing, and Apomictic Paspalum Species. Plants, 14(3), 476. https://doi.org/10.3390/plants14030476 2223-7747 https://doi.org/10.3390/plants14030476 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Plants 14 (3) : 476. (February 2025)
spellingShingle Paspalum
Variación Genética
Apomixis
Cruzamiento
Estructura de la Población
Autofecundación
Genetic Variation
Cross-breeding
Population Structure
Selfing
Reutemann, Verena
Schedler, Mara
Hojsgaard, Diego H.
Brugnoli, Elsa Andrea
Zilli, Alex Leonel
Acuña, Carlos Alberto
Honfi, Ana I.
Martínez, Eric Javier
The Role of Reproductive Modes in Shaping Genetic Diversity in Polyploids: A Comparative Study of Selfing, Outcrossing, and Apomictic Paspalum Species
title The Role of Reproductive Modes in Shaping Genetic Diversity in Polyploids: A Comparative Study of Selfing, Outcrossing, and Apomictic Paspalum Species
title_full The Role of Reproductive Modes in Shaping Genetic Diversity in Polyploids: A Comparative Study of Selfing, Outcrossing, and Apomictic Paspalum Species
title_fullStr The Role of Reproductive Modes in Shaping Genetic Diversity in Polyploids: A Comparative Study of Selfing, Outcrossing, and Apomictic Paspalum Species
title_full_unstemmed The Role of Reproductive Modes in Shaping Genetic Diversity in Polyploids: A Comparative Study of Selfing, Outcrossing, and Apomictic Paspalum Species
title_short The Role of Reproductive Modes in Shaping Genetic Diversity in Polyploids: A Comparative Study of Selfing, Outcrossing, and Apomictic Paspalum Species
title_sort role of reproductive modes in shaping genetic diversity in polyploids a comparative study of selfing outcrossing and apomictic paspalum species
topic Paspalum
Variación Genética
Apomixis
Cruzamiento
Estructura de la Población
Autofecundación
Genetic Variation
Cross-breeding
Population Structure
Selfing
url http://hdl.handle.net/20.500.12123/21363
https://www.mdpi.com/2223-7747/14/3/476
https://doi.org/10.3390/plants14030476
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