Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixture
BACKGROUND: Glyphosate-resistant populations of some grass species have demonstrated high sensitivity to combinations of glyphosate and acetyl-CoA carboxylase (ACCase)-inhibiting herbicides. Nevertheless, it is unclear how glyphosate contributes to the control of glyphosate-resistant plants when com...
| Autores principales: | , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/21346 https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.8719 https://doi.org/10.1002/ps.8719 |
| _version_ | 1855038387978764288 |
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| author | Yanniccari, Marcos Gigon, Ramon |
| author_browse | Gigon, Ramon Yanniccari, Marcos |
| author_facet | Yanniccari, Marcos Gigon, Ramon |
| author_sort | Yanniccari, Marcos |
| collection | INTA Digital |
| description | BACKGROUND: Glyphosate-resistant populations of some grass species have demonstrated high sensitivity to combinations of glyphosate and acetyl-CoA carboxylase (ACCase)-inhibiting herbicides. Nevertheless, it is unclear how glyphosate contributes to the control of glyphosate-resistant plants when combined with other active ingredients such as clethodim. The current work aimed to detect if glyphosate-resistant Lolium perenne L. biotypes associated with target-site [5-enolpyruvylshikimate-3-phosphate synthase (EPSPS)] overexpression and EPSPS mutation (P106S) as mechanisms of resistance are equally controlled by clethodim or clethodim plus glyphosate compared to a susceptible counterpart.
RESULTS: Plants of the EPSPS overexpression biotype were most sensitive to glyphosate when it was combined with clethodim, and the dose required to halve the maximal response (I50) calculated for glyphosate was reduced to a third. However, the ACCase inhibitor seems to be uniquely responsible for weed control in the P106S biotype. In response to clethodim, EPSPS expression was inhibited in the glyphosate-resistant EPSPS overexpression biotype to a level like or lower than that of susceptible plants. The glyphosate plus clethodim mixture caused a 1.7-fold increase in shikimate concentration in the leaves of susceptible and EPSPS overexpression biotypes compared to glyphosate alone.
CONCLUSION: The effects of glyphosate plus clethodim impact in different senses according to the mechanism of resistance involved, where the convergent action of both herbicides controlled the susceptible and EPSPS overexpression biotypes. In contrast, the P106S biotype died because of the clethodim component in the mixture. Mixing herbicides should not be considered a complete solution to herbicide resistance management, and its potential seems dependent on the resistance mechanism involved. |
| format | info:ar-repo/semantics/artículo |
| id | INTA21346 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA213462025-02-19T11:35:10Z Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixture Yanniccari, Marcos Gigon, Ramon Lolium perenne Glyphosate Clethodim Herbicides Resistance to Herbicides Weeds Glifosato Cletodim Herbicidas Resistencia a los Herbicidas Malezas BACKGROUND: Glyphosate-resistant populations of some grass species have demonstrated high sensitivity to combinations of glyphosate and acetyl-CoA carboxylase (ACCase)-inhibiting herbicides. Nevertheless, it is unclear how glyphosate contributes to the control of glyphosate-resistant plants when combined with other active ingredients such as clethodim. The current work aimed to detect if glyphosate-resistant Lolium perenne L. biotypes associated with target-site [5-enolpyruvylshikimate-3-phosphate synthase (EPSPS)] overexpression and EPSPS mutation (P106S) as mechanisms of resistance are equally controlled by clethodim or clethodim plus glyphosate compared to a susceptible counterpart. RESULTS: Plants of the EPSPS overexpression biotype were most sensitive to glyphosate when it was combined with clethodim, and the dose required to halve the maximal response (I50) calculated for glyphosate was reduced to a third. However, the ACCase inhibitor seems to be uniquely responsible for weed control in the P106S biotype. In response to clethodim, EPSPS expression was inhibited in the glyphosate-resistant EPSPS overexpression biotype to a level like or lower than that of susceptible plants. The glyphosate plus clethodim mixture caused a 1.7-fold increase in shikimate concentration in the leaves of susceptible and EPSPS overexpression biotypes compared to glyphosate alone. CONCLUSION: The effects of glyphosate plus clethodim impact in different senses according to the mechanism of resistance involved, where the convergent action of both herbicides controlled the susceptible and EPSPS overexpression biotypes. In contrast, the P106S biotype died because of the clethodim component in the mixture. Mixing herbicides should not be considered a complete solution to herbicide resistance management, and its potential seems dependent on the resistance mechanism involved. EEA Barrow Fil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina Fil: Gigón, Ramón. Consultor privado en Control de Malezas; Argentina 2025-02-19T11:29:34Z 2025-02-19T11:29:34Z 2025-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21346 https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.8719 1526-498X 1526-4998 https://doi.org/10.1002/ps.8719 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Pest Management Science : 1-7 (First published: 12 February 2025) |
| spellingShingle | Lolium perenne Glyphosate Clethodim Herbicides Resistance to Herbicides Weeds Glifosato Cletodim Herbicidas Resistencia a los Herbicidas Malezas Yanniccari, Marcos Gigon, Ramon Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixture |
| title | Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixture |
| title_full | Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixture |
| title_fullStr | Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixture |
| title_full_unstemmed | Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixture |
| title_short | Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixture |
| title_sort | inhibition of epsps overexpression of glyphosate resistant lolium perenne l with a clethodim and glyphosate mixture |
| topic | Lolium perenne Glyphosate Clethodim Herbicides Resistance to Herbicides Weeds Glifosato Cletodim Herbicidas Resistencia a los Herbicidas Malezas |
| url | http://hdl.handle.net/20.500.12123/21346 https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.8719 https://doi.org/10.1002/ps.8719 |
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