Molecular, biological, and morphometric comparisons between different geographical populations of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae)
In this study, different geographical populations of Rhipicephalus sanguineus sensu lato were compared by molecular, biological, and morphometric methods. Phylogenetic trees were constructed using 12S and 16S rDNA sequences and showed two distinct clades: one composed of ticks from Brazil (Jaboticab...
| Main Authors: | , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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2018
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| Online Access: | https://www.sciencedirect.com/science/article/pii/S0304401715300741 http://ri.conicet.gov.ar/handle/11336/38211 http://hdl.handle.net/20.500.12123/2124 https://doi.org/10.1016/j.vetpar.2015.11.007 |
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| author | Sanches, Gustavo S. Évora, Patrícia M. Mangold, Atilio Jose Jittapalapong, Sattaporn Rodriguez Mallon, Alina Guzmán, Pedro E.E. Bechara, Gervásio H. Camargo Mathias, Maria I. |
| author_browse | Bechara, Gervásio H. Camargo Mathias, Maria I. Guzmán, Pedro E.E. Jittapalapong, Sattaporn Mangold, Atilio Jose Rodriguez Mallon, Alina Sanches, Gustavo S. Évora, Patrícia M. |
| author_facet | Sanches, Gustavo S. Évora, Patrícia M. Mangold, Atilio Jose Jittapalapong, Sattaporn Rodriguez Mallon, Alina Guzmán, Pedro E.E. Bechara, Gervásio H. Camargo Mathias, Maria I. |
| author_sort | Sanches, Gustavo S. |
| collection | INTA Digital |
| description | In this study, different geographical populations of Rhipicephalus sanguineus sensu lato were compared by molecular, biological, and morphometric methods. Phylogenetic trees were constructed using 12S and 16S rDNA sequences and showed two distinct clades: one composed of ticks from Brazil (Jaboticabal, SP), Cuba (Havana) Thailand (Bangkok) and the so-called “tropical strain” ticks. The second clade was composed of ticks from Spain (Zaragoza), Argentina (Rafaela, Santa Fe) and the so-called “temperate strain” ticks. Morphometric analysis showed good separation between females of the two clades and within the temperate clade. Males also exhibited separation between the two clades, but with some overlap. Multiple biological parameters revealed differences between the two clades, especially the weight of the engorged female. These results confirm the existence of at least two species under the name “R. sanguineus”. |
| format | Artículo |
| id | INTA2124 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA21242018-07-05T18:48:23Z Molecular, biological, and morphometric comparisons between different geographical populations of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) Sanches, Gustavo S. Évora, Patrícia M. Mangold, Atilio Jose Jittapalapong, Sattaporn Rodriguez Mallon, Alina Guzmán, Pedro E.E. Bechara, Gervásio H. Camargo Mathias, Maria I. Rhipicephalus Sanguineus Plagas de Animales ADN Análisis Biológico Pests of Animals DNA Biological Analysis Garrapatas In this study, different geographical populations of Rhipicephalus sanguineus sensu lato were compared by molecular, biological, and morphometric methods. Phylogenetic trees were constructed using 12S and 16S rDNA sequences and showed two distinct clades: one composed of ticks from Brazil (Jaboticabal, SP), Cuba (Havana) Thailand (Bangkok) and the so-called “tropical strain” ticks. The second clade was composed of ticks from Spain (Zaragoza), Argentina (Rafaela, Santa Fe) and the so-called “temperate strain” ticks. Morphometric analysis showed good separation between females of the two clades and within the temperate clade. Males also exhibited separation between the two clades, but with some overlap. Multiple biological parameters revealed differences between the two clades, especially the weight of the engorged female. These results confirm the existence of at least two species under the name “R. sanguineus”. EEA Rafaela Fil: Sanches, Gustavo S. Universidade de Sao Paulo. Departamento de Biologia. Instituto de Biociências; Brasil. Universidade de Sao Paulo. Departamento de Patologia Veterinária. Laboratório de Imunopatologia; Brasil Fil: Évora, Patrícia M. Universidade de Sao Paulo. Departamento de Patologia Veterinária. Laboratório de Imunopatologia; Brasil Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Jittapalapong, Sattaporn. Kasetsart University. Faculty of Veterinary Medicine; Tailandia Fil: Rodriguez Mallon, Alina. Centro de Ingeniería Genética y Biotecnología; Cuba Fil: Guzmán, Pedro E.E. Centro de Ingeniería Genética y Biotecnología; Cuba Fil: Bechara, Gervásio H. Universidade de Sao Paulo. Departamento de Patologia Veterinária. Laboratório de Imunopatologia; Brasil. Pontifícia Universidade Católica do Paraná; Brasil Fil: Camargo Mathias, Maria I. Universidade de Sao Paulo. Departamento de Biologia. Instituto de Biociências; Brasil. 2018-03-26T15:07:09Z 2018-03-26T15:07:09Z 2016-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0304401715300741 http://ri.conicet.gov.ar/handle/11336/38211 http://hdl.handle.net/20.500.12123/2124 0304-4017 https://doi.org/10.1016/j.vetpar.2015.11.007 eng info:eu-repo/semantics/restrictedAccess application/pdf Veterinary parasitology 215 : 78-87. (January 2016) |
| spellingShingle | Rhipicephalus Sanguineus Plagas de Animales ADN Análisis Biológico Pests of Animals DNA Biological Analysis Garrapatas Sanches, Gustavo S. Évora, Patrícia M. Mangold, Atilio Jose Jittapalapong, Sattaporn Rodriguez Mallon, Alina Guzmán, Pedro E.E. Bechara, Gervásio H. Camargo Mathias, Maria I. Molecular, biological, and morphometric comparisons between different geographical populations of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) |
| title | Molecular, biological, and morphometric comparisons between different geographical populations of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) |
| title_full | Molecular, biological, and morphometric comparisons between different geographical populations of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) |
| title_fullStr | Molecular, biological, and morphometric comparisons between different geographical populations of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) |
| title_full_unstemmed | Molecular, biological, and morphometric comparisons between different geographical populations of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) |
| title_short | Molecular, biological, and morphometric comparisons between different geographical populations of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) |
| title_sort | molecular biological and morphometric comparisons between different geographical populations of rhipicephalus sanguineus sensu lato acari ixodidae |
| topic | Rhipicephalus Sanguineus Plagas de Animales ADN Análisis Biológico Pests of Animals DNA Biological Analysis Garrapatas |
| url | https://www.sciencedirect.com/science/article/pii/S0304401715300741 http://ri.conicet.gov.ar/handle/11336/38211 http://hdl.handle.net/20.500.12123/2124 https://doi.org/10.1016/j.vetpar.2015.11.007 |
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