Autochthonous Lacticaseibacillus rhamnosus strains as probiotic candidates: comprehensive in vitro and in silico characterization
This study aimed to identify promising probiotic candidates among 25 autochthonous lactic acid bacteria (LAB) isolates from dairy products sourced from southeastern Brazil. In vitro assays were carried out to elucidate their phenotypic profiles, focusing on key probiotic attributes (antimicrobial, a...
| Main Authors: | , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/21132 https://www.sciencedirect.com/science/article/abs/pii/S2212429225022448 https://doi.org/10.1016/j.fbio.2025.108066 |
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| author | De Gregorio, Priscilla Romina Lieske, Isadora Nied, Cathy Signori, Gabriela Kuhn, Daniel Schmitz, Caroline Martins, Vera Lúcia Milani Bustamante Filho, Ivan Cunha Irazoqui, Jose Matias Souza, Claucia Fernanda Volken de |
| author_browse | Bustamante Filho, Ivan Cunha De Gregorio, Priscilla Romina Irazoqui, Jose Matias Kuhn, Daniel Lieske, Isadora Martins, Vera Lúcia Milani Nied, Cathy Schmitz, Caroline Signori, Gabriela Souza, Claucia Fernanda Volken de |
| author_facet | De Gregorio, Priscilla Romina Lieske, Isadora Nied, Cathy Signori, Gabriela Kuhn, Daniel Schmitz, Caroline Martins, Vera Lúcia Milani Bustamante Filho, Ivan Cunha Irazoqui, Jose Matias Souza, Claucia Fernanda Volken de |
| author_sort | De Gregorio, Priscilla Romina |
| collection | INTA Digital |
| description | This study aimed to identify promising probiotic candidates among 25 autochthonous lactic acid bacteria (LAB) isolates from dairy products sourced from southeastern Brazil. In vitro assays were carried out to elucidate their phenotypic profiles, focusing on key probiotic attributes (antimicrobial, antioxidant and β-galactosidase activities, resistance to simulated gastrointestinal conditions, antibiotic susceptibility, and adhesion capacity). Principal Component Analysis was subsequently employed to rank the most promising strains. Among the isolates, four Lacticaseibacillus rhamnosus strains (6QLR5, GTM7, CLM11, and JLR3) demonstrated promising antimicrobial activity, adhesion capacity, tolerance to gastrointestinal conditions, antioxidant capacity, and β-galactosidase activity. Of these, strains 6QLR5 and GTM7 displayed the most robust phenotypic probiotic profiles and were selected for in silico genomic analysis. Functional annotation revealed genes associated with key probiotic traits, including redox systems related to antioxidant activity, adhesion-associated proteins, and mechanisms of resistance and adaptation to the harsh conditions of the intestinal tract. Genomic safety assessment, encompassing antimicrobial resistance genes, mobile genetic elements, virulence factors, and genome-stability features, indicated that strains 6QLR5 and GTM7 are safe probiotic candidates, as they harbored no genes linked to antibiotic resistance, pathogenicity, or virulence, and lacked hazardous plasmids or prophages. Overall, the characterization of autochthonous LAB strains based on integrative in vitro and in silico approaches proved effective for selecting probiotic candidates. |
| format | Artículo |
| id | INTA21132 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA211322026-01-09T13:11:46Z Autochthonous Lacticaseibacillus rhamnosus strains as probiotic candidates: comprehensive in vitro and in silico characterization De Gregorio, Priscilla Romina Lieske, Isadora Nied, Cathy Signori, Gabriela Kuhn, Daniel Schmitz, Caroline Martins, Vera Lúcia Milani Bustamante Filho, Ivan Cunha Irazoqui, Jose Matias Souza, Claucia Fernanda Volken de Lactic Acid Bacteria Probiotics Genomics Milk Products Bacteria Acidoláctica Probióticos Genómica Experimentación in Vitro Productos Lácteos Lacticaseibacillus rhamnosus This study aimed to identify promising probiotic candidates among 25 autochthonous lactic acid bacteria (LAB) isolates from dairy products sourced from southeastern Brazil. In vitro assays were carried out to elucidate their phenotypic profiles, focusing on key probiotic attributes (antimicrobial, antioxidant and β-galactosidase activities, resistance to simulated gastrointestinal conditions, antibiotic susceptibility, and adhesion capacity). Principal Component Analysis was subsequently employed to rank the most promising strains. Among the isolates, four Lacticaseibacillus rhamnosus strains (6QLR5, GTM7, CLM11, and JLR3) demonstrated promising antimicrobial activity, adhesion capacity, tolerance to gastrointestinal conditions, antioxidant capacity, and β-galactosidase activity. Of these, strains 6QLR5 and GTM7 displayed the most robust phenotypic probiotic profiles and were selected for in silico genomic analysis. Functional annotation revealed genes associated with key probiotic traits, including redox systems related to antioxidant activity, adhesion-associated proteins, and mechanisms of resistance and adaptation to the harsh conditions of the intestinal tract. Genomic safety assessment, encompassing antimicrobial resistance genes, mobile genetic elements, virulence factors, and genome-stability features, indicated that strains 6QLR5 and GTM7 are safe probiotic candidates, as they harbored no genes linked to antibiotic resistance, pathogenicity, or virulence, and lacked hazardous plasmids or prophages. Overall, the characterization of autochthonous LAB strains based on integrative in vitro and in silico approaches proved effective for selecting probiotic candidates. EEA Rafaela Fil: De Gregorio, Priscilla Romina. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil Fil: De Gregorio, Priscilla Romina. University of Vale Do Taquari - Univates. Biotechnology Graduate Program; Brasil Fil: De Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Referencia para Lactobacilos (CERELA); Argentina Fil: Lieske, Isadora. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil Fil: Lieske, Isadora. University of Vale Do Taquari - Univates. Biotechnology Graduate Program; Brasil Fil: Nied, Cathy. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil Fil: Signori, Gabriela. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil Fil: Kuhn, Daniel. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil Fil: Schmitz, Caroline. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil Fil: Martins, Vera Lúcia Milani. Federal Institute of Education, Science and Technology of Rio Grande Do Sul; Brasil Fil: Bustamante Filho, Ivan Cunha. University of Vale Do Taquari - Univates. Biotechnology Graduate Program; Brasil Fil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Souza, Claucia Fernanda Volken de. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil Fil: Souza, Claucia Fernanda Volken de. University of Vale Do Taquari - Univates. Biotechnology Graduate Program; Brasil In Vitro Experimentation 2025-02-05T11:47:06Z 2025-02-05T11:47:06Z 2026-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21132 https://www.sciencedirect.com/science/article/abs/pii/S2212429225022448 2212-4292 2212-4306 https://doi.org/10.1016/j.fbio.2025.108066 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Food Bioscience 75 : 108066. (January 2026) |
| spellingShingle | Lactic Acid Bacteria Probiotics Genomics Milk Products Bacteria Acidoláctica Probióticos Genómica Experimentación in Vitro Productos Lácteos Lacticaseibacillus rhamnosus De Gregorio, Priscilla Romina Lieske, Isadora Nied, Cathy Signori, Gabriela Kuhn, Daniel Schmitz, Caroline Martins, Vera Lúcia Milani Bustamante Filho, Ivan Cunha Irazoqui, Jose Matias Souza, Claucia Fernanda Volken de Autochthonous Lacticaseibacillus rhamnosus strains as probiotic candidates: comprehensive in vitro and in silico characterization |
| title | Autochthonous Lacticaseibacillus rhamnosus strains as probiotic candidates: comprehensive in vitro and in silico characterization |
| title_full | Autochthonous Lacticaseibacillus rhamnosus strains as probiotic candidates: comprehensive in vitro and in silico characterization |
| title_fullStr | Autochthonous Lacticaseibacillus rhamnosus strains as probiotic candidates: comprehensive in vitro and in silico characterization |
| title_full_unstemmed | Autochthonous Lacticaseibacillus rhamnosus strains as probiotic candidates: comprehensive in vitro and in silico characterization |
| title_short | Autochthonous Lacticaseibacillus rhamnosus strains as probiotic candidates: comprehensive in vitro and in silico characterization |
| title_sort | autochthonous lacticaseibacillus rhamnosus strains as probiotic candidates comprehensive in vitro and in silico characterization |
| topic | Lactic Acid Bacteria Probiotics Genomics Milk Products Bacteria Acidoláctica Probióticos Genómica Experimentación in Vitro Productos Lácteos Lacticaseibacillus rhamnosus |
| url | http://hdl.handle.net/20.500.12123/21132 https://www.sciencedirect.com/science/article/abs/pii/S2212429225022448 https://doi.org/10.1016/j.fbio.2025.108066 |
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