Employment of pqqE gene as molecular marker for the traceability of Gram negative phosphate solubilizing bacteria associated to plants
Insoluble phosphorous compounds solubilization by soil bacteria is of great relevance since it puts available the phosphorus to be used by plants. The production of organic acids is the main microbiological mechanism by which insoluble inorganic phosphorus compounds are solubilized. In Gram negative...
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/21125 https://link.springer.com/article/10.1007/s00294-024-01296-4 https://doi.org/10.1007/s00294-024-01296-4 |
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| author | Anzuay, María Soledad Chiatti, Mario Hernán Intelangelo, Ariana Belén Ludueña, Liliana Mercedes Pin Viso, Natalia Daniela Angelini, Jorge Guillermo Taurian, Tania |
| author_browse | Angelini, Jorge Guillermo Anzuay, María Soledad Chiatti, Mario Hernán Intelangelo, Ariana Belén Ludueña, Liliana Mercedes Pin Viso, Natalia Daniela Taurian, Tania |
| author_facet | Anzuay, María Soledad Chiatti, Mario Hernán Intelangelo, Ariana Belén Ludueña, Liliana Mercedes Pin Viso, Natalia Daniela Angelini, Jorge Guillermo Taurian, Tania |
| author_sort | Anzuay, María Soledad |
| collection | INTA Digital |
| description | Insoluble phosphorous compounds solubilization by soil bacteria is of great relevance since it puts available the phosphorus to be used by plants. The production of organic acids is the main microbiological mechanism by which insoluble inorganic phosphorus compounds are solubilized. In Gram negative bacteria, gluconic acid is synthesized by the activity of the holoenzyme glucose dehydrogenase-pyrroloquinoline quinine named GDH-PQQ. The use of marker genes is a very useful tool to evaluate the persistence of the introduced bacteria and allow to follow-up the effect of biotic and abiotic factors on these beneficial microorganisms in the soil. In previous studies we detected the presence of the pqqE gene in a great percentage of both non-culturable and culturable native soil bacteria. The objective of this study was to analyze the phylogeny of the sequence of pqqE gene and its potential for the study of phosphate solubilizing bacteria from pure and mixed bacterial cultures and rhizospheric soil samples. For this, the presence of the pqqE gene in the genome of phosphate solubilizing bacteria that belong to several bacteria was determined by PCR. Also, this gene was analyzed from mixed bacterial cultures and rhizospheric soil associated to peanut plants inoculated or not with phosphate solubilizing bacteria. For this, degenerate primers designed from several bacterial genera and specific primers for the genus Pseudomonas spp., designed in this study, were used. DNA template used from simple or mixed bacterial cultures and from rhizospheric soil samples was obtained using two different DNA extraction techniques. Results indicated that pqqE gene amplification product was found in the genome of all Gram negative phosphate solubilizing bacteria analyzed. It was possible to detect this gene in the DNA obtained from mixed cultures where these bacteria grew in interaction with other microorganisms and in that obtained from rhizospheric soil samples inoculated or not with these bacteria. The phylogenetic analysis indicated that pqqE gene is a conserved gene within related genera. In conclusion, pqqE gene could be a potential marker for the study of phosphate solubilizing bacterial populations |
| format | Artículo |
| id | INTA21125 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA211252025-02-04T14:20:09Z Employment of pqqE gene as molecular marker for the traceability of Gram negative phosphate solubilizing bacteria associated to plants Anzuay, María Soledad Chiatti, Mario Hernán Intelangelo, Ariana Belén Ludueña, Liliana Mercedes Pin Viso, Natalia Daniela Angelini, Jorge Guillermo Taurian, Tania Arachis hypogaea Marcadores Genéticos Factor Abiótico Factor Biótico Filogenia Bacterias del Suelo Bacteria Gram Negativa Genetic Markers Abiotic Factors Biotic Factors Phylogeny Soil Bacteria Gram-negative Bacteria Pyrroloquinoline Quinone Biogenesis Phosphate Solubilizing Bacteria pqqE Gene Insoluble phosphorous compounds solubilization by soil bacteria is of great relevance since it puts available the phosphorus to be used by plants. The production of organic acids is the main microbiological mechanism by which insoluble inorganic phosphorus compounds are solubilized. In Gram negative bacteria, gluconic acid is synthesized by the activity of the holoenzyme glucose dehydrogenase-pyrroloquinoline quinine named GDH-PQQ. The use of marker genes is a very useful tool to evaluate the persistence of the introduced bacteria and allow to follow-up the effect of biotic and abiotic factors on these beneficial microorganisms in the soil. In previous studies we detected the presence of the pqqE gene in a great percentage of both non-culturable and culturable native soil bacteria. The objective of this study was to analyze the phylogeny of the sequence of pqqE gene and its potential for the study of phosphate solubilizing bacteria from pure and mixed bacterial cultures and rhizospheric soil samples. For this, the presence of the pqqE gene in the genome of phosphate solubilizing bacteria that belong to several bacteria was determined by PCR. Also, this gene was analyzed from mixed bacterial cultures and rhizospheric soil associated to peanut plants inoculated or not with phosphate solubilizing bacteria. For this, degenerate primers designed from several bacterial genera and specific primers for the genus Pseudomonas spp., designed in this study, were used. DNA template used from simple or mixed bacterial cultures and from rhizospheric soil samples was obtained using two different DNA extraction techniques. Results indicated that pqqE gene amplification product was found in the genome of all Gram negative phosphate solubilizing bacteria analyzed. It was possible to detect this gene in the DNA obtained from mixed cultures where these bacteria grew in interaction with other microorganisms and in that obtained from rhizospheric soil samples inoculated or not with these bacteria. The phylogenetic analysis indicated that pqqE gene is a conserved gene within related genera. In conclusion, pqqE gene could be a potential marker for the study of phosphate solubilizing bacterial populations Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Anzuay, María Soledad. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; Argentina Fil: Anzuay, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; Argentina Fil: Chiatti, Mario Hernán. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; Argentina Fil: Chiatti, Mario Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; Argentina Fil: Intelangelo, Ariana Belén. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; Argentina Fil: Intelangelo, Ariana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; Argentina Fil: Ludueña, Liliana Mercedes. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; Argentina Fil: Ludueña, Liliana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; Argentina Fil: Pin Viso, Natalia Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Pin Viso, Natalia Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Pin Viso, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Angelini, Jorge Guillermo. Universidad Nacional de Río Cuarto (UNRC). Instituto de Investigaciones Agrobiotecnológicas; Argentina Fil: Angelini, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; Argentina Fil: Taurian, Tania. Universidad Nacional de Río Cuarto (UNRC). Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Instituto de Investigaciones Agrobiotecnológicas; Argentina Fil: Taurian, Tania. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones Agrobiotecnológicas; Argentina 2025-02-04T14:10:29Z 2025-02-04T14:10:29Z 2024-08-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21125 https://link.springer.com/article/10.1007/s00294-024-01296-4 0172-8083 (impreso) 1432-0983 (online) https://doi.org/10.1007/s00294-024-01296-4 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Current Genetics 70 : article number: 12 (August 2024) |
| spellingShingle | Arachis hypogaea Marcadores Genéticos Factor Abiótico Factor Biótico Filogenia Bacterias del Suelo Bacteria Gram Negativa Genetic Markers Abiotic Factors Biotic Factors Phylogeny Soil Bacteria Gram-negative Bacteria Pyrroloquinoline Quinone Biogenesis Phosphate Solubilizing Bacteria pqqE Gene Anzuay, María Soledad Chiatti, Mario Hernán Intelangelo, Ariana Belén Ludueña, Liliana Mercedes Pin Viso, Natalia Daniela Angelini, Jorge Guillermo Taurian, Tania Employment of pqqE gene as molecular marker for the traceability of Gram negative phosphate solubilizing bacteria associated to plants |
| title | Employment of pqqE gene as molecular marker for the traceability of Gram negative phosphate solubilizing bacteria associated to plants |
| title_full | Employment of pqqE gene as molecular marker for the traceability of Gram negative phosphate solubilizing bacteria associated to plants |
| title_fullStr | Employment of pqqE gene as molecular marker for the traceability of Gram negative phosphate solubilizing bacteria associated to plants |
| title_full_unstemmed | Employment of pqqE gene as molecular marker for the traceability of Gram negative phosphate solubilizing bacteria associated to plants |
| title_short | Employment of pqqE gene as molecular marker for the traceability of Gram negative phosphate solubilizing bacteria associated to plants |
| title_sort | employment of pqqe gene as molecular marker for the traceability of gram negative phosphate solubilizing bacteria associated to plants |
| topic | Arachis hypogaea Marcadores Genéticos Factor Abiótico Factor Biótico Filogenia Bacterias del Suelo Bacteria Gram Negativa Genetic Markers Abiotic Factors Biotic Factors Phylogeny Soil Bacteria Gram-negative Bacteria Pyrroloquinoline Quinone Biogenesis Phosphate Solubilizing Bacteria pqqE Gene |
| url | http://hdl.handle.net/20.500.12123/21125 https://link.springer.com/article/10.1007/s00294-024-01296-4 https://doi.org/10.1007/s00294-024-01296-4 |
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