Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste
Seeds of four native species of trees and shrubs (Larrea cuneifolia, Bulnesia retama, Plectrocarpa tetracantha and Prosopis flexuosa) were exposed to soil contaminated with As, Cu, Cd, and Zn from an abandoned gold mine to identify adaptation strategies. Several physiological, morpho-anatomical, and...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/21124 https://www.sciencedirect.com/science/article/pii/S0147651325000867?via%3Dihub https://doi.org/10.1016/j.ecoenv.2025.117750 |
| _version_ | 1855038341518458880 |
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| author | Heredia, Belén Diaz Recio, Pablo Young, Brian Jonathan Pacheco, Pablo Barcelo Zungre, Franco Hruby, Silvina Anabela Acevedo, Maria Andreína Mercado, María Inés Roqueiro, Gonzalo |
| author_browse | Acevedo, Maria Andreína Barcelo Zungre, Franco Diaz Recio, Pablo Heredia, Belén Hruby, Silvina Anabela Mercado, María Inés Pacheco, Pablo Roqueiro, Gonzalo Young, Brian Jonathan |
| author_facet | Heredia, Belén Diaz Recio, Pablo Young, Brian Jonathan Pacheco, Pablo Barcelo Zungre, Franco Hruby, Silvina Anabela Acevedo, Maria Andreína Mercado, María Inés Roqueiro, Gonzalo |
| author_sort | Heredia, Belén |
| collection | INTA Digital |
| description | Seeds of four native species of trees and shrubs (Larrea cuneifolia, Bulnesia retama, Plectrocarpa tetracantha and Prosopis flexuosa) were exposed to soil contaminated with As, Cu, Cd, and Zn from an abandoned gold mine to identify adaptation strategies. Several physiological, morpho-anatomical, and biochemical parameters were determined. The seed germination of L. cuneifolia, B. retama, and P. tetracantha was fully inhibited in 100 % contaminated soil. Toxicological endpoints as NOEC, LOEC and IC50 ranged from 10 % to 25 % of soil contaminated with mining waste. Radicle elongation was the most sensitive variable to high metal(loid) con centrations, except for L. cuneifolia that hypocotyl elongation was the most affected parameter. P. flexuosa was selected to evaluate biochemical biomarkers and morpho-anatomical parameters. It showed an increase in radicle diameter and central radicle cylinder. A concentration-dependent increase in the O2 ⋅- production was observed in radicle and cotyledon. A peak of the enzymatic activity of guaiacol peroxidase, ascorbate peroxidase and catalase enzymes in P. flexuosa seedlings showed a negative relationship between metal(loid) concentration and exposure time. After a drop in the enzymatic activity, an increase in the malondialdehyde content (lipid peroxidation) was observed. The tested native species could be useful for phytoremediation of soils with a very high degree of metal contamination. A further investigation should focus on strategies to improve soil physi cochemical characteristics for plant survival at highest contamination levels |
| format | info:ar-repo/semantics/artículo |
| id | INTA21124 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA211242025-02-04T13:44:44Z Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste Heredia, Belén Diaz Recio, Pablo Young, Brian Jonathan Pacheco, Pablo Barcelo Zungre, Franco Hruby, Silvina Anabela Acevedo, Maria Andreína Mercado, María Inés Roqueiro, Gonzalo Estrés Oxidativo Fitodecontaminación Polución del suelo Fitotoxicidad Plántula Organismos Nativos Oxidative Stress Phytoremediation Soil Pollution Phytotoxicity Seedlings Native Organisms Seeds of four native species of trees and shrubs (Larrea cuneifolia, Bulnesia retama, Plectrocarpa tetracantha and Prosopis flexuosa) were exposed to soil contaminated with As, Cu, Cd, and Zn from an abandoned gold mine to identify adaptation strategies. Several physiological, morpho-anatomical, and biochemical parameters were determined. The seed germination of L. cuneifolia, B. retama, and P. tetracantha was fully inhibited in 100 % contaminated soil. Toxicological endpoints as NOEC, LOEC and IC50 ranged from 10 % to 25 % of soil contaminated with mining waste. Radicle elongation was the most sensitive variable to high metal(loid) con centrations, except for L. cuneifolia that hypocotyl elongation was the most affected parameter. P. flexuosa was selected to evaluate biochemical biomarkers and morpho-anatomical parameters. It showed an increase in radicle diameter and central radicle cylinder. A concentration-dependent increase in the O2 ⋅- production was observed in radicle and cotyledon. A peak of the enzymatic activity of guaiacol peroxidase, ascorbate peroxidase and catalase enzymes in P. flexuosa seedlings showed a negative relationship between metal(loid) concentration and exposure time. After a drop in the enzymatic activity, an increase in the malondialdehyde content (lipid peroxidation) was observed. The tested native species could be useful for phytoremediation of soils with a very high degree of metal contamination. A further investigation should focus on strategies to improve soil physi cochemical characteristics for plant survival at highest contamination levels EEA San Juan Fil: Heredia, Belén. Universidad Nacional de San Juan (UNSJ). Facultad de Ingeniería; Argentina Fil: Heredia, Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina Fil: Diaz Recio, Pablo. Universidad Nacional de San Juan (UNSJ). Facultad de Ingeniería; Argentina Fil: Young, Brian Jonathan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Pacheco, Pablo. Universidad Nacional de San Luis (UNSL). Instituto de Química San Luis; Argentina Fil: Pacheco, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Química San Luis; Argentina Fil: Barcelo Zungre, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina Fil: Barcelo Zungre, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico San Juan; Argentina Fil: Hruby, Silvina Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina Fil: Hruby, Silvina Anabela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro Científico Tecnológico San Juan; Argentina Fil: Acevedo, Maria Andreína. Fundación Miguel Lillo. Instituto de Morfología Vegetal; Argentina Fil: Mercado, María Inés. Fundación Miguel Lillo. Instituto de Morfología Vegetal; Argentina Fil: Roqueiro, Gonzalo. Universidad Nacional de San Juan (UNSJ). Facultad de Ingeniería; Argentina Fil: Roqueiro, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina 2025-02-04T13:30:13Z 2025-02-04T13:30:13Z 2025-01-22 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21124 https://www.sciencedirect.com/science/article/pii/S0147651325000867?via%3Dihub 0147-6513 https://doi.org/10.1016/j.ecoenv.2025.117750 eng info:eu-repograntAgreement/INTA/2023-PD-L03-I096, Estrategias de restauración, valoración y conservación de la biodiversidad y servicios ecosistemicos info:eu-repograntAgreement/INTA/2023-PE-L02-I003, Gestión sostenible de los recursos naturales para la producción y la población rural en ambientes áridos, con énfasis en el recurso hídrico info:eu-repograntAgreement/INTA/2023-PD-L04-I122, Gestión de las biomasas del SAB y estrategias tecnológicas para su transformación en bioproductos de valor agregado info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Ecotoxicology and Environmental Safety 290 (1) : 117750 (Enero 2025) |
| spellingShingle | Estrés Oxidativo Fitodecontaminación Polución del suelo Fitotoxicidad Plántula Organismos Nativos Oxidative Stress Phytoremediation Soil Pollution Phytotoxicity Seedlings Native Organisms Heredia, Belén Diaz Recio, Pablo Young, Brian Jonathan Pacheco, Pablo Barcelo Zungre, Franco Hruby, Silvina Anabela Acevedo, Maria Andreína Mercado, María Inés Roqueiro, Gonzalo Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste |
| title | Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste |
| title_full | Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste |
| title_fullStr | Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste |
| title_full_unstemmed | Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste |
| title_short | Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste |
| title_sort | strategies of physiological morpho anatomical and biochemical adaptation in seedlings of native species exposed to mining waste |
| topic | Estrés Oxidativo Fitodecontaminación Polución del suelo Fitotoxicidad Plántula Organismos Nativos Oxidative Stress Phytoremediation Soil Pollution Phytotoxicity Seedlings Native Organisms |
| url | http://hdl.handle.net/20.500.12123/21124 https://www.sciencedirect.com/science/article/pii/S0147651325000867?via%3Dihub https://doi.org/10.1016/j.ecoenv.2025.117750 |
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