Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia
BACKGROUND: Azinphos-methyl is the main insecticide used to control codling moth on apple and pears in Northern Patagonia. The aim of this study was to evaluate the toxicological and biochemical response of diapausing larvae of codling moth in orchards subjected to different insecticide selection pr...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Español |
| Publicado: |
Wiley
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/21113 https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.1582 https://doi.org/10.1002/ps.1582 |
| _version_ | 1855038348688621568 |
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| author | Soleño, Jimena Anguiano, Olga Liliana Pechen de D’Angelo, Ana Cichon, Liliana Fernandez, Dario Eduardo Montagna, Cristina Mónica |
| author_browse | Anguiano, Olga Liliana Cichon, Liliana Fernandez, Dario Eduardo Montagna, Cristina Mónica Pechen de D’Angelo, Ana Soleño, Jimena |
| author_facet | Soleño, Jimena Anguiano, Olga Liliana Pechen de D’Angelo, Ana Cichon, Liliana Fernandez, Dario Eduardo Montagna, Cristina Mónica |
| author_sort | Soleño, Jimena |
| collection | INTA Digital |
| description | BACKGROUND: Azinphos-methyl is the main insecticide used to control codling moth on apple and pears in Northern Patagonia. The aim of this study was to evaluate the toxicological and biochemical response of diapausing larvae of codling moth in orchards subjected to different insecticide selection pressure.
RESULTS: Dose–mortality assays with azinphos-methyl in diapausing larvae of Cydia pomonella L. showed significant differences between the LD95 from a population collected in one untreated orchard (2.52 μg moth−1) compared with that in a laboratory-susceptible population (0.33 μg moth−1). Toxicity to azinphos-methyl in field populations of diapausing larvae collected during 2003–2005 was evaluated by topical application of a discriminating dose (2.5 μg moth−1) that was obtained from larvae collected in the untreated orchard (field reference strain). Significantly lower mortality (37.71–84.21%) was observed in three out of eight field populations compared with that in the field reference strain.Most of the field populations showed higher esterase activity than that determined in both the laboratory susceptible and the field reference strains. Moreover, there was a high association between
esterase activity and mortality (R2 = 0.64) among the field populations. On the other hand, a poor correlation was observed between glutathione S-transferase activity and mortality (R2 = 0.33) among larvae collected from different orchards.
CONCLUSIONS: All the field populations evaluated exhibited some degree of azinphos-methyl tolerance in relation to the laboratory susceptible strain. Biochemical results demonstrated that esterases are at least one of the principal mechanisms involved in tolerance to this insecticide.
© 2008 Society of Chemical Industry |
| format | info:ar-repo/semantics/artículo |
| id | INTA21113 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Español |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA211132025-02-13T15:11:28Z Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia Soleño, Jimena Anguiano, Olga Liliana Pechen de D’Angelo, Ana Cichon, Liliana Fernandez, Dario Eduardo Montagna, Cristina Mónica Cydia Pomonella Resistencia a las plagas Control de plagas Insecticides Insecticidas Esterasas Rio Negro (Argentina) Neuquén Pest resistance Pest control Esterases Codling Moth Polilla de la Manzana Carpocapsa Azinphos-Methyl Azinfos-Metil Glutathione S-Transferases Glutatión S-Transferasas BACKGROUND: Azinphos-methyl is the main insecticide used to control codling moth on apple and pears in Northern Patagonia. The aim of this study was to evaluate the toxicological and biochemical response of diapausing larvae of codling moth in orchards subjected to different insecticide selection pressure. RESULTS: Dose–mortality assays with azinphos-methyl in diapausing larvae of Cydia pomonella L. showed significant differences between the LD95 from a population collected in one untreated orchard (2.52 μg moth−1) compared with that in a laboratory-susceptible population (0.33 μg moth−1). Toxicity to azinphos-methyl in field populations of diapausing larvae collected during 2003–2005 was evaluated by topical application of a discriminating dose (2.5 μg moth−1) that was obtained from larvae collected in the untreated orchard (field reference strain). Significantly lower mortality (37.71–84.21%) was observed in three out of eight field populations compared with that in the field reference strain.Most of the field populations showed higher esterase activity than that determined in both the laboratory susceptible and the field reference strains. Moreover, there was a high association between esterase activity and mortality (R2 = 0.64) among the field populations. On the other hand, a poor correlation was observed between glutathione S-transferase activity and mortality (R2 = 0.33) among larvae collected from different orchards. CONCLUSIONS: All the field populations evaluated exhibited some degree of azinphos-methyl tolerance in relation to the laboratory susceptible strain. Biochemical results demonstrated that esterases are at least one of the principal mechanisms involved in tolerance to this insecticide. © 2008 Society of Chemical Industry EEA Alto Valle Fil: Soleño, Jimena. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; Argentina Fil: Anguiano, Olga Liliana. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; Argentina Fil: Pechen de D’Angelo, Ana. Universidad Nacional del Comahue (UNCo). Facultad de Ingeniería (FaIn). Departamento de Química; Argentina Fil: Cichón, Liliana Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina Fil: Fernánez, Darío Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina Fil: Montagna, Cristina Mónica. Universidad Nacional del Comahue (UNCo). Escuela Superior de Salud y Ambiente; Argentina 2025-01-31T14:28:48Z 2025-01-31T14:28:48Z 2008-03-31 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21113 https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.1582 1526-4998 1526-498X https://doi.org/10.1002/ps.1582 spa info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Pest Management Science 64 (9): 964–970 (September 2008) |
| spellingShingle | Cydia Pomonella Resistencia a las plagas Control de plagas Insecticides Insecticidas Esterasas Rio Negro (Argentina) Neuquén Pest resistance Pest control Esterases Codling Moth Polilla de la Manzana Carpocapsa Azinphos-Methyl Azinfos-Metil Glutathione S-Transferases Glutatión S-Transferasas Soleño, Jimena Anguiano, Olga Liliana Pechen de D’Angelo, Ana Cichon, Liliana Fernandez, Dario Eduardo Montagna, Cristina Mónica Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia |
| title | Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia |
| title_full | Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia |
| title_fullStr | Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia |
| title_full_unstemmed | Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia |
| title_short | Toxicological and biochemical response to azinphos-methyl in Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia |
| title_sort | toxicological and biochemical response to azinphos methyl in cydia pomonella l lepidoptera tortricidae among orchards from the argentinian patagonia |
| topic | Cydia Pomonella Resistencia a las plagas Control de plagas Insecticides Insecticidas Esterasas Rio Negro (Argentina) Neuquén Pest resistance Pest control Esterases Codling Moth Polilla de la Manzana Carpocapsa Azinphos-Methyl Azinfos-Metil Glutathione S-Transferases Glutatión S-Transferasas |
| url | http://hdl.handle.net/20.500.12123/21113 https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.1582 https://doi.org/10.1002/ps.1582 |
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