Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation
Azospirillum argentinense Az19 is an osmotolerant plant growth-promoting bacterium that protects maize plants from drought. In this work, we explored the role of trehalose in the superior performance of Az19 under stress. The trehalase-coding gene treF was constitutively expressed in Az19 through a...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/21088 https://link.springer.com/article/10.1007/s00253-024-13391-0 https://doi.org/10.1007/s00253-024-13391-0 |
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| author | Garcia, Julia Elena Pagnussat, Luciana Anabella Amenta, Melina B. Casanovas, E. Mabel Diaz, Pablo R. Labarthe, María Mercedes Martino, María V. Groppa, María Daniela Creus, Cecilia Mónica Maroniche, Guillermo Andrés |
| author_browse | Amenta, Melina B. Casanovas, E. Mabel Creus, Cecilia Mónica Diaz, Pablo R. Garcia, Julia Elena Groppa, María Daniela Labarthe, María Mercedes Maroniche, Guillermo Andrés Martino, María V. Pagnussat, Luciana Anabella |
| author_facet | Garcia, Julia Elena Pagnussat, Luciana Anabella Amenta, Melina B. Casanovas, E. Mabel Diaz, Pablo R. Labarthe, María Mercedes Martino, María V. Groppa, María Daniela Creus, Cecilia Mónica Maroniche, Guillermo Andrés |
| author_sort | Garcia, Julia Elena |
| collection | INTA Digital |
| description | Azospirillum argentinense Az19 is an osmotolerant plant growth-promoting bacterium that protects maize plants from drought. In this work, we explored the role of trehalose in the superior performance of Az19 under stress. The trehalase-coding gene treF was constitutively expressed in Az19 through a miniTn7 system. The resulting recombinant strain, Az19F, did not accumulate trehalose, was affected in its capacity to cope with salt-, osmotic-, and UV-stress, and showed higher reactive oxygen species levels. Physiological alterations were also observed under normal conditions, such as increased growth in biofilms, higher motility, and decreased auxin secretion. Even so, the capacity of Az19F to colonize maize roots was not affected, either under normal or drought conditions. When inoculated in maize, both Az19 and Az19F strains promoted plant growth similarly under normal irrigation. However, unlike Az19, the trehalose-deficient strain Az19F could not improve the height, aerial fresh weight, or relative water content of maize plants under drought. Notably, Az19F triggered an exacerbated oxidative response in the plants, resulting in higher levels of antioxidant and phenolic compounds. We conclude that the role of trehalose metabolism in A. argentinense Az19 transcends stress tolerance, being also important for normal bacterial physiology and its plant growth-promoting activity under drought. |
| format | Artículo |
| id | INTA21088 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA210882025-01-27T15:41:03Z Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation Garcia, Julia Elena Pagnussat, Luciana Anabella Amenta, Melina B. Casanovas, E. Mabel Diaz, Pablo R. Labarthe, María Mercedes Martino, María V. Groppa, María Daniela Creus, Cecilia Mónica Maroniche, Guillermo Andrés Estrés Abiótico Biofertilizantes Maíz Azospirillum Trehalosa Zea mays Abiotic Stress Biofertilizers Maize Trehalose Azospirillum argentinense Az19 Inoculante Inoculant Azospirillum argentinense Az19 is an osmotolerant plant growth-promoting bacterium that protects maize plants from drought. In this work, we explored the role of trehalose in the superior performance of Az19 under stress. The trehalase-coding gene treF was constitutively expressed in Az19 through a miniTn7 system. The resulting recombinant strain, Az19F, did not accumulate trehalose, was affected in its capacity to cope with salt-, osmotic-, and UV-stress, and showed higher reactive oxygen species levels. Physiological alterations were also observed under normal conditions, such as increased growth in biofilms, higher motility, and decreased auxin secretion. Even so, the capacity of Az19F to colonize maize roots was not affected, either under normal or drought conditions. When inoculated in maize, both Az19 and Az19F strains promoted plant growth similarly under normal irrigation. However, unlike Az19, the trehalose-deficient strain Az19F could not improve the height, aerial fresh weight, or relative water content of maize plants under drought. Notably, Az19F triggered an exacerbated oxidative response in the plants, resulting in higher levels of antioxidant and phenolic compounds. We conclude that the role of trehalose metabolism in A. argentinense Az19 transcends stress tolerance, being also important for normal bacterial physiology and its plant growth-promoting activity under drought. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Pagnussat, Luciana Anabella. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC); Argentina Fil: Amenta, Melina B. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Casanovas, E. Mabel. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Diaz, Pablo R. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Diaz, Pablo R. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Labarthe, María Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Martino, María V. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Groppa, María Daniela. Universidad de Buenos Aires (UBA). Instituto de Química y Fisicoquímica Biológicas (IQUIFIB); Argentina Fil: Groppa, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Química y Fisicoquímica Biológicas (IQUIFIB); Argentina Fil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina 2025-01-27T14:50:28Z 2025-01-27T14:50:28Z 2024-12-27 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21088 https://link.springer.com/article/10.1007/s00253-024-13391-0 Applied Microbiology and Biotechnology 108 : 543 (Diciembre 2024) 1432-0614 (Online) 0175-7598 https://doi.org/10.1007/s00253-024-13391-0 eng info:eu-repograntAgreement/INTA/2023-PE-L04-I073, Desarrollo de bioinsumos y su integración en estrategias de manejo de adversidades bióticas y abióticas en cultivos agrícolas y forestales info:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejo info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Applied Microbiology and Biotechnology 108 : 543 (Diciembre 2024) |
| spellingShingle | Estrés Abiótico Biofertilizantes Maíz Azospirillum Trehalosa Zea mays Abiotic Stress Biofertilizers Maize Trehalose Azospirillum argentinense Az19 Inoculante Inoculant Garcia, Julia Elena Pagnussat, Luciana Anabella Amenta, Melina B. Casanovas, E. Mabel Diaz, Pablo R. Labarthe, María Mercedes Martino, María V. Groppa, María Daniela Creus, Cecilia Mónica Maroniche, Guillermo Andrés Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation |
| title | Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation |
| title_full | Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation |
| title_fullStr | Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation |
| title_full_unstemmed | Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation |
| title_short | Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation |
| title_sort | maize drought protection by azospirillum argentinense az19 requires bacterial trehalose accumulation |
| topic | Estrés Abiótico Biofertilizantes Maíz Azospirillum Trehalosa Zea mays Abiotic Stress Biofertilizers Maize Trehalose Azospirillum argentinense Az19 Inoculante Inoculant |
| url | http://hdl.handle.net/20.500.12123/21088 https://link.springer.com/article/10.1007/s00253-024-13391-0 https://doi.org/10.1007/s00253-024-13391-0 |
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