Comparison of two serological diagnosis tests for bovine paratuberculosis
Mycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of paratuberculosis (PTBC), a chronic infectious granulomatous enteritis of ruminants. The PTBC diagnosis with commercial ELISA has limitations in sensitivity and specificity, and its results depend on the state of progress of the...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2025
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/21053 https://link.springer.com/article/10.1007/s11259-024-10363-7 https://doi.org/10.1007/s11259-024-10363-7 |
| _version_ | 1855486683444674560 |
|---|---|
| author | Tieri, Silvina Morsella, Claudia Graciela Mendez, Laura Luján Vasini, B. Garro, Carlos Javier Paolicchi, Fernando |
| author_browse | Garro, Carlos Javier Mendez, Laura Luján Morsella, Claudia Graciela Paolicchi, Fernando Tieri, Silvina Vasini, B. |
| author_facet | Tieri, Silvina Morsella, Claudia Graciela Mendez, Laura Luján Vasini, B. Garro, Carlos Javier Paolicchi, Fernando |
| author_sort | Tieri, Silvina |
| collection | INTA Digital |
| description | Mycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of paratuberculosis (PTBC), a chronic infectious granulomatous enteritis of ruminants. The PTBC diagnosis with commercial ELISA has limitations in sensitivity and specificity, and its results depend on the state of progress of the disease. This research aimed to evaluate two different ELISAs: (a) an “in-house” ELISA with a sonicated antigen obtained from a MAP I47 strain, and (b) a commercial ELISA. In total, the evaluated sample consisted of 394 bovine serum samples from 12 farms in Argentina with high (5–9%) and low (≤ 0.05%) prevalence of PTBC. The evaluation of the new antigen (2.5 µg/mL) was against a 1:50 dilution of the M. phlei faced sera. The cut-off point, sensitivity, and specificity determinations of both techniques were by ROC curve analysis. The area under the curve for the I47 ELISA was 0.9 (CI 95%, 0.93–0.97). With a cut-off point of 8.8%, the sensitivity was 84.3% and the specificity 96.6%. The agreement between both techniques was 0.7 (CI 95%, 0.6–0.8). These results indicate a high discriminative capacity to differentiate positive and negative bovine sera of MAP infection with the I47 ELISA. This result would represent an advantage to dispense with the imported kit. |
| format | Artículo |
| id | INTA21053 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA210532025-02-13T15:05:03Z Comparison of two serological diagnosis tests for bovine paratuberculosis Tieri, Silvina Morsella, Claudia Graciela Mendez, Laura Luján Vasini, B. Garro, Carlos Javier Paolicchi, Fernando Ganado Bovino Enfermedades de los Animales Inmunodiagnóstico Cattle Animal Diseases Paratuberculosis Mycobacterium avium subsp. paratuberculosis Immunodiagnosis Mycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of paratuberculosis (PTBC), a chronic infectious granulomatous enteritis of ruminants. The PTBC diagnosis with commercial ELISA has limitations in sensitivity and specificity, and its results depend on the state of progress of the disease. This research aimed to evaluate two different ELISAs: (a) an “in-house” ELISA with a sonicated antigen obtained from a MAP I47 strain, and (b) a commercial ELISA. In total, the evaluated sample consisted of 394 bovine serum samples from 12 farms in Argentina with high (5–9%) and low (≤ 0.05%) prevalence of PTBC. The evaluation of the new antigen (2.5 µg/mL) was against a 1:50 dilution of the M. phlei faced sera. The cut-off point, sensitivity, and specificity determinations of both techniques were by ROC curve analysis. The area under the curve for the I47 ELISA was 0.9 (CI 95%, 0.93–0.97). With a cut-off point of 8.8%, the sensitivity was 84.3% and the specificity 96.6%. The agreement between both techniques was 0.7 (CI 95%, 0.6–0.8). These results indicate a high discriminative capacity to differentiate positive and negative bovine sera of MAP infection with the I47 ELISA. This result would represent an advantage to dispense with the imported kit. EEA Balcarce Fil: Tieri, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Balcarce; Argentina Fil: Tieri, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Mendez, Laura Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Vasini, B. University of North Texas Health Science Center; Estados Unidos Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Paolicchi, Fernando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. 2025-01-22T16:08:28Z 2025-01-22T16:08:28Z 2024-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21053 https://link.springer.com/article/10.1007/s11259-024-10363-7 0165-7380 (print) 1573-7446 (online) https://doi.org/10.1007/s11259-024-10363-7 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Veterinary Research Communications 48 : 2645-2650, (2024) |
| spellingShingle | Ganado Bovino Enfermedades de los Animales Inmunodiagnóstico Cattle Animal Diseases Paratuberculosis Mycobacterium avium subsp. paratuberculosis Immunodiagnosis Tieri, Silvina Morsella, Claudia Graciela Mendez, Laura Luján Vasini, B. Garro, Carlos Javier Paolicchi, Fernando Comparison of two serological diagnosis tests for bovine paratuberculosis |
| title | Comparison of two serological diagnosis tests for bovine paratuberculosis |
| title_full | Comparison of two serological diagnosis tests for bovine paratuberculosis |
| title_fullStr | Comparison of two serological diagnosis tests for bovine paratuberculosis |
| title_full_unstemmed | Comparison of two serological diagnosis tests for bovine paratuberculosis |
| title_short | Comparison of two serological diagnosis tests for bovine paratuberculosis |
| title_sort | comparison of two serological diagnosis tests for bovine paratuberculosis |
| topic | Ganado Bovino Enfermedades de los Animales Inmunodiagnóstico Cattle Animal Diseases Paratuberculosis Mycobacterium avium subsp. paratuberculosis Immunodiagnosis |
| url | http://hdl.handle.net/20.500.12123/21053 https://link.springer.com/article/10.1007/s11259-024-10363-7 https://doi.org/10.1007/s11259-024-10363-7 |
| work_keys_str_mv | AT tierisilvina comparisonoftwoserologicaldiagnosistestsforbovineparatuberculosis AT morsellaclaudiagraciela comparisonoftwoserologicaldiagnosistestsforbovineparatuberculosis AT mendezlauralujan comparisonoftwoserologicaldiagnosistestsforbovineparatuberculosis AT vasinib comparisonoftwoserologicaldiagnosistestsforbovineparatuberculosis AT garrocarlosjavier comparisonoftwoserologicaldiagnosistestsforbovineparatuberculosis AT paolicchifernando comparisonoftwoserologicaldiagnosistestsforbovineparatuberculosis |