Exploring sugar allocation and metabolic shifts in cassava plants infected with Cassava common mosaic virus (CsCMV) under long-day photoperiod: diel changes in source and sink leaves
Cassava common mosaic virus (CsCMV) is a potexvirus that impairs chloroplast and metabolism, causing significant yield losses to cassava crops. Crop yield depends on diel rhythms, influencing carbon allocation and growth, and sugar signaling also impacting light–dark rhythms. This study aimed to e...
| Autores principales: | , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/21051 https://link.springer.com/article/10.1007/s10265-024-01595-4 https://doi.org/10.1007/s10265-024-01595-4 |
| _version_ | 1855486683242299392 |
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| author | Zanini, Andrea Alejandra Dominguez Muñoz, Martin Ceferino Rodriguez, Marianela |
| author_browse | Dominguez Muñoz, Martin Ceferino Rodriguez, Marianela Zanini, Andrea Alejandra |
| author_facet | Zanini, Andrea Alejandra Dominguez Muñoz, Martin Ceferino Rodriguez, Marianela |
| author_sort | Zanini, Andrea Alejandra |
| collection | INTA Digital |
| description | Cassava common mosaic virus (CsCMV) is a potexvirus that impairs chloroplast and metabolism, causing significant yield
losses to cassava crops. Crop yield depends on diel rhythms, influencing carbon allocation and growth, and sugar signaling
also impacting light–dark rhythms. This study aimed to elucidate the early impact of CsCMV infection on diel carbon allocation,
metabolism, and defense mechanisms in both source and sink cassava leaves before storage root bulking. Soluble sugar
and starch concentrations were examined over a 24-h cycle (16:8 photoperiod) in CsCMV-infected plants. The expression
of an array of genes—carbohydrate metabolism, SnRK1 activity marker, defense, circadian marker—was analyzed at ZT6,
ZT16 and ZT24/ZT0. In CsCMV-infected source leaves, at ZT6, sucrose increased whereas glucose, fructose and sucrose
rose at night. An increase in Suc:hexose ratio and upregulation of SnRK1 activity marker genes and PR1 transcripts were
found in infected leaves, suggesting a combination of altered carbon metabolism and defense response mechanisms against
the viral infection. GIGANTEA, a clock-controlled gene, showed a reduced expression in infected leaves at ZT6 and ZT24/
ZT0, suggesting a circadian phase shift compared with uninfected control plants. Additionally, starch mobilization transcripts
were downregulated at ZT24/ZT0, though starch content remained unchanged during the 24-h cycle. In sink leaves,
a transient peak of maltose (ZT6) was observed. Our findings suggest that CsCMV disrupts the plant's natural rhythms of
sugar metabolism and allocation. Spikes in sucrose levels may serve as infection signals in the internal daily clock of the
plant, influencing plant responses during the cassava-CsCMV interaction. |
| format | Artículo |
| id | INTA21051 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA210512025-01-22T15:07:39Z Exploring sugar allocation and metabolic shifts in cassava plants infected with Cassava common mosaic virus (CsCMV) under long-day photoperiod: diel changes in source and sink leaves Zanini, Andrea Alejandra Dominguez Muñoz, Martin Ceferino Rodriguez, Marianela Mandioca Relaciones Fuente Sumidero Fotoperiodismo Cassava Source-sink Relations Photoperiodicity CsCMV Plant-virus interaction Sugar Allocation Sugar Signal Cassava common mosaic virus (CsCMV) is a potexvirus that impairs chloroplast and metabolism, causing significant yield losses to cassava crops. Crop yield depends on diel rhythms, influencing carbon allocation and growth, and sugar signaling also impacting light–dark rhythms. This study aimed to elucidate the early impact of CsCMV infection on diel carbon allocation, metabolism, and defense mechanisms in both source and sink cassava leaves before storage root bulking. Soluble sugar and starch concentrations were examined over a 24-h cycle (16:8 photoperiod) in CsCMV-infected plants. The expression of an array of genes—carbohydrate metabolism, SnRK1 activity marker, defense, circadian marker—was analyzed at ZT6, ZT16 and ZT24/ZT0. In CsCMV-infected source leaves, at ZT6, sucrose increased whereas glucose, fructose and sucrose rose at night. An increase in Suc:hexose ratio and upregulation of SnRK1 activity marker genes and PR1 transcripts were found in infected leaves, suggesting a combination of altered carbon metabolism and defense response mechanisms against the viral infection. GIGANTEA, a clock-controlled gene, showed a reduced expression in infected leaves at ZT6 and ZT24/ ZT0, suggesting a circadian phase shift compared with uninfected control plants. Additionally, starch mobilization transcripts were downregulated at ZT24/ZT0, though starch content remained unchanged during the 24-h cycle. In sink leaves, a transient peak of maltose (ZT6) was observed. Our findings suggest that CsCMV disrupts the plant's natural rhythms of sugar metabolism and allocation. Spikes in sucrose levels may serve as infection signals in the internal daily clock of the plant, influencing plant responses during the cassava-CsCMV interaction. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Zanini, Andrea Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Estudios Agropecuarios; Argentina Fil: Zanini, Andrea Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Unidad de Estudios Agropecuarios; Argentina Fil: Zanini, Andrea Alejandra. Donald Danforth Plant Science Center; Estados Unidos Fil: Dominguez Muñoz, Martin Ceferino. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; Argentina Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Unidad de Estudios Agropecuarios; Argentina Fil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Estudios Agropecuarios; Argentina Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina 2025-01-22T14:33:40Z 2025-01-22T14:33:40Z 2025-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21051 https://link.springer.com/article/10.1007/s10265-024-01595-4 0918-9440 1618-0860 (online) https://doi.org/10.1007/s10265-024-01595-4 eng info:eu-repograntAgreement/INTA/2019-PD-E4-I085-001/2019-PD-E4-I085-001/AR./Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógeno. info:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejo info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Journal of Plant Research 138 : 131–145 (2025) |
| spellingShingle | Mandioca Relaciones Fuente Sumidero Fotoperiodismo Cassava Source-sink Relations Photoperiodicity CsCMV Plant-virus interaction Sugar Allocation Sugar Signal Zanini, Andrea Alejandra Dominguez Muñoz, Martin Ceferino Rodriguez, Marianela Exploring sugar allocation and metabolic shifts in cassava plants infected with Cassava common mosaic virus (CsCMV) under long-day photoperiod: diel changes in source and sink leaves |
| title | Exploring sugar allocation and metabolic shifts in cassava plants infected with Cassava common mosaic virus (CsCMV) under long-day photoperiod: diel changes in source and sink leaves |
| title_full | Exploring sugar allocation and metabolic shifts in cassava plants infected with Cassava common mosaic virus (CsCMV) under long-day photoperiod: diel changes in source and sink leaves |
| title_fullStr | Exploring sugar allocation and metabolic shifts in cassava plants infected with Cassava common mosaic virus (CsCMV) under long-day photoperiod: diel changes in source and sink leaves |
| title_full_unstemmed | Exploring sugar allocation and metabolic shifts in cassava plants infected with Cassava common mosaic virus (CsCMV) under long-day photoperiod: diel changes in source and sink leaves |
| title_short | Exploring sugar allocation and metabolic shifts in cassava plants infected with Cassava common mosaic virus (CsCMV) under long-day photoperiod: diel changes in source and sink leaves |
| title_sort | exploring sugar allocation and metabolic shifts in cassava plants infected with cassava common mosaic virus cscmv under long day photoperiod diel changes in source and sink leaves |
| topic | Mandioca Relaciones Fuente Sumidero Fotoperiodismo Cassava Source-sink Relations Photoperiodicity CsCMV Plant-virus interaction Sugar Allocation Sugar Signal |
| url | http://hdl.handle.net/20.500.12123/21051 https://link.springer.com/article/10.1007/s10265-024-01595-4 https://doi.org/10.1007/s10265-024-01595-4 |
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