SPR Biosensing MUA/Poly-L-lysine platform for the detection of 2,4-Dinitrophenol as small molecule model system
Surface Plasmon Resonance assays are being developed as alternative biodetection methods for a great number of pesticides and toxins.These substances typically have low molecular weight, making it necessary to perform competitive inhibition immunoassays. In most of the cases, the strategy is to immo...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/2097 https://www.hindawi.com/journals/jnm/2016/5432656/ |
| _version_ | 1855482993561305088 |
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| author | Daza Millone, Maria Antonieta Ramirez, Eduardo A. Chain, Cecilia Yamil Crivaro, Andrea Romanin, David Emmanuel Rumbo, Martín Docena, Guillermo H. Cocco, Mauro D. Pedano, María Laura Fainstein, Alejandro Montoya, Jorgelina Ceferina Vela, Maria Elena Salvarezza, Roberto Carlos |
| author_browse | Chain, Cecilia Yamil Cocco, Mauro D. Crivaro, Andrea Daza Millone, Maria Antonieta Docena, Guillermo H. Fainstein, Alejandro Montoya, Jorgelina Ceferina Pedano, María Laura Ramirez, Eduardo A. Romanin, David Emmanuel Rumbo, Martín Salvarezza, Roberto Carlos Vela, Maria Elena |
| author_facet | Daza Millone, Maria Antonieta Ramirez, Eduardo A. Chain, Cecilia Yamil Crivaro, Andrea Romanin, David Emmanuel Rumbo, Martín Docena, Guillermo H. Cocco, Mauro D. Pedano, María Laura Fainstein, Alejandro Montoya, Jorgelina Ceferina Vela, Maria Elena Salvarezza, Roberto Carlos |
| author_sort | Daza Millone, Maria Antonieta |
| collection | INTA Digital |
| description | Surface Plasmon Resonance assays are being developed as alternative biodetection methods for a great number of pesticides and toxins.These substances typically have low molecular weight, making it necessary to perform competitive inhibition immunoassays. In most of the cases, the strategy is to immobilize a protein derivative of the analyte, which usually involves the appearance of nonspecific protein binding which limits the detection range of the assay. In this work we present results of a poly-L-lysine (Au-MUA-PLL) based sensor platform for quantitative determination of 2,4-dinitrophenol as model system for small molecular weight substances detection. The prepared sensor chip was characterized by means of Atomic Force Microscopy, Surface Plasmon Resonance, and Surface Enhanced Raman Spectroscopy. Experiments verified the absence of nonspecific protein adsorption to Au-MUA-PLL surfaces and the improvement of the competitive inhibition assays performance in comparison with single and mixed thiol self-assembled monolayers. The possibility of directly immobilizing 2,4-dinitrophenol to the poly-L-lysine containing platforms leads to an improvement in the detection of the soluble analyte by the competitive inhibition assay avoiding undesirable nonspecific protein adsorption.Therefore, Au-MUA-PLL surfaces constitute a suitable alternative for quantitative detection of small molecules when nonspecific adsorption cannot be avoided. |
| format | Artículo |
| id | INTA2097 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA20972018-03-26T15:57:19Z SPR Biosensing MUA/Poly-L-lysine platform for the detection of 2,4-Dinitrophenol as small molecule model system Daza Millone, Maria Antonieta Ramirez, Eduardo A. Chain, Cecilia Yamil Crivaro, Andrea Romanin, David Emmanuel Rumbo, Martín Docena, Guillermo H. Cocco, Mauro D. Pedano, María Laura Fainstein, Alejandro Montoya, Jorgelina Ceferina Vela, Maria Elena Salvarezza, Roberto Carlos 2,4-dinitrofenol Identificación Biosensores Biología Molecular Biosensors Identification Molecular Biology Superficies Au-MUA-PLL Surface Plasmon Resonance assays are being developed as alternative biodetection methods for a great number of pesticides and toxins.These substances typically have low molecular weight, making it necessary to perform competitive inhibition immunoassays. In most of the cases, the strategy is to immobilize a protein derivative of the analyte, which usually involves the appearance of nonspecific protein binding which limits the detection range of the assay. In this work we present results of a poly-L-lysine (Au-MUA-PLL) based sensor platform for quantitative determination of 2,4-dinitrophenol as model system for small molecular weight substances detection. The prepared sensor chip was characterized by means of Atomic Force Microscopy, Surface Plasmon Resonance, and Surface Enhanced Raman Spectroscopy. Experiments verified the absence of nonspecific protein adsorption to Au-MUA-PLL surfaces and the improvement of the competitive inhibition assays performance in comparison with single and mixed thiol self-assembled monolayers. The possibility of directly immobilizing 2,4-dinitrophenol to the poly-L-lysine containing platforms leads to an improvement in the detection of the soluble analyte by the competitive inhibition assay avoiding undesirable nonspecific protein adsorption.Therefore, Au-MUA-PLL surfaces constitute a suitable alternative for quantitative detection of small molecules when nonspecific adsorption cannot be avoided. Fil: Daza Millone, Maria Antonieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina Fil: Ramirez, Eduardo A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina Fil: Chain, Cecilia Yamil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina Fil: Crivaro, Andrea. Universidad Nacional de la Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Romanin, David Emmanuel. Universidad Nacional de la Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rumbo, Martín. Universidad Nacional de la Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Docena, Guillermo H. Universidad Nacional de la Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cocco, Mauro D. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche, Instituto Balseiro; Argentina Fil: Pedano, María Laura. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche, Instituto Balseiro; Argentina Fil: Fainstein, Alejandro. Comisión Nacional de Energía Atómica. Centro Atomico Bariloche, Instituto Balseiro; Argentina Fil: Montoya, Jorgelina Ceferina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina Fil: Vela, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina Fil: Salvarezza, Roberto Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina 2018-03-21T18:25:32Z 2018-03-21T18:25:32Z 2016-10-18 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2097 https://www.hindawi.com/journals/jnm/2016/5432656/ 1687-4110 1687-4129 (Online) eng info:eu-repograntAgreement/INTA/PNNAT/1128043/AR.Anguil, La Pampa/Los agroquímicos como fuente de contaminación difusa en agroecosistemas. info:eu-repograntAgreement/INTA/PNNAT/1128043/AR./Los agroquímicos como fuente de contaminación difusa en agroecosistemas. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Journal of nanomaterials : Article ID 5432656. (2016) |
| spellingShingle | 2,4-dinitrofenol Identificación Biosensores Biología Molecular Biosensors Identification Molecular Biology Superficies Au-MUA-PLL Daza Millone, Maria Antonieta Ramirez, Eduardo A. Chain, Cecilia Yamil Crivaro, Andrea Romanin, David Emmanuel Rumbo, Martín Docena, Guillermo H. Cocco, Mauro D. Pedano, María Laura Fainstein, Alejandro Montoya, Jorgelina Ceferina Vela, Maria Elena Salvarezza, Roberto Carlos SPR Biosensing MUA/Poly-L-lysine platform for the detection of 2,4-Dinitrophenol as small molecule model system |
| title | SPR Biosensing MUA/Poly-L-lysine platform for the detection of 2,4-Dinitrophenol as small molecule model system |
| title_full | SPR Biosensing MUA/Poly-L-lysine platform for the detection of 2,4-Dinitrophenol as small molecule model system |
| title_fullStr | SPR Biosensing MUA/Poly-L-lysine platform for the detection of 2,4-Dinitrophenol as small molecule model system |
| title_full_unstemmed | SPR Biosensing MUA/Poly-L-lysine platform for the detection of 2,4-Dinitrophenol as small molecule model system |
| title_short | SPR Biosensing MUA/Poly-L-lysine platform for the detection of 2,4-Dinitrophenol as small molecule model system |
| title_sort | spr biosensing mua poly l lysine platform for the detection of 2 4 dinitrophenol as small molecule model system |
| topic | 2,4-dinitrofenol Identificación Biosensores Biología Molecular Biosensors Identification Molecular Biology Superficies Au-MUA-PLL |
| url | http://hdl.handle.net/20.500.12123/2097 https://www.hindawi.com/journals/jnm/2016/5432656/ |
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