Inoculation of Native Arbuscular Mycorrhizae and Bacillus subtilis Can Improve Growth in Vegetable Crops
Arbuscular mycorrhizal fungi (AMF) and some rhizobacteria are known as plant growth-promoting microorganism (PGPM) as they play significant roles in improving soil fertility structure, plant nutrition, growth, and health. However, little is known about the PGPM potential of AMF and rhizobacteria nat...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Wiley
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/20963 https://onlinelibrary.wiley.com/doi/10.1155/2024/9226715 https://doi.org/10.1155/2024/9226715 |
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| author | Gebreslassie, Sara Jida, Mulissa Puente, Mariana Laura Covacevich, Fernanda Belay, Zerihun |
| author_browse | Belay, Zerihun Covacevich, Fernanda Gebreslassie, Sara Jida, Mulissa Puente, Mariana Laura |
| author_facet | Gebreslassie, Sara Jida, Mulissa Puente, Mariana Laura Covacevich, Fernanda Belay, Zerihun |
| author_sort | Gebreslassie, Sara |
| collection | INTA Digital |
| description | Arbuscular mycorrhizal fungi (AMF) and some rhizobacteria are known as plant growth-promoting microorganism (PGPM) as they play significant roles in improving soil fertility structure, plant nutrition, growth, and health. However, little is known about the PGPM potential of AMF and rhizobacteria native to the Rift Valley and highland regions of Ethiopia. Hence, this study aimed to investigate the PGPM effect of single and co-inoculation of AMF and the Bacillus subtilis ALCR46 strain, on tomato (Lycopersicum esculentum L.), onion (Allium cepa L.), and squash (Cucurbita pepo L.) plants. The experimental setup was a randomized complete block design with three replications of the following treatments: (i) inoculation with a consortium of AMF, (ii) co-inoculation with a consortium of AMF and the Bacillus subtilis, (iii) inoculation with Rhizophagus clarus, (iv) co-inoculation with R. clarus and B. subtilis, (v) inoculation with B. subtilis, (vi) plants without inoculation (negative control), and (vii) plants treated with chemical fertilizer (positive control). Plants were maintained in a greenhouse for 60 days, and after harvest, plant growth parameters, percentage of AMF root colonization, and spore number were analyzed. The result shows that the growth of crops significantly increased by co-inoculation with the consortium of AMF and B. subtilis. AMF spore density and root colonization rate were also increased in co-inoculated plants. Highest root colonization, spore number, and mycorrhizal dependency were observed in A. cepa. Our results suggest that there is a synergistic effect between the AMF and B. subtilis ALCR46, and between AMF inoculants. However, the application of present findings under field conditions is required to be confirmed by further studies. |
| format | info:ar-repo/semantics/artículo |
| id | INTA20963 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA209632025-01-10T17:04:06Z Inoculation of Native Arbuscular Mycorrhizae and Bacillus subtilis Can Improve Growth in Vegetable Crops Gebreslassie, Sara Jida, Mulissa Puente, Mariana Laura Covacevich, Fernanda Belay, Zerihun Bacillus subtilis Inoculación Microorganismos Promotores de Crecimiento Vegetal Rizobacteria Tomate Cebolla Micorriza Arbuscular Plant Growth-promoting Microorganisms Rhizobacteria Tomatoes Onions Arbuscular Mycorrhiza BPCV Calabazas Arbuscular mycorrhizal fungi (AMF) and some rhizobacteria are known as plant growth-promoting microorganism (PGPM) as they play significant roles in improving soil fertility structure, plant nutrition, growth, and health. However, little is known about the PGPM potential of AMF and rhizobacteria native to the Rift Valley and highland regions of Ethiopia. Hence, this study aimed to investigate the PGPM effect of single and co-inoculation of AMF and the Bacillus subtilis ALCR46 strain, on tomato (Lycopersicum esculentum L.), onion (Allium cepa L.), and squash (Cucurbita pepo L.) plants. The experimental setup was a randomized complete block design with three replications of the following treatments: (i) inoculation with a consortium of AMF, (ii) co-inoculation with a consortium of AMF and the Bacillus subtilis, (iii) inoculation with Rhizophagus clarus, (iv) co-inoculation with R. clarus and B. subtilis, (v) inoculation with B. subtilis, (vi) plants without inoculation (negative control), and (vii) plants treated with chemical fertilizer (positive control). Plants were maintained in a greenhouse for 60 days, and after harvest, plant growth parameters, percentage of AMF root colonization, and spore number were analyzed. The result shows that the growth of crops significantly increased by co-inoculation with the consortium of AMF and B. subtilis. AMF spore density and root colonization rate were also increased in co-inoculated plants. Highest root colonization, spore number, and mycorrhizal dependency were observed in A. cepa. Our results suggest that there is a synergistic effect between the AMF and B. subtilis ALCR46, and between AMF inoculants. However, the application of present findings under field conditions is required to be confirmed by further studies. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Gebreslassie, Sara. Adama Science and Technology University. Department of Applied Biology; Etiopía Fil: Jida, Mulissa. Bio and Emerging Technology Institute; Etiopía Fil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina Fil: Belay, Zerihun. Adama Science and Technology University. Department of Applied Biology; Etiopía 2025-01-10T16:52:48Z 2025-01-10T16:52:48Z 2024-05-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/20963 https://onlinelibrary.wiley.com/doi/10.1155/2024/9226715 1687-9198 1687-918X https://doi.org/10.1155/2024/9226715 eng info:eu-repograntAgreement/INTA/2023-PE-L04-I073, Desarrollo de bioinsumos y su integración en estrategias de manejo de adversidades bióticas y abióticas en cultivos agrícolas y forestales info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley International Journal of Microbiology : 9226715 (May 2024) |
| spellingShingle | Bacillus subtilis Inoculación Microorganismos Promotores de Crecimiento Vegetal Rizobacteria Tomate Cebolla Micorriza Arbuscular Plant Growth-promoting Microorganisms Rhizobacteria Tomatoes Onions Arbuscular Mycorrhiza BPCV Calabazas Gebreslassie, Sara Jida, Mulissa Puente, Mariana Laura Covacevich, Fernanda Belay, Zerihun Inoculation of Native Arbuscular Mycorrhizae and Bacillus subtilis Can Improve Growth in Vegetable Crops |
| title | Inoculation of Native Arbuscular Mycorrhizae and Bacillus subtilis Can Improve Growth in Vegetable Crops |
| title_full | Inoculation of Native Arbuscular Mycorrhizae and Bacillus subtilis Can Improve Growth in Vegetable Crops |
| title_fullStr | Inoculation of Native Arbuscular Mycorrhizae and Bacillus subtilis Can Improve Growth in Vegetable Crops |
| title_full_unstemmed | Inoculation of Native Arbuscular Mycorrhizae and Bacillus subtilis Can Improve Growth in Vegetable Crops |
| title_short | Inoculation of Native Arbuscular Mycorrhizae and Bacillus subtilis Can Improve Growth in Vegetable Crops |
| title_sort | inoculation of native arbuscular mycorrhizae and bacillus subtilis can improve growth in vegetable crops |
| topic | Bacillus subtilis Inoculación Microorganismos Promotores de Crecimiento Vegetal Rizobacteria Tomate Cebolla Micorriza Arbuscular Plant Growth-promoting Microorganisms Rhizobacteria Tomatoes Onions Arbuscular Mycorrhiza BPCV Calabazas |
| url | http://hdl.handle.net/20.500.12123/20963 https://onlinelibrary.wiley.com/doi/10.1155/2024/9226715 https://doi.org/10.1155/2024/9226715 |
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