Changes in micro RNA expression in a wild tuber-bearing Solanum species induced by 5-Azacytidine treatment
Phenotypic plasticity is often postulated as a principal characteristic of tuber-bearing wild Solanum species. The hypotheses to explore this observation have been developed based on the presence of genetic variation. In this context, evolutionary changes and adaptation are impossible without geneti...
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| Format: | Artículo |
| Language: | Inglés |
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2018
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| Online Access: | https://link.springer.com/article/10.1007%2Fs00299-012-1260-x http://hdl.handle.net/20.500.12123/2095 https://doi.org/10.1007/s00299-012-1260-x |
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| author | Marfil, Carlos Federico Asurmendi, Sebastian Masuelli, Ricardo Williams |
| author_browse | Asurmendi, Sebastian Marfil, Carlos Federico Masuelli, Ricardo Williams |
| author_facet | Marfil, Carlos Federico Asurmendi, Sebastian Masuelli, Ricardo Williams |
| author_sort | Marfil, Carlos Federico |
| collection | INTA Digital |
| description | Phenotypic plasticity is often postulated as a principal characteristic of tuber-bearing wild Solanum species. The hypotheses to explore this observation have been developed based on the presence of genetic variation. In this context, evolutionary changes and adaptation are impossible without genetic variation. However, epigenetic effects, which include DNA methylation and microRNAs expression control, could be another source of phenotypic variation in ecologically relevant traits. To achieve a detailed mechanistic understanding of these processes, it is necessary to separate epigenetic from DNA sequence-based effects and to evaluate their relative importance on phenotypic variability. We explored the potential relevance of epigenetic effects in individuals with the same genotype. For this purpose, a clone of the wild potato Solanum ruiz-lealii, a non-model species in which natural methylation variability has been demonstrated, was selected and its DNA methylation was manipulated applying 5-Azacytidine (AzaC), a demethylating agent. The AzaC treatment induced early flowering and changes in leaf morphology. Using quantitative real-time PCR, we identified four miRNAs up-regulated in the AzaC-treated plants. One of them, miRNA172, could play a role on the early flowering phenotype. In this work, we showed that the treatment with AzaC could provide meaningful results allowing to study both the phenotypic plasticity in tuber-bearing Solanum species and the inter-relation between DNA methylation and miRNA accumulations in a wide range of species. |
| format | Artículo |
| id | INTA2095 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA20952018-06-21T17:11:47Z Changes in micro RNA expression in a wild tuber-bearing Solanum species induced by 5-Azacytidine treatment Marfil, Carlos Federico Asurmendi, Sebastian Masuelli, Ricardo Williams Papa Plantas Silvestres ARN Genética Potatoes Wild Plants RNA Genetics Phenotypic plasticity is often postulated as a principal characteristic of tuber-bearing wild Solanum species. The hypotheses to explore this observation have been developed based on the presence of genetic variation. In this context, evolutionary changes and adaptation are impossible without genetic variation. However, epigenetic effects, which include DNA methylation and microRNAs expression control, could be another source of phenotypic variation in ecologically relevant traits. To achieve a detailed mechanistic understanding of these processes, it is necessary to separate epigenetic from DNA sequence-based effects and to evaluate their relative importance on phenotypic variability. We explored the potential relevance of epigenetic effects in individuals with the same genotype. For this purpose, a clone of the wild potato Solanum ruiz-lealii, a non-model species in which natural methylation variability has been demonstrated, was selected and its DNA methylation was manipulated applying 5-Azacytidine (AzaC), a demethylating agent. The AzaC treatment induced early flowering and changes in leaf morphology. Using quantitative real-time PCR, we identified four miRNAs up-regulated in the AzaC-treated plants. One of them, miRNA172, could play a role on the early flowering phenotype. In this work, we showed that the treatment with AzaC could provide meaningful results allowing to study both the phenotypic plasticity in tuber-bearing Solanum species and the inter-relation between DNA methylation and miRNA accumulations in a wide range of species. Instituto de Biotecnología Fil: Marfil, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina 2018-03-21T15:31:53Z 2018-03-21T15:31:53Z 2012-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007%2Fs00299-012-1260-x http://hdl.handle.net/20.500.12123/2095 0721-7714 1432-203X https://doi.org/10.1007/s00299-012-1260-x eng info:eu-repo/semantics/restrictedAccess application/pdf Plant cell reports 31 (8) : 1449–1461. (August 2012) |
| spellingShingle | Papa Plantas Silvestres ARN Genética Potatoes Wild Plants RNA Genetics Marfil, Carlos Federico Asurmendi, Sebastian Masuelli, Ricardo Williams Changes in micro RNA expression in a wild tuber-bearing Solanum species induced by 5-Azacytidine treatment |
| title | Changes in micro RNA expression in a wild tuber-bearing Solanum species induced by 5-Azacytidine treatment |
| title_full | Changes in micro RNA expression in a wild tuber-bearing Solanum species induced by 5-Azacytidine treatment |
| title_fullStr | Changes in micro RNA expression in a wild tuber-bearing Solanum species induced by 5-Azacytidine treatment |
| title_full_unstemmed | Changes in micro RNA expression in a wild tuber-bearing Solanum species induced by 5-Azacytidine treatment |
| title_short | Changes in micro RNA expression in a wild tuber-bearing Solanum species induced by 5-Azacytidine treatment |
| title_sort | changes in micro rna expression in a wild tuber bearing solanum species induced by 5 azacytidine treatment |
| topic | Papa Plantas Silvestres ARN Genética Potatoes Wild Plants RNA Genetics |
| url | https://link.springer.com/article/10.1007%2Fs00299-012-1260-x http://hdl.handle.net/20.500.12123/2095 https://doi.org/10.1007/s00299-012-1260-x |
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