Role of GPR55 receptor in bovine sperm capacitation
Background: Endocannabinoids like anandamide (AEA), among other lipids, are recognized signaling molecules that participate in reproductive events. Objectives: Our aims were to characterize orphan G protein-coupled receptor (GPR55) presence; investigate GPR55 activation by AEA and determine GPR55 r...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Wiley
2025
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/20895 https://onlinelibrary.wiley.com/doi/10.1111/andr.13823 https://doi.org/10.1111/andr.13823 |
| _version_ | 1855038320086614016 |
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| author | Lottero-Leconte, Raquel Lara, Angela Plaza, Jessica Arroyo-Salvo, Camila Bogetti, María Eugenia Ynsaurralde Rivolta, Amanda Eugenia Dellavalle, Franco Alejandro Sengiali, Fiamma Cetica, Pablo Rio, Sofía Zalazar, Lucia Cesari, Andreína Miragaya, Marcelo Morado, Sergio Perez-Martinez, Silvina |
| author_browse | Arroyo-Salvo, Camila Bogetti, María Eugenia Cesari, Andreína Cetica, Pablo Dellavalle, Franco Alejandro Lara, Angela Lottero-Leconte, Raquel Miragaya, Marcelo Morado, Sergio Perez-Martinez, Silvina Plaza, Jessica Rio, Sofía Sengiali, Fiamma Ynsaurralde Rivolta, Amanda Eugenia Zalazar, Lucia |
| author_facet | Lottero-Leconte, Raquel Lara, Angela Plaza, Jessica Arroyo-Salvo, Camila Bogetti, María Eugenia Ynsaurralde Rivolta, Amanda Eugenia Dellavalle, Franco Alejandro Sengiali, Fiamma Cetica, Pablo Rio, Sofía Zalazar, Lucia Cesari, Andreína Miragaya, Marcelo Morado, Sergio Perez-Martinez, Silvina |
| author_sort | Lottero-Leconte, Raquel |
| collection | INTA Digital |
| description | Background: Endocannabinoids like anandamide (AEA), among other lipids, are recognized signaling molecules that participate in reproductive events.
Objectives: Our aims were to characterize orphan G protein-coupled receptor (GPR55) presence; investigate GPR55 activation by AEA and determine GPR55 role in the bovine sperm function.
Materials and methods: GPR55 presence was assessed by immunocytochemistry. Protein kinase A (pPKA) and PKC (pPKC) substrates, pERK1/2, G/F-actin were determined by Western blotting, activation of RAC-1 by pull-down assay, F-actin and acrosomal exocytosis by fluorescence microscopy, sperm motility by optic microscopy and computer-aided sperm analysis and fertilizing ability by in vitro fertilization (IVF).
Results: We detected GPR55 in spermatozoa at T0, after incubation in non-capacitating and capacitating (presence of AEA) conditions and upon release from oviductal epithelia. AEA induced an increase in pPKA and pPKC, while CID16020046 (CID), selective GPR55 antagonist, prevented this effect. Incubation with H89, PKA inhibitor, significantly decreased pPKC, while Gö6983, a PKC inhibitor, partially reduced pPKA. pPKA remained elevated at 15- and 45-min incubation, while pPKC decreased at 15 and increased at 45 min. CID prevented pPKC increase at 5 and 45 min and decreased pPKA at 45 min. RAC-1 and F-actin increase induced by AEA was prevented by CID. Variations in two progressive motility kinematic parameters were observed with AEA and/or CID. Sperm pretreatment with AEA increased the rate of cleaved embryos and CID prevented this effect.
Discussion: We demonstrated that GPR55 activation by AEA induces time-dependent signaling pathways involving pPKA and pPKC during bovine sperm capacitation. AEA regulates actin polymerization through GPR55 activation, suggesting the receptor participates in cytoskeleton remodeling, and yielded higher IVF rates. Also, sperm pre-incubation with molecules like AEA involved in capacitation could improve the embryo development.
Conclusion: We have demonstrated GPR55 presence in bovine spermatozoa. The regulation of PKA and PKC and of molecules associated with cytoskeletal dynamics, such as RAC-1 and actin, by GPR55 is closely related to sperm motility and acrosomal exocytosis. |
| format | info:ar-repo/semantics/artículo |
| id | INTA20895 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA208952025-01-08T11:36:04Z Role of GPR55 receptor in bovine sperm capacitation Lottero-Leconte, Raquel Lara, Angela Plaza, Jessica Arroyo-Salvo, Camila Bogetti, María Eugenia Ynsaurralde Rivolta, Amanda Eugenia Dellavalle, Franco Alejandro Sengiali, Fiamma Cetica, Pablo Rio, Sofía Zalazar, Lucia Cesari, Andreína Miragaya, Marcelo Morado, Sergio Perez-Martinez, Silvina Ganado Bovino Espermatozoo Reproducción Animal Cattle Spermatozoa Animal Reproduction Esperma Sperm Background: Endocannabinoids like anandamide (AEA), among other lipids, are recognized signaling molecules that participate in reproductive events. Objectives: Our aims were to characterize orphan G protein-coupled receptor (GPR55) presence; investigate GPR55 activation by AEA and determine GPR55 role in the bovine sperm function. Materials and methods: GPR55 presence was assessed by immunocytochemistry. Protein kinase A (pPKA) and PKC (pPKC) substrates, pERK1/2, G/F-actin were determined by Western blotting, activation of RAC-1 by pull-down assay, F-actin and acrosomal exocytosis by fluorescence microscopy, sperm motility by optic microscopy and computer-aided sperm analysis and fertilizing ability by in vitro fertilization (IVF). Results: We detected GPR55 in spermatozoa at T0, after incubation in non-capacitating and capacitating (presence of AEA) conditions and upon release from oviductal epithelia. AEA induced an increase in pPKA and pPKC, while CID16020046 (CID), selective GPR55 antagonist, prevented this effect. Incubation with H89, PKA inhibitor, significantly decreased pPKC, while Gö6983, a PKC inhibitor, partially reduced pPKA. pPKA remained elevated at 15- and 45-min incubation, while pPKC decreased at 15 and increased at 45 min. CID prevented pPKC increase at 5 and 45 min and decreased pPKA at 45 min. RAC-1 and F-actin increase induced by AEA was prevented by CID. Variations in two progressive motility kinematic parameters were observed with AEA and/or CID. Sperm pretreatment with AEA increased the rate of cleaved embryos and CID prevented this effect. Discussion: We demonstrated that GPR55 activation by AEA induces time-dependent signaling pathways involving pPKA and pPKC during bovine sperm capacitation. AEA regulates actin polymerization through GPR55 activation, suggesting the receptor participates in cytoskeleton remodeling, and yielded higher IVF rates. Also, sperm pre-incubation with molecules like AEA involved in capacitation could improve the embryo development. Conclusion: We have demonstrated GPR55 presence in bovine spermatozoa. The regulation of PKA and PKC and of molecules associated with cytoskeletal dynamics, such as RAC-1 and actin, by GPR55 is closely related to sperm motility and acrosomal exocytosis. EEA Mercedes Fil: Lottero-Leconte, Raquel. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos (CEFYBO); Argentina Fil: Lottero-Leconte, Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Farmacológicos y Botánicos (CEFYBO); Argentina Fil: Lara, Angela. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos (CEFYBO); Argentina Fil: Lara, Angela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Farmacológicos y Botánicos (CEFYBO); Argentina Fil: Plaza, Jessica. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA); Argentina Fil: Arroyo-Salvo, Camila. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos (CEFYBO); Argentina Fil: Arroyo-Salvo, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Farmacológicos y Botánicos (CEFYBO); Argentina Fil: Bogetti, María Eugenia. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos (CEFYBO); Argentina Fil: Bogetti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Farmacológicos y Botánicos (CEFYBO); Argentina Fil: Ynsaurralde Rivolta, Amada Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina. Fil: Dellavalle, Franco A. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina. Fil: Sengiali, Fiamma. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA); Argentina Fil: Cetica, Pablo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA); Argentina Fil: Cetica, Pablo. Universidad de Buenos Aires. Instituto de Investigaciones en Producción Animal (INPA); Argentina Fil: Cetica, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Producción Animal (INPA); Argentina Fil: Rio, Sofía. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos (CEFYBO); Argentina Fil: Rio, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Farmacológicos y Botánicos (CEFYBO); Argentina Fil: Zalazar, Lucia. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Biológicas; Argentina Fil: Zalazar, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas; Argentina Fil: Cesari, Andreína. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Biológicas; Argentina Fil: Cesari, Andreína. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas; Argentina Fil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA); Argentina Fil: Morado, Sergio. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal (INITRA); Argentina Fil: Morado, Sergio. Universidad de Buenos Aires. Instituto de Investigaciones en Producción Animal (INPA); Argentina Fil: Morado, Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Producción Animal (INPA); Argentina Fil: Perez-Martinez, Silvina. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos (CEFYBO); Argentina Fil: Perez-Martinez, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Farmacológicos y Botánicos (CEFYBO); Argentina 2025-01-08T11:33:51Z 2025-01-08T11:33:51Z 2025-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/20895 https://onlinelibrary.wiley.com/doi/10.1111/andr.13823 2047-2919 2047-2927 https://doi.org/10.1111/andr.13823 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Andrology : 1-18. (First published: 03 January 2025) |
| spellingShingle | Ganado Bovino Espermatozoo Reproducción Animal Cattle Spermatozoa Animal Reproduction Esperma Sperm Lottero-Leconte, Raquel Lara, Angela Plaza, Jessica Arroyo-Salvo, Camila Bogetti, María Eugenia Ynsaurralde Rivolta, Amanda Eugenia Dellavalle, Franco Alejandro Sengiali, Fiamma Cetica, Pablo Rio, Sofía Zalazar, Lucia Cesari, Andreína Miragaya, Marcelo Morado, Sergio Perez-Martinez, Silvina Role of GPR55 receptor in bovine sperm capacitation |
| title | Role of GPR55 receptor in bovine sperm capacitation |
| title_full | Role of GPR55 receptor in bovine sperm capacitation |
| title_fullStr | Role of GPR55 receptor in bovine sperm capacitation |
| title_full_unstemmed | Role of GPR55 receptor in bovine sperm capacitation |
| title_short | Role of GPR55 receptor in bovine sperm capacitation |
| title_sort | role of gpr55 receptor in bovine sperm capacitation |
| topic | Ganado Bovino Espermatozoo Reproducción Animal Cattle Spermatozoa Animal Reproduction Esperma Sperm |
| url | http://hdl.handle.net/20.500.12123/20895 https://onlinelibrary.wiley.com/doi/10.1111/andr.13823 https://doi.org/10.1111/andr.13823 |
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