A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply
We previously reported a structural rearrangement between wheat (Triticum aestivum) and rye (Secale c ereale) chromosomes 1BS/1RS that increased the dosage of 12-OXOPHYTODIENOATE REDUCTASE III (OPRIII) genes involved in jasmonate biosynthesis (henceforth, 1RW line), and that drastically reduced prim...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2024
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/20751 https://www.sciencedirect.com/science/article/pii/S0176161724002074 https://doi.org/10.1016/j.jplph.2024.154376 |
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| author | Gualano, Leonardo D. Moriconi, Jorge I. Gabay, Gilad Tranquilli, Gabriela Pacheco, Pablo H. Dubcovsky, Jorge Santa-María, Guillermo E. |
| author_browse | Dubcovsky, Jorge Gabay, Gilad Gualano, Leonardo D. Moriconi, Jorge I. Pacheco, Pablo H. Santa-María, Guillermo E. Tranquilli, Gabriela |
| author_facet | Gualano, Leonardo D. Moriconi, Jorge I. Gabay, Gilad Tranquilli, Gabriela Pacheco, Pablo H. Dubcovsky, Jorge Santa-María, Guillermo E. |
| author_sort | Gualano, Leonardo D. |
| collection | INTA Digital |
| description | We previously reported a structural rearrangement between wheat (Triticum aestivum) and rye (Secale c ereale) chromosomes 1BS/1RS that increased the dosage of 12-OXOPHYTODIENOATE REDUCTASE III (OPRIII) genes involved in jasmonate biosynthesis (henceforth, 1RW line), and that drastically reduced primary root growth relative to a control line with the intact 1RS chromosome (henceforth, 1RS). In this study, we show that the increased gene-dosage of this region is associated with increases in the shoot-root partitioning of magnesium (Mg). Moreover, both a CRISPR-edited 1RW line with reduced OPRIII dosage and the 1RW line treated with the jasmonate biosynthesis inhibitor ibuprofen showed reduced differences in shoot-root Mg partitioning than 1RW.
The observed differences in Mg partitioning between 1RS and 1RW plants occur over a wide range of external Mg supplies and imply opposite trends of Mg accumulation in roots and shoots. Furthermore, we show an association between the increase of shoot-root Mg partitioning and increased tolerance of the 1RW line to low levels of Mg supply. In summary, our results provide evidence of the role of the jasmonate pathway on the dynamics of Mg accumulation in roots and shoots, which correlates with the performance of wheat plants under conditions of Mg scarcity. |
| format | Artículo |
| id | INTA20751 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA207512024-12-26T12:16:13Z A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply Gualano, Leonardo D. Moriconi, Jorge I. Gabay, Gilad Tranquilli, Gabriela Pacheco, Pablo H. Dubcovsky, Jorge Santa-María, Guillermo E. Jasmonates Magnesium Rye Wheat Jasmonato Magnesio Centeno Trigo 12-OXOPHYTODIENOATE REDUCTASE III 12-OXOFITODIENOATO REDUCTASA III We previously reported a structural rearrangement between wheat (Triticum aestivum) and rye (Secale c ereale) chromosomes 1BS/1RS that increased the dosage of 12-OXOPHYTODIENOATE REDUCTASE III (OPRIII) genes involved in jasmonate biosynthesis (henceforth, 1RW line), and that drastically reduced primary root growth relative to a control line with the intact 1RS chromosome (henceforth, 1RS). In this study, we show that the increased gene-dosage of this region is associated with increases in the shoot-root partitioning of magnesium (Mg). Moreover, both a CRISPR-edited 1RW line with reduced OPRIII dosage and the 1RW line treated with the jasmonate biosynthesis inhibitor ibuprofen showed reduced differences in shoot-root Mg partitioning than 1RW. The observed differences in Mg partitioning between 1RS and 1RW plants occur over a wide range of external Mg supplies and imply opposite trends of Mg accumulation in roots and shoots. Furthermore, we show an association between the increase of shoot-root Mg partitioning and increased tolerance of the 1RW line to low levels of Mg supply. In summary, our results provide evidence of the role of the jasmonate pathway on the dynamics of Mg accumulation in roots and shoots, which correlates with the performance of wheat plants under conditions of Mg scarcity. Instituto de Recursos Biológicos Fil: Gualano, Leonardo D. Universidad Nacional de San Martín. Escuela de Bio y Nanotecnologías. Instituto Tecnológico Chascomús (INTECH); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil Moriconi, Jorge I. Universidad Nacional de San Martín. Escuela de Bio y Nanotecnologías. Instituto Tecnológico Chascomús (INTECH); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gabay, Gilad. University of California. Department of Plant Sciences; Estados Unidos Fil: Tranquilli, Gabriela E. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Pacheco, Pablo H. Universidad Nacional de San Luís. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luís (INQUISAL); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Duvcoscky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos Fil: Santa María, Guillermo E. Universidad Nacional de San Martín. Escuela de Bio y Nanotecnologías. Instituto Tecnológico Chascomús (INTECH); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-12-26T12:03:12Z 2024-12-26T12:03:12Z 2024-11-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/20751 https://www.sciencedirect.com/science/article/pii/S0176161724002074 0176-1617 https://doi.org/10.1016/j.jplph.2024.154376 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Journal of Plant Physiology 303 : 154376. (December 2024) |
| spellingShingle | Jasmonates Magnesium Rye Wheat Jasmonato Magnesio Centeno Trigo 12-OXOPHYTODIENOATE REDUCTASE III 12-OXOFITODIENOATO REDUCTASA III Gualano, Leonardo D. Moriconi, Jorge I. Gabay, Gilad Tranquilli, Gabriela Pacheco, Pablo H. Dubcovsky, Jorge Santa-María, Guillermo E. A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply |
| title | A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply |
| title_full | A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply |
| title_fullStr | A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply |
| title_full_unstemmed | A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply |
| title_short | A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply |
| title_sort | triplicated wheat rye chromosome segment including several 12 oxophytodienoate reductase iii genes influences magnesium partitioning and impacts wheat performance at low magnesium supply |
| topic | Jasmonates Magnesium Rye Wheat Jasmonato Magnesio Centeno Trigo 12-OXOPHYTODIENOATE REDUCTASE III 12-OXOFITODIENOATO REDUCTASA III |
| url | http://hdl.handle.net/20.500.12123/20751 https://www.sciencedirect.com/science/article/pii/S0176161724002074 https://doi.org/10.1016/j.jplph.2024.154376 |
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