Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic Sequence
The genome of Bacillus thuringiensis strain INTA 103-23 was sequenced, revealing a high-quality draft assembly comprising 243 contigs with a total size of 6.30 Mb and a completeness of 99%. Phylogenetic analysis classified INTA 103-23 within the Bacillus cereus sensu stricto cluster. Genome annotati...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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MDPI
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/20529 https://www.mdpi.com/2306-5729/9/3/40 https://doi.org/10.3390/data9030040 |
| _version_ | 1855038245722652672 |
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| author | Palma, Leopoldo Ortiz, Leila Melina Niz, José María Berretta, Marcelo Facundo Sauka, Diego Hernan |
| author_browse | Berretta, Marcelo Facundo Niz, José María Ortiz, Leila Melina Palma, Leopoldo Sauka, Diego Hernan |
| author_facet | Palma, Leopoldo Ortiz, Leila Melina Niz, José María Berretta, Marcelo Facundo Sauka, Diego Hernan |
| author_sort | Palma, Leopoldo |
| collection | INTA Digital |
| description | The genome of Bacillus thuringiensis strain INTA 103-23 was sequenced, revealing a high-quality draft assembly comprising 243 contigs with a total size of 6.30 Mb and a completeness of 99%. Phylogenetic analysis classified INTA 103-23 within the Bacillus cereus sensu stricto cluster. Genome annotation identified 6993 genes, including 2476 hypothetical proteins. Screening for pesticidal proteins unveiled 10 coding sequences with significant similarity to known pesticidal proteins, showcasing a potential efficacy against various insect orders. AntiSMASH analysis predicted 13 biosynthetic gene clusters (BGCs), including clusters with 100% similarity to petrobactin and anabaenopeptin NZ857/nostamide A. Notably, fengycin exhibited a 40% similarity within the identified clusters. Further exploration involved a comparative genomic analysis with ten phylogenetically closest genomes. The ANI values, calculated using fastANI, confirmed the closest relationships with strains classified under Bacillus cereus sensu stricto. This comprehensive genomic analysis of B. thuringiensis INTA 103-23 provides valuable insights into its genetic makeup, potential pesticidal activity, and biosynthetic capabilities. The identified BGCs and pesticidal proteins contribute to our understanding of the strain’s biocontrol potential against diverse agricultural pests. |
| format | info:ar-repo/semantics/artículo |
| id | INTA20529 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA205292024-12-09T14:42:13Z Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic Sequence Palma, Leopoldo Ortiz, Leila Melina Niz, José María Berretta, Marcelo Facundo Sauka, Diego Hernan Control Biológico Plagas Insecticidas Pests Biological Control Insecticides Bacillus thuringiensis Biovar Thuringiensis Insecticidal Proteins Genome Sequence The genome of Bacillus thuringiensis strain INTA 103-23 was sequenced, revealing a high-quality draft assembly comprising 243 contigs with a total size of 6.30 Mb and a completeness of 99%. Phylogenetic analysis classified INTA 103-23 within the Bacillus cereus sensu stricto cluster. Genome annotation identified 6993 genes, including 2476 hypothetical proteins. Screening for pesticidal proteins unveiled 10 coding sequences with significant similarity to known pesticidal proteins, showcasing a potential efficacy against various insect orders. AntiSMASH analysis predicted 13 biosynthetic gene clusters (BGCs), including clusters with 100% similarity to petrobactin and anabaenopeptin NZ857/nostamide A. Notably, fengycin exhibited a 40% similarity within the identified clusters. Further exploration involved a comparative genomic analysis with ten phylogenetically closest genomes. The ANI values, calculated using fastANI, confirmed the closest relationships with strains classified under Bacillus cereus sensu stricto. This comprehensive genomic analysis of B. thuringiensis INTA 103-23 provides valuable insights into its genetic makeup, potential pesticidal activity, and biosynthetic capabilities. The identified BGCs and pesticidal proteins contribute to our understanding of the strain’s biocontrol potential against diverse agricultural pests. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Palma, Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Palma, Leopoldo. Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB-CONICET); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB-CONICET); Argentina. Fil: Palma, Leopoldo. Universitat de València, Departamento de Genética. Instituto BIOTECMED, Laboratorio de Control Biotecnológico de Plagas; España Fil: Ortiz, Leila Melina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Niz, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Berretta, Marcelo Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-12-09T14:30:21Z 2024-12-09T14:30:21Z 2024-02-28 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/20529 https://www.mdpi.com/2306-5729/9/3/40 2306-5729 https://doi.org/10.3390/data9030040 eng info:eu-repograntAgreement/INTA/2023-PD-L01-I087, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada. info:eu-repograntAgreement/INTA/2023-PE-L04-I073, Desarrollo de bioinsumos y su integración en estrategias de manejo de adversidades bióticas y abióticas en cultivos agrícolas y forestales info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Data 9 (3) : 40 (February 2024) |
| spellingShingle | Control Biológico Plagas Insecticidas Pests Biological Control Insecticides Bacillus thuringiensis Biovar Thuringiensis Insecticidal Proteins Genome Sequence Palma, Leopoldo Ortiz, Leila Melina Niz, José María Berretta, Marcelo Facundo Sauka, Diego Hernan Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic Sequence |
| title | Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic Sequence |
| title_full | Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic Sequence |
| title_fullStr | Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic Sequence |
| title_full_unstemmed | Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic Sequence |
| title_short | Draft Genome Sequence of Bacillus thuringiensis INTA 103-23 Reveals Its Insecticidal Properties: Insights from the Genomic Sequence |
| title_sort | draft genome sequence of bacillus thuringiensis inta 103 23 reveals its insecticidal properties insights from the genomic sequence |
| topic | Control Biológico Plagas Insecticidas Pests Biological Control Insecticides Bacillus thuringiensis Biovar Thuringiensis Insecticidal Proteins Genome Sequence |
| url | http://hdl.handle.net/20.500.12123/20529 https://www.mdpi.com/2306-5729/9/3/40 https://doi.org/10.3390/data9030040 |
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