In silico evaluation of Lipl32 peptides for antigen candidates in leptospirosis diagnostic
Poster
| Main Authors: | , , , , , , |
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| Format: | Conferencia |
| Language: | Inglés |
| Published: |
International Leptospirosis Society
2024
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/20487 |
| _version_ | 1855486580604534784 |
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| author | Esteban, Micaela Saraullo, Vanina Rosa Madero, Agustin Hamer, Micaela Sanchez, Maria Cristina Brihuega, Bibiana Felicitas Martinez, Mara Leila |
| author_browse | Brihuega, Bibiana Felicitas Esteban, Micaela Hamer, Micaela Madero, Agustin Martinez, Mara Leila Sanchez, Maria Cristina Saraullo, Vanina Rosa |
| author_facet | Esteban, Micaela Saraullo, Vanina Rosa Madero, Agustin Hamer, Micaela Sanchez, Maria Cristina Brihuega, Bibiana Felicitas Martinez, Mara Leila |
| author_sort | Esteban, Micaela |
| collection | INTA Digital |
| description | Poster |
| format | Conferencia |
| id | INTA20487 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | International Leptospirosis Society |
| publisherStr | International Leptospirosis Society |
| record_format | dspace |
| spelling | INTA204872024-12-05T11:41:56Z In silico evaluation of Lipl32 peptides for antigen candidates in leptospirosis diagnostic Esteban, Micaela Saraullo, Vanina Rosa Madero, Agustin Hamer, Micaela Sanchez, Maria Cristina Brihuega, Bibiana Felicitas Martinez, Mara Leila Peptides Antigens Leptospirosis Immunodiagnosis Péptidos Antígenos Inmunodiagnóstico Poster Leptospirosis is a zoonotic infectious disease caused by the spirochete of the genus Leptospira. The gold-standard technique for diagnosis is the microscopic agglutina tion test, however, this serological test is complex and time consuming, and requires specific equipment, highly trained staff, and the live cultures of reference strains of Leptospira for use as antigens. It is for this reason that the search for antigenic can didates for immunodiagnostics takes on significant importance. LipL32 is an outer membrane lipoprotein that is exclusively found in pathogenic leptospires, highly con served and immunogenic. In this study, in silico analysis of the amino acid sequence of LipL32 was carried out with the aim of finding peptides that are good candidates for diagnostic use, bound to gold nanoparticles. First, amino acid sequences of lipL32 were searched in Genbank and conservation in pathogenic leptospires (Pomona and Hardjo) was compared between them, using the MUSCLE clustal platform. The signal peptide region was determined by DTU health tech as it is ruled out as an anti gen. Remaining amino acid sequence was evaluated in different predictors of epi topes (EMBOSS, Antibody Epitope Prediction, SVMTrip, Predicted Antigenic Peptides, ABC Pred). Once the results of the predictors were obtained, they were compared with each other. The peptides that matched in most of the predictors were selected and evaluated with PepCalc for molecular weight and water solubility. Instituto de Patobiología Fil: Esteban, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Esteban, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Madero, Agustin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Madero, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sanchez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Sanchez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brihuega, Bibiana Felicitas. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias. Instituto de Investigación en Veterinaria; Argentina Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martinez, Mara Leila. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias. Instituto de Investigación en Veterinaria; Argentina 2024-12-05T11:34:21Z 2024-12-05T11:34:21Z 2024-09 info:ar-repo/semantics/documento de conferencia info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/20487 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf International Leptospirosis Society The 13th Conference of the International Leptospirosis Society and The 4th Meeting of the European Leptospirosis and other Roden-Born Haemorrhagic fevers Society, Brussels, 2-4 September 2024 |
| spellingShingle | Peptides Antigens Leptospirosis Immunodiagnosis Péptidos Antígenos Inmunodiagnóstico Esteban, Micaela Saraullo, Vanina Rosa Madero, Agustin Hamer, Micaela Sanchez, Maria Cristina Brihuega, Bibiana Felicitas Martinez, Mara Leila In silico evaluation of Lipl32 peptides for antigen candidates in leptospirosis diagnostic |
| title | In silico evaluation of Lipl32 peptides for antigen candidates in leptospirosis diagnostic |
| title_full | In silico evaluation of Lipl32 peptides for antigen candidates in leptospirosis diagnostic |
| title_fullStr | In silico evaluation of Lipl32 peptides for antigen candidates in leptospirosis diagnostic |
| title_full_unstemmed | In silico evaluation of Lipl32 peptides for antigen candidates in leptospirosis diagnostic |
| title_short | In silico evaluation of Lipl32 peptides for antigen candidates in leptospirosis diagnostic |
| title_sort | in silico evaluation of lipl32 peptides for antigen candidates in leptospirosis diagnostic |
| topic | Peptides Antigens Leptospirosis Immunodiagnosis Péptidos Antígenos Inmunodiagnóstico |
| url | http://hdl.handle.net/20.500.12123/20487 |
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