Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in Argentina
During a survey conducted in the 2021, 2022 and 2023 seasons, sunflower heads showing symptoms of dry rot were collected from 80 fields in the semi-arid Pampa region of Argentina. A total of 72% of the fields examined in 2021, 71% in 2022 and 63% in 2023 showed the presence of the disease. The highe...
| Main Authors: | , , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Wiley
2024
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/20452 https://onlinelibrary.wiley.com/doi/10.1111/jph.13416 https://doi.org/10.1111/jph.13416 |
| _version_ | 1855038230937731072 |
|---|---|
| author | Colombo, Denis Nahuel Figueruelo, Andrea Mariana Paniego, Norma Beatriz Comerio, Ricardo Mario Corro Molas, Andres Ezequiel |
| author_browse | Colombo, Denis Nahuel Comerio, Ricardo Mario Corro Molas, Andres Ezequiel Figueruelo, Andrea Mariana Paniego, Norma Beatriz |
| author_facet | Colombo, Denis Nahuel Figueruelo, Andrea Mariana Paniego, Norma Beatriz Comerio, Ricardo Mario Corro Molas, Andres Ezequiel |
| author_sort | Colombo, Denis Nahuel |
| collection | INTA Digital |
| description | During a survey conducted in the 2021, 2022 and 2023 seasons, sunflower heads showing symptoms of dry rot were collected from 80 fields in the semi-arid Pampa region of Argentina. A total of 72% of the fields examined in 2021, 71% in 2022 and 63% in 2023 showed the presence of the disease. The highest incidence was recorded as 70% in 2021. Phomopsis head rot began as dry, brown spots on the back of the head, progressing to necrosis and twisting of adjacent leaves. Infected head tissue samples were surface sterilised, cultured on potato dextrose agar (PDA) and incubated at 25°C for 14 days. Diaporthe helianthi was identified based on cultural and morphological characteristics as well as molecular data. A phylogenetic analysis was performed. Internal transcribed spacer (ITS), translation elongation factor 1-α (ef1-α) and β-tubulin sequences were deposited in GenBank, showing the identity with the ex-type D. helianthi strain CBS 592.81. Pathogenicity experiments confirmed the presence of similar disease symptoms in inoculated sunflower heads, and D. helianthi was consistently reisolated from these organs. Our Koch's postulates testing results on heads constitute the first confirmed report that D. helianthi is the cause of Phomopsis head rot on sunflower in Argentina. |
| format | info:ar-repo/semantics/artículo |
| id | INTA20452 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA204522024-12-03T13:53:27Z Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in Argentina Colombo, Denis Nahuel Figueruelo, Andrea Mariana Paniego, Norma Beatriz Comerio, Ricardo Mario Corro Molas, Andres Ezequiel Disease Recognition Fungi Phylogenetic Analysis Phomopsis Diaporthe helianthi Sunflowers Reconocimiento de la Enfermedad Hongos Análisis Filogenético Girasol Argentina During a survey conducted in the 2021, 2022 and 2023 seasons, sunflower heads showing symptoms of dry rot were collected from 80 fields in the semi-arid Pampa region of Argentina. A total of 72% of the fields examined in 2021, 71% in 2022 and 63% in 2023 showed the presence of the disease. The highest incidence was recorded as 70% in 2021. Phomopsis head rot began as dry, brown spots on the back of the head, progressing to necrosis and twisting of adjacent leaves. Infected head tissue samples were surface sterilised, cultured on potato dextrose agar (PDA) and incubated at 25°C for 14 days. Diaporthe helianthi was identified based on cultural and morphological characteristics as well as molecular data. A phylogenetic analysis was performed. Internal transcribed spacer (ITS), translation elongation factor 1-α (ef1-α) and β-tubulin sequences were deposited in GenBank, showing the identity with the ex-type D. helianthi strain CBS 592.81. Pathogenicity experiments confirmed the presence of similar disease symptoms in inoculated sunflower heads, and D. helianthi was consistently reisolated from these organs. Our Koch's postulates testing results on heads constitute the first confirmed report that D. helianthi is the cause of Phomopsis head rot on sunflower in Argentina. Instituto de Biotecnología Fil: Colombo, Denis Nahuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina Fil: Figueruelo, Andrea Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina Fil: Figueruelo, Andrea Mariana. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Paniego, Norma Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Comerio, Ricardo Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina Fil: Corro Molas, Andres Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina Fil: Corro Molas, Andres Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil. Agencia de Extensión Rural General Pico; Argentina 2024-12-03T13:39:09Z 2024-12-03T13:39:09Z 2024-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/20452 https://onlinelibrary.wiley.com/doi/10.1111/jph.13416 1439-0434 https://doi.org/10.1111/jph.13416 eng info:eu-repograntAgreement/INTA/2019-PD-E4-I090-001, Análisis de patosistemas en cultivos agrícolas y especies forestales. Caracterización de sus componentes info:eu-repograntAgreement/INTA/2019-PE-E6-I114-001, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada info:eu-repograntAgreement/INTA/2023-PE-L01-I111, Mejoramiento genético de oleaginosas: girasol, soja, colza y lino en rendimiento, calidad y sanidad, para contribuir a la sostenibilidad de los sistemas productivos info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Journal of Phytopathology 172 (5) : e13416 (September/October 2024) |
| spellingShingle | Disease Recognition Fungi Phylogenetic Analysis Phomopsis Diaporthe helianthi Sunflowers Reconocimiento de la Enfermedad Hongos Análisis Filogenético Girasol Argentina Colombo, Denis Nahuel Figueruelo, Andrea Mariana Paniego, Norma Beatriz Comerio, Ricardo Mario Corro Molas, Andres Ezequiel Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in Argentina |
| title | Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in Argentina |
| title_full | Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in Argentina |
| title_fullStr | Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in Argentina |
| title_full_unstemmed | Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in Argentina |
| title_short | Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in Argentina |
| title_sort | phomopsis head rot caused by diaporthe helianthi a new disease on sunflower in argentina |
| topic | Disease Recognition Fungi Phylogenetic Analysis Phomopsis Diaporthe helianthi Sunflowers Reconocimiento de la Enfermedad Hongos Análisis Filogenético Girasol Argentina |
| url | http://hdl.handle.net/20.500.12123/20452 https://onlinelibrary.wiley.com/doi/10.1111/jph.13416 https://doi.org/10.1111/jph.13416 |
| work_keys_str_mv | AT colombodenisnahuel phomopsisheadrotcausedbydiaporthehelianthianewdiseaseonsunflowerinargentina AT figuerueloandreamariana phomopsisheadrotcausedbydiaporthehelianthianewdiseaseonsunflowerinargentina AT paniegonormabeatriz phomopsisheadrotcausedbydiaporthehelianthianewdiseaseonsunflowerinargentina AT comerioricardomario phomopsisheadrotcausedbydiaporthehelianthianewdiseaseonsunflowerinargentina AT corromolasandresezequiel phomopsisheadrotcausedbydiaporthehelianthianewdiseaseonsunflowerinargentina |