Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium
Cathelicidin are innate antimicrobial peptides with broad immunomodulatory functions; however, their role in regulating intestinal defenses is not well characterized. This study aimed to investigate the role of cathelicidin modulating expression of Toll-like receptors (TLRs) 4 and 9 in colonic epith...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/2018 https://www.sciencedirect.com/science/article/pii/S0161589017305102?via%3Dihub#! https://doi.org/10.1016/j.molimm.2017.09.011 |
| _version_ | 1855482980805378048 |
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| author | Marín, Maia Solange Holani, Ravi Shah, Chaitanya B. Odeon, Anselmo Carlos Cobo, Eduardo Rubén |
| author_browse | Cobo, Eduardo Rubén Holani, Ravi Marín, Maia Solange Odeon, Anselmo Carlos Shah, Chaitanya B. |
| author_facet | Marín, Maia Solange Holani, Ravi Shah, Chaitanya B. Odeon, Anselmo Carlos Cobo, Eduardo Rubén |
| author_sort | Marín, Maia Solange |
| collection | INTA Digital |
| description | Cathelicidin are innate antimicrobial peptides with broad immunomodulatory functions; however, their role in regulating intestinal defenses is not well characterized. This study aimed to investigate the role of cathelicidin modulating expression of Toll-like receptors (TLRs) 4 and 9 in colonic epithelium in response to bacterial patterns. We demonstrated herein that intestinal epithelial cells, when primed by bacterial lipopolysaccharide (LPS), responded to cathelicidin by increased transcription and protein synthesis of TLR4. This cathelicidin-induced response required the interaction of LPS-TLR4 and activation of MAPK signalling pathways. However, cathelicidin blocked TLR9 responses induced by TLR9 ligand CpG oligodeoxynucleotide (CpG ODN) in these colonic epithelial cells. Modulations of TLRs triggered by cathelicidin in intestinal epithelium occurred mainly in the apical compartment of intestinal cells. Activation of TLR4 by ligands in combination with cathelicidin promoted CXCL8 chemokine secretion and epithelial antimicrobial defenses against Escherichia coli. We concluded that cathelicidin selectively modulated synthesis of TLR4 and 9 in intestinal epithelium, but only when cells were exposed to virulence factors, mostly from apical surfaces. Enhanced TLR4 expression promoted by cathelicidin in intestinal epithelium may be crucial for controlling enteric infectious diseases. |
| format | Artículo |
| id | INTA2018 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA20182018-08-24T18:25:31Z Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium Marín, Maia Solange Holani, Ravi Shah, Chaitanya B. Odeon, Anselmo Carlos Cobo, Eduardo Rubén Enfermedades de los Animales Encefalitis Antimicrobianos Escherichia coli Animal Diseases Encephalitis Antimicrobials Cathelicidin are innate antimicrobial peptides with broad immunomodulatory functions; however, their role in regulating intestinal defenses is not well characterized. This study aimed to investigate the role of cathelicidin modulating expression of Toll-like receptors (TLRs) 4 and 9 in colonic epithelium in response to bacterial patterns. We demonstrated herein that intestinal epithelial cells, when primed by bacterial lipopolysaccharide (LPS), responded to cathelicidin by increased transcription and protein synthesis of TLR4. This cathelicidin-induced response required the interaction of LPS-TLR4 and activation of MAPK signalling pathways. However, cathelicidin blocked TLR9 responses induced by TLR9 ligand CpG oligodeoxynucleotide (CpG ODN) in these colonic epithelial cells. Modulations of TLRs triggered by cathelicidin in intestinal epithelium occurred mainly in the apical compartment of intestinal cells. Activation of TLR4 by ligands in combination with cathelicidin promoted CXCL8 chemokine secretion and epithelial antimicrobial defenses against Escherichia coli. We concluded that cathelicidin selectively modulated synthesis of TLR4 and 9 in intestinal epithelium, but only when cells were exposed to virulence factors, mostly from apical surfaces. Enhanced TLR4 expression promoted by cathelicidin in intestinal epithelium may be crucial for controlling enteric infectious diseases. EEA Balcarce Fil: Marin, Maia Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Holani, Ravi. University of Calgary. Faculty of Veterinary Medicine. Production Animal Health; Canadá Fil: Shah, Chaitanya B. University of Calgary. Bachelor of Health Sciences; Canadá Fil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Cobo, Eduardo R. University of Calgary. Faculty of Veterinary Medicine. Production Animal Health; Canadá 2018-03-13T17:16:25Z 2018-03-13T17:16:25Z 2017-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2018 https://www.sciencedirect.com/science/article/pii/S0161589017305102?via%3Dihub#! 0161-5890 https://doi.org/10.1016/j.molimm.2017.09.011 eng info:eu-repo/semantics/restrictedAccess application/pdf Molecular immunology 91 : 249-258. (November 2017) |
| spellingShingle | Enfermedades de los Animales Encefalitis Antimicrobianos Escherichia coli Animal Diseases Encephalitis Antimicrobials Marín, Maia Solange Holani, Ravi Shah, Chaitanya B. Odeon, Anselmo Carlos Cobo, Eduardo Rubén Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium |
| title | Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium |
| title_full | Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium |
| title_fullStr | Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium |
| title_full_unstemmed | Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium |
| title_short | Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium |
| title_sort | cathelicidin modulates synthesis of toll like receptors tlrs 4 and 9 in colonic epithelium |
| topic | Enfermedades de los Animales Encefalitis Antimicrobianos Escherichia coli Animal Diseases Encephalitis Antimicrobials |
| url | http://hdl.handle.net/20.500.12123/2018 https://www.sciencedirect.com/science/article/pii/S0161589017305102?via%3Dihub#! https://doi.org/10.1016/j.molimm.2017.09.011 |
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