Molecular identification and preliminary diversity analysis of Astylus atromaculatus Blanchard, 1843 (Coleoptera: Melyridae) based on mitochondrial COI sequences
Astylus atromaculatus Blanchard, 1843 (Coleoptera: Melyridae) is a pollinivorous beetle native to southern South America, which has invaded South Africa more than a century ago. Adults and/or larvae may occasionally damage flowers, seeds, and seedlings of various crops. Severe cattle intoxication ha...
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/20164 https://link.springer.com/article/10.1007/s12600-024-01195-x https://doi.org/10.1007/s12600-024-01195-x |
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| author | Arneodo Larochette, Joel Demian Decker Franco, Cecilia |
| author_browse | Arneodo Larochette, Joel Demian Decker Franco, Cecilia |
| author_facet | Arneodo Larochette, Joel Demian Decker Franco, Cecilia |
| author_sort | Arneodo Larochette, Joel Demian |
| collection | INTA Digital |
| description | Astylus atromaculatus Blanchard, 1843 (Coleoptera: Melyridae) is a pollinivorous beetle native to southern South America, which has invaded South Africa more than a century ago. Adults and/or larvae may occasionally damage flowers, seeds, and seedlings of various crops. Severe cattle intoxication has also been reported in Argentina, Uruguay, and South Africa following consumption of alfalfa and forage grasses infested with A. atromaculatus. Despite its economic impact, essential genetic information is lacking for this species. The present paper provides the first DNA barcode reference sequences for A. atromaculatus based on the standard 5’ fragment (658 bp) of the cytochrome c oxidase subunit I gene. The sequences obtained exhibited pairwise distances of ≤ 1.82% among them, and ~ 90% nucleotide identity with the homologous gene fragment in the morphologically similar Astylus variegatus Germar, 1824. The use of this molecular marker to explore the intraspecific variability of A. atromaculatus in central Argentina showed 21 different haplotypes, out of 32 individuals analyzed. A very high haplotype diversity (Hd = 0.962 ± 0.019) and a moderate nucleotide diversity (π = 0.00778 ± 0.00079) were recorded. The haplotype network displayed a diffuse structure due to the abundance of singletons and possible missing haplotypes, with the most common haplotype comprising only 15.6% of the specimens collected. Future research with increased sampling size and geographic coverage will allow for a better understanding of the population genetics of this pest, and consequently, for developing efficient management practices. |
| format | Artículo |
| id | INTA20164 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA201642024-11-07T14:59:10Z Molecular identification and preliminary diversity analysis of Astylus atromaculatus Blanchard, 1843 (Coleoptera: Melyridae) based on mitochondrial COI sequences Arneodo Larochette, Joel Demian Decker Franco, Cecilia Identificación Coleoptera Haplotipos ADN Identification Haplotypes DNA Astylus atromaculatus Astylus atromaculatus Blanchard, 1843 (Coleoptera: Melyridae) is a pollinivorous beetle native to southern South America, which has invaded South Africa more than a century ago. Adults and/or larvae may occasionally damage flowers, seeds, and seedlings of various crops. Severe cattle intoxication has also been reported in Argentina, Uruguay, and South Africa following consumption of alfalfa and forage grasses infested with A. atromaculatus. Despite its economic impact, essential genetic information is lacking for this species. The present paper provides the first DNA barcode reference sequences for A. atromaculatus based on the standard 5’ fragment (658 bp) of the cytochrome c oxidase subunit I gene. The sequences obtained exhibited pairwise distances of ≤ 1.82% among them, and ~ 90% nucleotide identity with the homologous gene fragment in the morphologically similar Astylus variegatus Germar, 1824. The use of this molecular marker to explore the intraspecific variability of A. atromaculatus in central Argentina showed 21 different haplotypes, out of 32 individuals analyzed. A very high haplotype diversity (Hd = 0.962 ± 0.019) and a moderate nucleotide diversity (π = 0.00778 ± 0.00079) were recorded. The haplotype network displayed a diffuse structure due to the abundance of singletons and possible missing haplotypes, with the most common haplotype comprising only 15.6% of the specimens collected. Future research with increased sampling size and geographic coverage will allow for a better understanding of the population genetics of this pest, and consequently, for developing efficient management practices. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMYZA); Argentina Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO) – INTA – CONICET; Argentina Fil: Arneodo Larochette, Joel Demian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola (IMYZA); Argentina Fil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO) – INTA – CONICET; Argentina 2024-11-07T14:47:09Z 2024-11-07T14:47:09Z 2024-09-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/20164 https://link.springer.com/article/10.1007/s12600-024-01195-x 0334-2123 (print) 1876-7184 (online) https://doi.org/10.1007/s12600-024-01195-x eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Phytoparasitica 52 : article number 76 (Septiembre 2024) |
| spellingShingle | Identificación Coleoptera Haplotipos ADN Identification Haplotypes DNA Astylus atromaculatus Arneodo Larochette, Joel Demian Decker Franco, Cecilia Molecular identification and preliminary diversity analysis of Astylus atromaculatus Blanchard, 1843 (Coleoptera: Melyridae) based on mitochondrial COI sequences |
| title | Molecular identification and preliminary diversity analysis of Astylus atromaculatus Blanchard, 1843 (Coleoptera: Melyridae) based on mitochondrial COI sequences |
| title_full | Molecular identification and preliminary diversity analysis of Astylus atromaculatus Blanchard, 1843 (Coleoptera: Melyridae) based on mitochondrial COI sequences |
| title_fullStr | Molecular identification and preliminary diversity analysis of Astylus atromaculatus Blanchard, 1843 (Coleoptera: Melyridae) based on mitochondrial COI sequences |
| title_full_unstemmed | Molecular identification and preliminary diversity analysis of Astylus atromaculatus Blanchard, 1843 (Coleoptera: Melyridae) based on mitochondrial COI sequences |
| title_short | Molecular identification and preliminary diversity analysis of Astylus atromaculatus Blanchard, 1843 (Coleoptera: Melyridae) based on mitochondrial COI sequences |
| title_sort | molecular identification and preliminary diversity analysis of astylus atromaculatus blanchard 1843 coleoptera melyridae based on mitochondrial coi sequences |
| topic | Identificación Coleoptera Haplotipos ADN Identification Haplotypes DNA Astylus atromaculatus |
| url | http://hdl.handle.net/20.500.12123/20164 https://link.springer.com/article/10.1007/s12600-024-01195-x https://doi.org/10.1007/s12600-024-01195-x |
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