Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV chall...
| Main Authors: | , , , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2018
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| Online Access: | http://hdl.handle.net/20.500.12123/2006 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185184 https://doi.org/10.1371/journal.pone.0185184 |
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| author | Soria, Ivana Quattrocchi, Valeria Langellotti, Cecilia Ana Gammella, Mariela Di Giacomo, Sebastián Garcia de la Torre, Beatriz Andreu, David Montoya, Maria Sobrino, Francisco Blanco, Esther Zamorano, Patricia Ines |
| author_browse | Andreu, David Blanco, Esther Di Giacomo, Sebastián Gammella, Mariela Garcia de la Torre, Beatriz Langellotti, Cecilia Ana Montoya, Maria Quattrocchi, Valeria Sobrino, Francisco Soria, Ivana Zamorano, Patricia Ines |
| author_facet | Soria, Ivana Quattrocchi, Valeria Langellotti, Cecilia Ana Gammella, Mariela Di Giacomo, Sebastián Garcia de la Torre, Beatriz Andreu, David Montoya, Maria Sobrino, Francisco Blanco, Esther Zamorano, Patricia Ines |
| author_sort | Soria, Ivana |
| collection | INTA Digital |
| description | Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [VP1(135–160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2006 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA20062018-03-09T15:57:09Z Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle Soria, Ivana Quattrocchi, Valeria Langellotti, Cecilia Ana Gammella, Mariela Di Giacomo, Sebastián Garcia de la Torre, Beatriz Andreu, David Montoya, Maria Sobrino, Francisco Blanco, Esther Zamorano, Patricia Ines Enfermedades de los Animales Virus de los Animales Ganado Bovino Fiebre Aftosa Péptidos Animal Diseases Animal Viruses Cattle Foot and Mouth Disease Peptides Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [VP1(135–160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential. Instituto de Virología Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Garcia de la Torre, Beatriz. Universitat Pompeu-Fabra. Departament de Ciencies Experimentals i de la Salut; España Fil: Andreu, David. Universitat Pompeu-Fabra. Departament de Ciencies Experimentals i de la Salut; España Fil: Montoya, María. Pirbright Institute; Gran Bretaña Fil: Sobrino, Francisco. Universidad Autónoma de Madrid. Centro de Biología Molecular “Severo Ochoa"; España Fil: Blanco, Esther. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Centro de Investigación en Sanidad Animal; España Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-03-09T15:54:33Z 2018-03-09T15:54:33Z 2017-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2006 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185184 1932-6203 https://doi.org/10.1371/journal.pone.0185184 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf PLoS ONE 12 (9) : e0185184 (2017) |
| spellingShingle | Enfermedades de los Animales Virus de los Animales Ganado Bovino Fiebre Aftosa Péptidos Animal Diseases Animal Viruses Cattle Foot and Mouth Disease Peptides Soria, Ivana Quattrocchi, Valeria Langellotti, Cecilia Ana Gammella, Mariela Di Giacomo, Sebastián Garcia de la Torre, Beatriz Andreu, David Montoya, Maria Sobrino, Francisco Blanco, Esther Zamorano, Patricia Ines Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle |
| title | Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle |
| title_full | Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle |
| title_fullStr | Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle |
| title_full_unstemmed | Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle |
| title_short | Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle |
| title_sort | dendrimeric peptides can confer protection against foot and mouth disease virus in cattle |
| topic | Enfermedades de los Animales Virus de los Animales Ganado Bovino Fiebre Aftosa Péptidos Animal Diseases Animal Viruses Cattle Foot and Mouth Disease Peptides |
| url | http://hdl.handle.net/20.500.12123/2006 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185184 https://doi.org/10.1371/journal.pone.0185184 |
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