Root-shoot ratio and SOD activity are associated with the sensitivity of common bean seedlings to NaCl salinization
The response of two common bean (Phaseolus vulgaris) varieties with different sensitivities to salinity (Montalbán: sensitive; I-193: moderately sensitive) was studied to determine some phytometric and physiological characteristics that could be associated with their differential sensitivities. Plan...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/20025 https://www.sciencedirect.com/science/article/abs/pii/S2452219824000016 https://doi.org/10.1016/j.rhisph.2024.100848 |
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| author | García, Marina García, Grisaly Parola, Rodrigo Raju Maddela, Naga Pérez-Almeida, Iris Garcés-Fiallos, Felipe R. |
| author_browse | Garcés-Fiallos, Felipe R. García, Grisaly García, Marina Parola, Rodrigo Pérez-Almeida, Iris Raju Maddela, Naga |
| author_facet | García, Marina García, Grisaly Parola, Rodrigo Raju Maddela, Naga Pérez-Almeida, Iris Garcés-Fiallos, Felipe R. |
| author_sort | García, Marina |
| collection | INTA Digital |
| description | The response of two common bean (Phaseolus vulgaris) varieties with different sensitivities to salinity (Montalbán: sensitive; I-193: moderately sensitive) was studied to determine some phytometric and physiological characteristics that could be associated with their differential sensitivities. Plants were sown in a greenhouse during the seedling stage and irrigated with a nutrient solution to which NaCl of 60 mol m−3 was added, while a control group was maintained in parallel. In Montalban, salinity caused a significant reduction in root biomass while shoot biomass and leaf area decreased with salt stress in both genotypes. The root/shoot ratio slightly increased in I.193 and decreased in Montalban salinized plants. Chlorophyll content increased with salinity in both genotypes. The presence of O2·- (superoxide radicle) in root apex decreased under the saline condition which did not occur in the case of H2O2. Superoxide dismutase (SOD) activity increased in roots and primary leaves under salt stress to a greater extent in I-193 than in Montalban. Four SOD isoenzymes were detected, one MnSOD, one FeSOD, and two Cu/ZnSOD, but their expression varied according to the variety. Catalase (CAT) activity increased only in the roots of stressed Montalban plants. These results suggest that root/shoot ratio and SOD enzyme activity are related to the lower salt sensitivity shown by I-193. |
| format | Artículo |
| id | INTA20025 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA200252024-10-31T14:03:26Z Root-shoot ratio and SOD activity are associated with the sensitivity of common bean seedlings to NaCl salinization García, Marina García, Grisaly Parola, Rodrigo Raju Maddela, Naga Pérez-Almeida, Iris Garcés-Fiallos, Felipe R. Common Beans Catalase Reactive Oxygen Species Salinity Superoxide Dismutase Fríjol (Phaseolus) Catalasa Especies de Oxígeno Reactivo Salinidad Superóxido Dismutasa The response of two common bean (Phaseolus vulgaris) varieties with different sensitivities to salinity (Montalbán: sensitive; I-193: moderately sensitive) was studied to determine some phytometric and physiological characteristics that could be associated with their differential sensitivities. Plants were sown in a greenhouse during the seedling stage and irrigated with a nutrient solution to which NaCl of 60 mol m−3 was added, while a control group was maintained in parallel. In Montalban, salinity caused a significant reduction in root biomass while shoot biomass and leaf area decreased with salt stress in both genotypes. The root/shoot ratio slightly increased in I.193 and decreased in Montalban salinized plants. Chlorophyll content increased with salinity in both genotypes. The presence of O2·- (superoxide radicle) in root apex decreased under the saline condition which did not occur in the case of H2O2. Superoxide dismutase (SOD) activity increased in roots and primary leaves under salt stress to a greater extent in I-193 than in Montalban. Four SOD isoenzymes were detected, one MnSOD, one FeSOD, and two Cu/ZnSOD, but their expression varied according to the variety. Catalase (CAT) activity increased only in the roots of stressed Montalban plants. These results suggest that root/shoot ratio and SOD enzyme activity are related to the lower salt sensitivity shown by I-193. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: García, Marina. Technical University of Manabí. Faculty of Agronomic Engineering. Department of Agricultural Sciences; Ecuador Fil: García, Marina. Central University of Venezuela. Faculty of Agronomy. Institute of Agricultural Botany; Venezuela Fil: García, Grisaly. Lisandro Alvarado Central Western University. Department of Biological Sciences. Dean of Agronomy; Venezuela Fil: Parola, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Parola, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Raju Maddela, Naga. Technical University of Manabí. Faculty of Health Sciences. Department of Biological Sciences; Ecuador Fil: Pérez-Almeida, Iris. Ecotec University. Research Center for Sustainable Development; Ecuador Fil: Garcés-Fiallos, Felipe R. Technical University of Manabí. Faculty of Agronomic Engineering. Laboratory of Phytopathology. Experimental Campus La Teodomira; Ecuador 2024-10-31T13:47:46Z 2024-10-31T13:47:46Z 2024-03-20 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/20025 https://www.sciencedirect.com/science/article/abs/pii/S2452219824000016 2452-2198 https://doi.org/10.1016/j.rhisph.2024.100848 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Rhizosphere 29 : 100848 (March 2024) |
| spellingShingle | Common Beans Catalase Reactive Oxygen Species Salinity Superoxide Dismutase Fríjol (Phaseolus) Catalasa Especies de Oxígeno Reactivo Salinidad Superóxido Dismutasa García, Marina García, Grisaly Parola, Rodrigo Raju Maddela, Naga Pérez-Almeida, Iris Garcés-Fiallos, Felipe R. Root-shoot ratio and SOD activity are associated with the sensitivity of common bean seedlings to NaCl salinization |
| title | Root-shoot ratio and SOD activity are associated with the sensitivity of common bean seedlings to NaCl salinization |
| title_full | Root-shoot ratio and SOD activity are associated with the sensitivity of common bean seedlings to NaCl salinization |
| title_fullStr | Root-shoot ratio and SOD activity are associated with the sensitivity of common bean seedlings to NaCl salinization |
| title_full_unstemmed | Root-shoot ratio and SOD activity are associated with the sensitivity of common bean seedlings to NaCl salinization |
| title_short | Root-shoot ratio and SOD activity are associated with the sensitivity of common bean seedlings to NaCl salinization |
| title_sort | root shoot ratio and sod activity are associated with the sensitivity of common bean seedlings to nacl salinization |
| topic | Common Beans Catalase Reactive Oxygen Species Salinity Superoxide Dismutase Fríjol (Phaseolus) Catalasa Especies de Oxígeno Reactivo Salinidad Superóxido Dismutasa |
| url | http://hdl.handle.net/20.500.12123/20025 https://www.sciencedirect.com/science/article/abs/pii/S2452219824000016 https://doi.org/10.1016/j.rhisph.2024.100848 |
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