Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
Closely related bovine alphaherpesviruses 1 (BoHV-1) and 5 (BoHV-5) co-circulate in certain countries, rendering cattle co-infection possible. This is a prerequisite for BoHV recombination. Here, we report the first identification of homologous recombination between field isolates of BoHV-1 and BoHV...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/2000 https://www.sciencedirect.com/science/article/pii/S0168170217302976?via%3Dihub https://doi.org/10.1016/j.virusres.2017.09.018 |
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| author | Maidana, Silvina Soledad Craig, Patricio Oliver Craig, María Isabel Ludwig, Louisa Mauroy, Axel Thiry, Etienne Romera, Sonia Alejandra |
| author_browse | Craig, María Isabel Craig, Patricio Oliver Ludwig, Louisa Maidana, Silvina Soledad Mauroy, Axel Romera, Sonia Alejandra Thiry, Etienne |
| author_facet | Maidana, Silvina Soledad Craig, Patricio Oliver Craig, María Isabel Ludwig, Louisa Mauroy, Axel Thiry, Etienne Romera, Sonia Alejandra |
| author_sort | Maidana, Silvina Soledad |
| collection | INTA Digital |
| description | Closely related bovine alphaherpesviruses 1 (BoHV-1) and 5 (BoHV-5) co-circulate in certain countries, rendering cattle co-infection possible. This is a prerequisite for BoHV recombination. Here, we report the first identification of homologous recombination between field isolates of BoHV-1 and BoHV-5, two alphaherpesviruses belonging to two distinct species with an average genomic similarity of 82.3%. Three isolates of BoHV-5, previously classified as subtype “BoHV-5b”, were phylogenetically studied and analyzed via eight PCR sequencing assays dispersed at regular intervals throughout the genome to discriminate between BoHV-1 andBoHV-5. In the phylogenetic analysis, differences of clustering were found in the UL27 gene which encodes the glycoprotein B (gB). We detected two recombination breakpoints in the open reading frame of the UL27 gene.
We compared the amino acid sequences of the gB of BoHV-1.1 and 1.2, BoHV-5a and recombinant formerly named BoHV-5b (chimeric gB) and subsequently performed molecular modeling. All structures were alike and, simultaneously, similar to the chimeric gB. Neutralizing antibodies against BoHV-1, BoHV-5 and recombinant viruses were analyzed via serum virus neutralization test using polyclonal sera and a monoclonal antibody against gB to demonstrate an absence of viral escape for both assays.
Our results show that homologous recombination between two related species of ruminant alphaherpesviruses can occur in natural field conditions. We found three recombinant field isolates, previously classified as BoHV-5b subtypes, between BoHV-1 and BoHV-5. |
| format | Artículo |
| id | INTA2000 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA20002018-06-21T17:31:46Z Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5 Maidana, Silvina Soledad Craig, Patricio Oliver Craig, María Isabel Ludwig, Louisa Mauroy, Axel Thiry, Etienne Romera, Sonia Alejandra Ganado Bovino Enfermedades de los Animales Herpesviridae Herpes Virus Bovino Cattle Animal Diseases Bovine Herpesvirus Herpesvirus Closely related bovine alphaherpesviruses 1 (BoHV-1) and 5 (BoHV-5) co-circulate in certain countries, rendering cattle co-infection possible. This is a prerequisite for BoHV recombination. Here, we report the first identification of homologous recombination between field isolates of BoHV-1 and BoHV-5, two alphaherpesviruses belonging to two distinct species with an average genomic similarity of 82.3%. Three isolates of BoHV-5, previously classified as subtype “BoHV-5b”, were phylogenetically studied and analyzed via eight PCR sequencing assays dispersed at regular intervals throughout the genome to discriminate between BoHV-1 andBoHV-5. In the phylogenetic analysis, differences of clustering were found in the UL27 gene which encodes the glycoprotein B (gB). We detected two recombination breakpoints in the open reading frame of the UL27 gene. We compared the amino acid sequences of the gB of BoHV-1.1 and 1.2, BoHV-5a and recombinant formerly named BoHV-5b (chimeric gB) and subsequently performed molecular modeling. All structures were alike and, simultaneously, similar to the chimeric gB. Neutralizing antibodies against BoHV-1, BoHV-5 and recombinant viruses were analyzed via serum virus neutralization test using polyclonal sera and a monoclonal antibody against gB to demonstrate an absence of viral escape for both assays. Our results show that homologous recombination between two related species of ruminant alphaherpesviruses can occur in natural field conditions. We found three recombinant field isolates, previously classified as BoHV-5b subtypes, between BoHV-1 and BoHV-5. Inst.de Virología Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Inmunogenética; Argentina Fil: Craig, Patricio Oliver. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Biológica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Ludwig, Louisa. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgica Fil: Mauroy, Axel. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgica Fil: Thiry, Etienne. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgica Fil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Inmunogenética; Argentina. Universidad del Salvador. Cátedra de Inmunología; Argentina 2018-03-09T11:25:58Z 2018-03-09T11:25:58Z 2017-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:ar-repo/semantics/artículo http://hdl.handle.net/20.500.12123/2000 https://www.sciencedirect.com/science/article/pii/S0168170217302976?via%3Dihub 0168-1702 https://doi.org/10.1016/j.virusres.2017.09.018 eng info:eu-repo/semantics/restrictedAccess application/pdf Virus research 242 : 122-130. (October 2017) |
| spellingShingle | Ganado Bovino Enfermedades de los Animales Herpesviridae Herpes Virus Bovino Cattle Animal Diseases Bovine Herpesvirus Herpesvirus Maidana, Silvina Soledad Craig, Patricio Oliver Craig, María Isabel Ludwig, Louisa Mauroy, Axel Thiry, Etienne Romera, Sonia Alejandra Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5 |
| title | Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5 |
| title_full | Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5 |
| title_fullStr | Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5 |
| title_full_unstemmed | Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5 |
| title_short | Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5 |
| title_sort | evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5 |
| topic | Ganado Bovino Enfermedades de los Animales Herpesviridae Herpes Virus Bovino Cattle Animal Diseases Bovine Herpesvirus Herpesvirus |
| url | http://hdl.handle.net/20.500.12123/2000 https://www.sciencedirect.com/science/article/pii/S0168170217302976?via%3Dihub https://doi.org/10.1016/j.virusres.2017.09.018 |
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