Effects of Clostridium perfringens iota toxin in the small intestine of mice
Iota toxin is a binary toxin solely produced by Clostridium perfringens type E strains, and is structurally related to CDT from C. difficile and CST from C. spiroforme. As type E causes hemorrhagic enteritis in cattle, it is usually assumed that associated diseases are mediated by iota toxin, althou...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1995 https://www.sciencedirect.com/science/article/pii/S1075996417301518?via%3Dihub https://doi.org/10.1016/j.anaerobe.2017.07.007 |
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| author | Redondo, Leandro Martin Redondo, Enzo Alejandro Dailoff, Gabriela Cecilia Leiva, Carlos Diaz Carrasco, Juan Maria Bruzzone, Octavio Augusto Cangelosi, Adriana Geoghegan, Patricia Fernandez Miyakawa, Mariano Enrique |
| author_browse | Bruzzone, Octavio Augusto Cangelosi, Adriana Dailoff, Gabriela Cecilia Diaz Carrasco, Juan Maria Fernandez Miyakawa, Mariano Enrique Geoghegan, Patricia Leiva, Carlos Redondo, Enzo Alejandro Redondo, Leandro Martin |
| author_facet | Redondo, Leandro Martin Redondo, Enzo Alejandro Dailoff, Gabriela Cecilia Leiva, Carlos Diaz Carrasco, Juan Maria Bruzzone, Octavio Augusto Cangelosi, Adriana Geoghegan, Patricia Fernandez Miyakawa, Mariano Enrique |
| author_sort | Redondo, Leandro Martin |
| collection | INTA Digital |
| description | Iota toxin is a binary toxin solely produced by Clostridium perfringens type E strains, and is structurally related to CDT from C. difficile and CST from C. spiroforme. As type E causes hemorrhagic enteritis in cattle, it is usually assumed that associated diseases are mediated by iota toxin, although evidence in this regar has not been provided. In the present report, iota toxin intestinal effects were evaluated in vivo using a mouse model. Histological damage was observed in ileal loops treated with purified iota toxin after 4 h of incubation. Luminal iota toxin induced fluid accumulation in the small intestine in a dose dependent
manner, as determined by the enteropooling and the intestinal loop assays. None of these changes were observed in the large intestine. These results suggest that C. perfringens iota toxin alters intestinal permeability, predominantly by inducing necrosis and degenerative changes in the mucosal epithelium of the small intestine, as well as changes in intestinal motility. The obtained results suggest a central role for iota toxin in the pathogenesis of C. perfringens type E hemorrhagic enteritis, and contribute to remark the importance of clostridial binary toxins in digestive diseases. |
| format | Artículo |
| id | INTA1995 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA19952019-03-21T18:40:35Z Effects of Clostridium perfringens iota toxin in the small intestine of mice Redondo, Leandro Martin Redondo, Enzo Alejandro Dailoff, Gabriela Cecilia Leiva, Carlos Diaz Carrasco, Juan Maria Bruzzone, Octavio Augusto Cangelosi, Adriana Geoghegan, Patricia Fernandez Miyakawa, Mariano Enrique Clostridium Perfringens Toxinas Ratón Enfermedades de los Animales Toxins Mice Animal Diseases Iota toxin is a binary toxin solely produced by Clostridium perfringens type E strains, and is structurally related to CDT from C. difficile and CST from C. spiroforme. As type E causes hemorrhagic enteritis in cattle, it is usually assumed that associated diseases are mediated by iota toxin, although evidence in this regar has not been provided. In the present report, iota toxin intestinal effects were evaluated in vivo using a mouse model. Histological damage was observed in ileal loops treated with purified iota toxin after 4 h of incubation. Luminal iota toxin induced fluid accumulation in the small intestine in a dose dependent manner, as determined by the enteropooling and the intestinal loop assays. None of these changes were observed in the large intestine. These results suggest that C. perfringens iota toxin alters intestinal permeability, predominantly by inducing necrosis and degenerative changes in the mucosal epithelium of the small intestine, as well as changes in intestinal motility. The obtained results suggest a central role for iota toxin in the pathogenesis of C. perfringens type E hemorrhagic enteritis, and contribute to remark the importance of clostridial binary toxins in digestive diseases. Inst. de Patobiología Fil: Redondo, Leandro Martín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Redondo, Enzo Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dailoff, Gabriela Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Leiva, Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Diaz Carrasco, Juan Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bruzzone, Octavio Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cangelosi, Adriana. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Geoghegan, Patricia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-03-08T14:31:03Z 2018-03-08T14:31:03Z 2017-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1995 https://www.sciencedirect.com/science/article/pii/S1075996417301518?via%3Dihub 1075-9964 https://doi.org/10.1016/j.anaerobe.2017.07.007 eng info:eu-repo/semantics/restrictedAccess application/pdf Anaerobe 48 : 83-88. (December 2017) |
| spellingShingle | Clostridium Perfringens Toxinas Ratón Enfermedades de los Animales Toxins Mice Animal Diseases Redondo, Leandro Martin Redondo, Enzo Alejandro Dailoff, Gabriela Cecilia Leiva, Carlos Diaz Carrasco, Juan Maria Bruzzone, Octavio Augusto Cangelosi, Adriana Geoghegan, Patricia Fernandez Miyakawa, Mariano Enrique Effects of Clostridium perfringens iota toxin in the small intestine of mice |
| title | Effects of Clostridium perfringens iota toxin in the small intestine of mice |
| title_full | Effects of Clostridium perfringens iota toxin in the small intestine of mice |
| title_fullStr | Effects of Clostridium perfringens iota toxin in the small intestine of mice |
| title_full_unstemmed | Effects of Clostridium perfringens iota toxin in the small intestine of mice |
| title_short | Effects of Clostridium perfringens iota toxin in the small intestine of mice |
| title_sort | effects of clostridium perfringens iota toxin in the small intestine of mice |
| topic | Clostridium Perfringens Toxinas Ratón Enfermedades de los Animales Toxins Mice Animal Diseases |
| url | http://hdl.handle.net/20.500.12123/1995 https://www.sciencedirect.com/science/article/pii/S1075996417301518?via%3Dihub https://doi.org/10.1016/j.anaerobe.2017.07.007 |
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