Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease
Peach Leaf Curl Disease, caused by the fungus Taphrina deformans, is characterized by reddish hypertrophic and hyperplasic leaf areas. To comprehend the biochemical imbalances caused by the disease an integrated approach including metabolomics, lipidomics, proteomics and complementary biochemical te...
| Main Authors: | , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Wiley
2024
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/19816 https://onlinelibrary.wiley.com/doi/10.1111/pce.15210 https://doi.org/10.1111/pce.15210 |
| _version_ | 1855037936663265280 |
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| author | Novello, María Angelina Bustamante, Claudia Anabel Svetaz, Laura Goldy, Camila Valentini, Gabriel Hugo Drincovich, María Fabiana Brotman, Yadiv Fernie, Alisdair R. Lara, María Valeria |
| author_browse | Brotman, Yadiv Bustamante, Claudia Anabel Drincovich, María Fabiana Fernie, Alisdair R. Goldy, Camila Lara, María Valeria Novello, María Angelina Svetaz, Laura Valentini, Gabriel Hugo |
| author_facet | Novello, María Angelina Bustamante, Claudia Anabel Svetaz, Laura Goldy, Camila Valentini, Gabriel Hugo Drincovich, María Fabiana Brotman, Yadiv Fernie, Alisdair R. Lara, María Valeria |
| author_sort | Novello, María Angelina |
| collection | INTA Digital |
| description | Peach Leaf Curl Disease, caused by the fungus Taphrina deformans, is characterized by reddish hypertrophic and hyperplasic leaf areas. To comprehend the biochemical imbalances caused by the disease an integrated approach including metabolomics, lipidomics, proteomics and complementary biochemical techniques was undertaken. Symptomatic and asymptomatic areas were dissected from leaves with increasing extension of the disease. A differential metabolic behaviour was identified in symptomatic areas with respect to either asymptomatic areas or healthy leaves. Symptomatic areas showed an altered chloroplastic functioning and composition which differs from the typical senescence process and includes decrease in the photosynthetic machinery, alteration in plastidic lipids, and decreased starch, carotenoid and chlorophyll biosynthesis. In symptomatic areas, alteration in redox-homeostasis proteins and in triacylglycerols content, peroxidation and double bond index were observed. Proteomic data revealed induction of host enzymes involved in auxin and jasmonate biosynthesis together with up-regulation of phenylpropanoid and mevalonate pathways and down-regulation of the plastidic methylerythritol phosphate route. Amino acid pools were affected, with up-regulation of proteins involved in asparagine synthesis. Overall, we conclude that curled areas exhibited a metabolic shift towards functioning as a sink tissue importing sugars and producing energy through fermentation and respiration and reductive power via the pentose phosphate route. |
| format | info:ar-repo/semantics/artículo |
| id | INTA19816 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA198162024-10-22T12:33:29Z Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease Novello, María Angelina Bustamante, Claudia Anabel Svetaz, Laura Goldy, Camila Valentini, Gabriel Hugo Drincovich, María Fabiana Brotman, Yadiv Fernie, Alisdair R. Lara, María Valeria Durazno Prunus persica Genética Genómica Asparagina Glicosidos Cianogénicos Metaboloma Proteómica Taphrina deformans Peaches Genetics Genomics Asparagine Cyanogenic Glucosides Metabolome Proteomics Peach Leaf Curl Disease, caused by the fungus Taphrina deformans, is characterized by reddish hypertrophic and hyperplasic leaf areas. To comprehend the biochemical imbalances caused by the disease an integrated approach including metabolomics, lipidomics, proteomics and complementary biochemical techniques was undertaken. Symptomatic and asymptomatic areas were dissected from leaves with increasing extension of the disease. A differential metabolic behaviour was identified in symptomatic areas with respect to either asymptomatic areas or healthy leaves. Symptomatic areas showed an altered chloroplastic functioning and composition which differs from the typical senescence process and includes decrease in the photosynthetic machinery, alteration in plastidic lipids, and decreased starch, carotenoid and chlorophyll biosynthesis. In symptomatic areas, alteration in redox-homeostasis proteins and in triacylglycerols content, peroxidation and double bond index were observed. Proteomic data revealed induction of host enzymes involved in auxin and jasmonate biosynthesis together with up-regulation of phenylpropanoid and mevalonate pathways and down-regulation of the plastidic methylerythritol phosphate route. Amino acid pools were affected, with up-regulation of proteins involved in asparagine synthesis. Overall, we conclude that curled areas exhibited a metabolic shift towards functioning as a sink tissue importing sugars and producing energy through fermentation and respiration and reductive power via the pentose phosphate route. EEA San Pedro, INTA Fil: Novello, María Angelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Bustamante, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Svetaz, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Goldy, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Valentini, Gabriel Hugo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Brotman, Yariv. Tel Aviv University. The George S. Wise Faculty of Life Sciences. School of Plant Sciences and Food Security; Israel Fil: Fernie, Alisdair R. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania Fil: Lara, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina 2024-10-22T12:26:58Z 2024-10-22T12:26:58Z 2024-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/19816 https://onlinelibrary.wiley.com/doi/10.1111/pce.15210 Novello, M.A., Bustamante, C.A., Svetaz, L.A., Goldy, C., Valentini, G.H., Drincovich, M.F., Brotman, Y., Fernie, A.R. and Lara, M.V. (2024), Integrated Metabolomic, Lipidomic and Proteomic Analysis Define the Metabolic Changes Occurring in Curled Areas in Leaves With Leaf Peach Curl Disease. Plant, Cell & Environment. https://doi.org/10.1111/pce.15210 1365-3040 0140-7791 https://doi.org/10.1111/pce.15210 eng info:eu-repograntAgreement/INTA/2019-PE-E6-I125-001, Mejoramiento genético, caracterización y uso de variabilidad con aplicación de herramientas biotecnológicas en cultivos frutales info:eu-repograntAgreement/INTA/2023-PE-L01-I105, Generación de conocimientos, tecnologías e innovaciones para una fruticultura sostenible adaptadas al riesgo ambiental y a la mecanización info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Plant, Cell & Environment : e15210 (October 2024) |
| spellingShingle | Durazno Prunus persica Genética Genómica Asparagina Glicosidos Cianogénicos Metaboloma Proteómica Taphrina deformans Peaches Genetics Genomics Asparagine Cyanogenic Glucosides Metabolome Proteomics Novello, María Angelina Bustamante, Claudia Anabel Svetaz, Laura Goldy, Camila Valentini, Gabriel Hugo Drincovich, María Fabiana Brotman, Yadiv Fernie, Alisdair R. Lara, María Valeria Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease |
| title | Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease |
| title_full | Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease |
| title_fullStr | Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease |
| title_full_unstemmed | Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease |
| title_short | Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease |
| title_sort | integrated metabolomic lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease |
| topic | Durazno Prunus persica Genética Genómica Asparagina Glicosidos Cianogénicos Metaboloma Proteómica Taphrina deformans Peaches Genetics Genomics Asparagine Cyanogenic Glucosides Metabolome Proteomics |
| url | http://hdl.handle.net/20.500.12123/19816 https://onlinelibrary.wiley.com/doi/10.1111/pce.15210 https://doi.org/10.1111/pce.15210 |
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