Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from Argentina
Hygienic behavior is an economically beneficial, heritable trait, which has evolved to limit the impact of honeybee pathogens. Selecting and breeding colonies with high levels of hygienic behavior has become a feasible and environmentally friendly strategy to control brood diseases in honeybee colon...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1977 https://link.springer.com/article/10.1007/s00040-017-0567-6 https://doi.org/10.1007/s00040-017-0567-6 |
| _version_ | 1855034884171497472 |
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| author | Scannapieco, Alejandra Carla Mannino, Constanza Soto, Gabriela Cinthia Palacio, María Alejandra Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz |
| author_browse | Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz Mannino, Constanza Palacio, María Alejandra Scannapieco, Alejandra Carla Soto, Gabriela Cinthia |
| author_facet | Scannapieco, Alejandra Carla Mannino, Constanza Soto, Gabriela Cinthia Palacio, María Alejandra Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz |
| author_sort | Scannapieco, Alejandra Carla |
| collection | INTA Digital |
| description | Hygienic behavior is an economically beneficial, heritable trait, which has evolved to limit the impact of honeybee pathogens. Selecting and breeding colonies with high levels of hygienic behavior has become a feasible and environmentally friendly strategy to control brood diseases in honeybee colonies worldwide. The identification of genes involved in the expression of this character may not only unravel molecular and biochemical pathways underlying hygienic behavior, but also serve as a practical approach to select disease resistance biomarkers useful for honeybee breeding programs. In the present work, we evaluated, at genetic level, Apis mellifera stocks selected for hygienic behavior, widely used for commercial apiculture in Argentina. We analyzed the expression profiles of five genes previously identified as candidates associated with hygienic behavior both in QTL and global gene expression studies in honeybees, more precisely, involved in perception and processing of olfactory information. We validated the differential expression of these genes as potentially responsible for behavioral differences in our selected stocks. Our results indicate that four of them (octopamine receptor, smell-impaired, odorant-binding protein 3, and odorant-binding protein 4) were differentially expressed between hygienic and non-hygienic bees within our highly hygienic colonies. The present findings improve our understanding of the molecular mechanisms underlying the differentiation of middle-age worker bees in their genetic propensity to perform hygienic behavior. This progress towards the genetic characterization of highly hygienic colonies that are commercially used in Argentine apiculture lays the groundwork for future development of targets for marker-assisted selection of disease-resistant honeybee stocks. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1977 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA19772019-03-21T18:37:42Z Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from Argentina Scannapieco, Alejandra Carla Mannino, Constanza Soto, Gabriela Cinthia Palacio, María Alejandra Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz Abeja Melífera Apis Mellifera Comportamiento Behaviour Genética Honey Bees Genetics Hygienic behavior is an economically beneficial, heritable trait, which has evolved to limit the impact of honeybee pathogens. Selecting and breeding colonies with high levels of hygienic behavior has become a feasible and environmentally friendly strategy to control brood diseases in honeybee colonies worldwide. The identification of genes involved in the expression of this character may not only unravel molecular and biochemical pathways underlying hygienic behavior, but also serve as a practical approach to select disease resistance biomarkers useful for honeybee breeding programs. In the present work, we evaluated, at genetic level, Apis mellifera stocks selected for hygienic behavior, widely used for commercial apiculture in Argentina. We analyzed the expression profiles of five genes previously identified as candidates associated with hygienic behavior both in QTL and global gene expression studies in honeybees, more precisely, involved in perception and processing of olfactory information. We validated the differential expression of these genes as potentially responsible for behavioral differences in our selected stocks. Our results indicate that four of them (octopamine receptor, smell-impaired, odorant-binding protein 3, and odorant-binding protein 4) were differentially expressed between hygienic and non-hygienic bees within our highly hygienic colonies. The present findings improve our understanding of the molecular mechanisms underlying the differentiation of middle-age worker bees in their genetic propensity to perform hygienic behavior. This progress towards the genetic characterization of highly hygienic colonies that are commercially used in Argentine apiculture lays the groundwork for future development of targets for marker-assisted selection of disease-resistant honeybee stocks. Inst. de Genética "Ewald A. Favret"- IGEAF Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mannino, Constanza. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Palacio, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina 2018-03-07T13:26:44Z 2018-03-07T13:26:44Z 2017-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1977 https://link.springer.com/article/10.1007/s00040-017-0567-6 0020-1812 (Print) 1420-9098 (Online) https://doi.org/10.1007/s00040-017-0567-6 eng info:eu-repo/semantics/restrictedAccess application/pdf Argentina (nation) Insectes sociaux 64 (4) : 485–494. (November 2017) |
| spellingShingle | Abeja Melífera Apis Mellifera Comportamiento Behaviour Genética Honey Bees Genetics Scannapieco, Alejandra Carla Mannino, Constanza Soto, Gabriela Cinthia Palacio, María Alejandra Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from Argentina |
| title | Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from Argentina |
| title_full | Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from Argentina |
| title_fullStr | Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from Argentina |
| title_full_unstemmed | Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from Argentina |
| title_short | Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from Argentina |
| title_sort | expression analysis of genes putatively associated with hygienic behavior in selected stocks of apis mellifera l from argentina |
| topic | Abeja Melífera Apis Mellifera Comportamiento Behaviour Genética Honey Bees Genetics |
| url | http://hdl.handle.net/20.500.12123/1977 https://link.springer.com/article/10.1007/s00040-017-0567-6 https://doi.org/10.1007/s00040-017-0567-6 |
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