Stable symbiotic nitrogen fixation under water-deficit field conditions by a stress-tolerant alfalfa microsymbiont and its complete genome sequence : Short communication

We here characterized the stress-tolerant alfalfa microsymbiont Sinorhizobium meliloti B401. B401-treated plants showed high nitrogen fixation rates under humid and semiarid environments. The production of glycine betaine in isolated bacteroids positively correlated with low precipitation levels, su...

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Main Authors: Jozefkowicz, Cintia, Brambilla, Silvina Maricel, Frare, Romina Alejandra, Stritzler, Margarita, Piccinetti, Carlos Fabian, Puente, Mariana Laura, Berini, Carolina Andrea, Reyes Perez, Pedro Jose, Soto, Gabriela Cinthia, Ayub, Nicolás Daniel
Format: info:eu-repo/semantics/article
Language:Inglés
Published: 2018
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/1974
https://www.sciencedirect.com/science/article/pii/S0168165617317029
https://doi.org/10.1016/j.jbiotec.2017.10.007
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author Jozefkowicz, Cintia
Brambilla, Silvina Maricel
Frare, Romina Alejandra
Stritzler, Margarita
Piccinetti, Carlos Fabian
Puente, Mariana Laura
Berini, Carolina Andrea
Reyes Perez, Pedro Jose
Soto, Gabriela Cinthia
Ayub, Nicolás Daniel
author_browse Ayub, Nicolás Daniel
Berini, Carolina Andrea
Brambilla, Silvina Maricel
Frare, Romina Alejandra
Jozefkowicz, Cintia
Piccinetti, Carlos Fabian
Puente, Mariana Laura
Reyes Perez, Pedro Jose
Soto, Gabriela Cinthia
Stritzler, Margarita
author_facet Jozefkowicz, Cintia
Brambilla, Silvina Maricel
Frare, Romina Alejandra
Stritzler, Margarita
Piccinetti, Carlos Fabian
Puente, Mariana Laura
Berini, Carolina Andrea
Reyes Perez, Pedro Jose
Soto, Gabriela Cinthia
Ayub, Nicolás Daniel
author_sort Jozefkowicz, Cintia
collection INTA Digital
description We here characterized the stress-tolerant alfalfa microsymbiont Sinorhizobium meliloti B401. B401-treated plants showed high nitrogen fixation rates under humid and semiarid environments. The production of glycine betaine in isolated bacteroids positively correlated with low precipitation levels, suggesting that this compound acts as a critical osmoprotectant under field conditions. Genome analysis revealed that strain B401 contains alternative pathways for the biosynthesis and uptake of glycine betaine and its precursors. Such genomic information will offer substantial insight into the environmental physiology of this biotechnologically valuable nitrogen-fixing bacterium.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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spelling INTA19742018-06-21T12:29:21Z Stable symbiotic nitrogen fixation under water-deficit field conditions by a stress-tolerant alfalfa microsymbiont and its complete genome sequence : Short communication Jozefkowicz, Cintia Brambilla, Silvina Maricel Frare, Romina Alejandra Stritzler, Margarita Piccinetti, Carlos Fabian Puente, Mariana Laura Berini, Carolina Andrea Reyes Perez, Pedro Jose Soto, Gabriela Cinthia Ayub, Nicolás Daniel Medicago Sativa Fijación del Nitrógeno Déficit de Humedad en el Suelo Simbiontico Secuencia Nucleotídica Nitrogen Fixation Soil Water Deficit Symbionts Nucleotide Sequence Alfalfa Secuencia Genómica We here characterized the stress-tolerant alfalfa microsymbiont Sinorhizobium meliloti B401. B401-treated plants showed high nitrogen fixation rates under humid and semiarid environments. The production of glycine betaine in isolated bacteroids positively correlated with low precipitation levels, suggesting that this compound acts as a critical osmoprotectant under field conditions. Genome analysis revealed that strain B401 contains alternative pathways for the biosynthesis and uptake of glycine betaine and its precursors. Such genomic information will offer substantial insight into the environmental physiology of this biotechnologically valuable nitrogen-fixing bacterium. Inst. de Microbiología y Zoología Agrícola IMyZA Fil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Piccinetti, Carlos Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Berini, Carolina Andrea. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Reyes Perez, Pedro Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-03-07T12:02:54Z 2018-03-07T12:02:54Z 2017-12 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1974 https://www.sciencedirect.com/science/article/pii/S0168165617317029 0168-1656 https://doi.org/10.1016/j.jbiotec.2017.10.007 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of biotechnology 263 : 52-54. (December 2017)
spellingShingle Medicago Sativa
Fijación del Nitrógeno
Déficit de Humedad en el Suelo
Simbiontico
Secuencia Nucleotídica
Nitrogen Fixation
Soil Water Deficit
Symbionts
Nucleotide Sequence
Alfalfa
Secuencia Genómica
Jozefkowicz, Cintia
Brambilla, Silvina Maricel
Frare, Romina Alejandra
Stritzler, Margarita
Piccinetti, Carlos Fabian
Puente, Mariana Laura
Berini, Carolina Andrea
Reyes Perez, Pedro Jose
Soto, Gabriela Cinthia
Ayub, Nicolás Daniel
Stable symbiotic nitrogen fixation under water-deficit field conditions by a stress-tolerant alfalfa microsymbiont and its complete genome sequence : Short communication
title Stable symbiotic nitrogen fixation under water-deficit field conditions by a stress-tolerant alfalfa microsymbiont and its complete genome sequence : Short communication
title_full Stable symbiotic nitrogen fixation under water-deficit field conditions by a stress-tolerant alfalfa microsymbiont and its complete genome sequence : Short communication
title_fullStr Stable symbiotic nitrogen fixation under water-deficit field conditions by a stress-tolerant alfalfa microsymbiont and its complete genome sequence : Short communication
title_full_unstemmed Stable symbiotic nitrogen fixation under water-deficit field conditions by a stress-tolerant alfalfa microsymbiont and its complete genome sequence : Short communication
title_short Stable symbiotic nitrogen fixation under water-deficit field conditions by a stress-tolerant alfalfa microsymbiont and its complete genome sequence : Short communication
title_sort stable symbiotic nitrogen fixation under water deficit field conditions by a stress tolerant alfalfa microsymbiont and its complete genome sequence short communication
topic Medicago Sativa
Fijación del Nitrógeno
Déficit de Humedad en el Suelo
Simbiontico
Secuencia Nucleotídica
Nitrogen Fixation
Soil Water Deficit
Symbionts
Nucleotide Sequence
Alfalfa
Secuencia Genómica
url http://hdl.handle.net/20.500.12123/1974
https://www.sciencedirect.com/science/article/pii/S0168165617317029
https://doi.org/10.1016/j.jbiotec.2017.10.007
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