In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolates
Background: Latent tuberculosis has been associated with the persistence of dormant Mycobacterium tuberculosis in the organism of infected individuals, who are reservoirs of the bacilli and the source for spreading the disease in the community. New active anti-TB drugs exerting their metabolic actio...
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| Format: | Artículo |
| Language: | Inglés |
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2018
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| Online Access: | http://hdl.handle.net/20.500.12123/1961 http://www.elsevier.es/en-revista-revista-argentina-microbiologia-372-resumen-in-vitro-anti-tuberculosis-activity-azole-S0325754117300895 http://dx.doi.org/10.1016/j.ram.2017.02.008 |
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| author | Imperiale, Belen Rocio Cataldi, Angel Adrian Morcillo, Nora |
| author_browse | Cataldi, Angel Adrian Imperiale, Belen Rocio Morcillo, Nora |
| author_facet | Imperiale, Belen Rocio Cataldi, Angel Adrian Morcillo, Nora |
| author_sort | Imperiale, Belen Rocio |
| collection | INTA Digital |
| description | Background: Latent tuberculosis has been associated with the persistence of dormant Mycobacterium tuberculosis in the organism of infected individuals, who are reservoirs of the bacilli and the source for spreading the disease in the community. New active anti-TB drugs exerting their metabolic action at different stages and on latent/dormant bacilli are urgently required to avoid endogenous reactivations and to be part of treatments of multi- and extensively-drug resistant tuberculosis (M/XDR-TB). It was previously reported that azole drugs are active against M. tuberculosis. For that reason, the aims of this study were to determine the in vitro activity of azole drugs, imidazole (clotrimazole, CLO and econazole, ECO) and nitroimidazole (metronidazole, MZ and ipronidazole, IPZ), against a collection of MDR M. tuberculosis clinical isolates; and to analyze their potential use in both the LTB and the active forms of M/XDR-TB treatments.
Methods: A total of 55 MDR M. tuberculosis isolates and H37Rv were included. MZ and IPZ activity against M. tuberculosis isolates were tested using anaerobic culture conditions.
The activity of ECO and CLO was measured by the minimal inhibitory concentration (MIC) using a microdilution colorimetric method. |
| format | Artículo |
| id | INTA1961 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA19612018-03-05T16:35:47Z In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolates Imperiale, Belen Rocio Cataldi, Angel Adrian Morcillo, Nora Mycobacterium Resistencia a Medicamentos Azoles Mycobacterium Tuberculosis Imidazoles Experimentación in Vitro Drug Resistance In Vitro Experimentation Background: Latent tuberculosis has been associated with the persistence of dormant Mycobacterium tuberculosis in the organism of infected individuals, who are reservoirs of the bacilli and the source for spreading the disease in the community. New active anti-TB drugs exerting their metabolic action at different stages and on latent/dormant bacilli are urgently required to avoid endogenous reactivations and to be part of treatments of multi- and extensively-drug resistant tuberculosis (M/XDR-TB). It was previously reported that azole drugs are active against M. tuberculosis. For that reason, the aims of this study were to determine the in vitro activity of azole drugs, imidazole (clotrimazole, CLO and econazole, ECO) and nitroimidazole (metronidazole, MZ and ipronidazole, IPZ), against a collection of MDR M. tuberculosis clinical isolates; and to analyze their potential use in both the LTB and the active forms of M/XDR-TB treatments. Methods: A total of 55 MDR M. tuberculosis isolates and H37Rv were included. MZ and IPZ activity against M. tuberculosis isolates were tested using anaerobic culture conditions. The activity of ECO and CLO was measured by the minimal inhibitory concentration (MIC) using a microdilution colorimetric method. Inst. de Biotecnología Fil: Imperiale, Belen Rocio. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Morcillo, Nora. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina 2018-03-05T15:41:50Z 2018-03-05T15:41:50Z 2017-12 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1961 http://www.elsevier.es/en-revista-revista-argentina-microbiologia-372-resumen-in-vitro-anti-tuberculosis-activity-azole-S0325754117300895 http://dx.doi.org/10.1016/j.ram.2017.02.008 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Revista Argentina de Microbiología 49 (4) : 332-338 (October - December 2017) |
| spellingShingle | Mycobacterium Resistencia a Medicamentos Azoles Mycobacterium Tuberculosis Imidazoles Experimentación in Vitro Drug Resistance In Vitro Experimentation Imperiale, Belen Rocio Cataldi, Angel Adrian Morcillo, Nora In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolates |
| title | In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolates |
| title_full | In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolates |
| title_fullStr | In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolates |
| title_full_unstemmed | In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolates |
| title_short | In vitro anti-tuberculosis activity of azole drugs against Mycobacterium tuberculosis clinical isolates |
| title_sort | in vitro anti tuberculosis activity of azole drugs against mycobacterium tuberculosis clinical isolates |
| topic | Mycobacterium Resistencia a Medicamentos Azoles Mycobacterium Tuberculosis Imidazoles Experimentación in Vitro Drug Resistance In Vitro Experimentation |
| url | http://hdl.handle.net/20.500.12123/1961 http://www.elsevier.es/en-revista-revista-argentina-microbiologia-372-resumen-in-vitro-anti-tuberculosis-activity-azole-S0325754117300895 http://dx.doi.org/10.1016/j.ram.2017.02.008 |
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