Pampas fox (Lycalopex gymnocercus) new intermediate host of Sarcocystis svanai (Apicomplexa: Sarcocystidae)
Several Sarcocystis spp. have carnivores as definitive host and sarcocysts are common in muscles of herbivores (intermediate host). However, sarcocysts have been found in muscles of wild and domestic carnivores suggesting they are intermediate host for some Sarcocystis spp. Here, we report mature sa...
| Main Authors: | , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2024
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/19605 https://www.sciencedirect.com/science/article/pii/S1383576917300028 https://doi.org/10.1016/j.parint.2017.01.021 |
| _version_ | 1855486407783481344 |
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| author | Scioscia, Nathalia Olmos, Leandro Hipolito Gorosabel, Antonella Bernad, Lucía Pedrana, Julieta Hecker, Yanina Gual, Ignacio Gos, Maria Laura Denegri, Guillermo Moore, Prando Dadin Moré, Gastón Andrés |
| author_browse | Bernad, Lucía Denegri, Guillermo Gorosabel, Antonella Gos, Maria Laura Gual, Ignacio Hecker, Yanina Moore, Prando Dadin Moré, Gastón Andrés Olmos, Leandro Hipolito Pedrana, Julieta Scioscia, Nathalia |
| author_facet | Scioscia, Nathalia Olmos, Leandro Hipolito Gorosabel, Antonella Bernad, Lucía Pedrana, Julieta Hecker, Yanina Gual, Ignacio Gos, Maria Laura Denegri, Guillermo Moore, Prando Dadin Moré, Gastón Andrés |
| author_sort | Scioscia, Nathalia |
| collection | INTA Digital |
| description | Several Sarcocystis spp. have carnivores as definitive host and sarcocysts are common in muscles of herbivores (intermediate host). However, sarcocysts have been found in muscles of wild and domestic carnivores suggesting they are intermediate host for some Sarcocystis spp. Here, we report mature sarcocysts in the muscles of Pampas fox (Lycalopex gymnocercus). A total of 36 free-living foxes were analyzed. Different skeletal muscles were assessed by microscopic and molecular methods. Cysts and/or DNA of Sarcocystis sp. were detected in 61.1% (22/36) foxes. Histopathology revealed the presence of sarcocysts in 52.8% (19/36) foxes. The tongue and masseter were the muscles more frequently infected. Of all the samples processed by homogenization of pooled muscles of each animal, 45.4% (10/22) evidenced muscle cysts and 68.2% (15/22) resulted positives by PCR. Individual cysts obtained from the ten positive samples in direct microscopic examination were all positive by PCR. Five amplicons from individual cysts from different samples were selected for sequencing together with four PCR products obtained from the pooled muscles. All nine sequences shared a high identity among them (99.8–100%) and showed the highest identity by BLAST (99%) with a S. svanai sequence (KM362428) from a North American dog. By transmission electron microscopy, the sarcocyst wall was thin (< 1 μm), had minute undulations, with tiny evaginations and without evident villar protrusions. The cyst wall type is referred as “type 1”. Sarcocystis svanai infects L. gymnocercus with a high prevalence and the presence of mature sarcocysts suggests the role of the Pampa. |
| format | Artículo |
| id | INTA19605 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA196052024-09-30T11:55:04Z Pampas fox (Lycalopex gymnocercus) new intermediate host of Sarcocystis svanai (Apicomplexa: Sarcocystidae) Scioscia, Nathalia Olmos, Leandro Hipolito Gorosabel, Antonella Bernad, Lucía Pedrana, Julieta Hecker, Yanina Gual, Ignacio Gos, Maria Laura Denegri, Guillermo Moore, Prando Dadin Moré, Gastón Andrés Histología Zorro Músculo Sarcocystis Histology Foxes Muscles Intermediate Hosts Huéspedes Intermediarios Lycalopex gymnocercus Sarcocystis svanai Several Sarcocystis spp. have carnivores as definitive host and sarcocysts are common in muscles of herbivores (intermediate host). However, sarcocysts have been found in muscles of wild and domestic carnivores suggesting they are intermediate host for some Sarcocystis spp. Here, we report mature sarcocysts in the muscles of Pampas fox (Lycalopex gymnocercus). A total of 36 free-living foxes were analyzed. Different skeletal muscles were assessed by microscopic and molecular methods. Cysts and/or DNA of Sarcocystis sp. were detected in 61.1% (22/36) foxes. Histopathology revealed the presence of sarcocysts in 52.8% (19/36) foxes. The tongue and masseter were the muscles more frequently infected. Of all the samples processed by homogenization of pooled muscles of each animal, 45.4% (10/22) evidenced muscle cysts and 68.2% (15/22) resulted positives by PCR. Individual cysts obtained from the ten positive samples in direct microscopic examination were all positive by PCR. Five amplicons from individual cysts from different samples were selected for sequencing together with four PCR products obtained from the pooled muscles. All nine sequences shared a high identity among them (99.8–100%) and showed the highest identity by BLAST (99%) with a S. svanai sequence (KM362428) from a North American dog. By transmission electron microscopy, the sarcocyst wall was thin (< 1 μm), had minute undulations, with tiny evaginations and without evident villar protrusions. The cyst wall type is referred as “type 1”. Sarcocystis svanai infects L. gymnocercus with a high prevalence and the presence of mature sarcocysts suggests the role of the Pampa. EEA Balcarce Fil: Scioscia, Nathalia Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Olmos, Leandro Hipolito. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Gorosabel, Antonella. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Bernad, Lucía. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Pedrana, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pedrana, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Hecker, Yanina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hecker, Yanina Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Gual, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gual, Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Gos, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gos, Maria Laura. Universidad Nacional de La Plata. Facultad de Ciencias Veterinaria; Argentina Fil: Denegri, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Moore, Dadin Prando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Moré, Gastón Andrés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinaria; Argentina 2024-09-30T11:41:15Z 2024-09-30T11:41:15Z 2017-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/19605 https://www.sciencedirect.com/science/article/pii/S1383576917300028 1383-5769 (Print) 1873-0329 (Online) https://doi.org/10.1016/j.parint.2017.01.021 eng info:eu-repograntAgreement/INTA/PNNAT-1128053/AR./Evaluación y manejo de la biodiversidad y sus servicios ecosistémicos de interés para la producción agropecuaria. info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Parasitology International 66 (3): 214-218 (June 2017) |
| spellingShingle | Histología Zorro Músculo Sarcocystis Histology Foxes Muscles Intermediate Hosts Huéspedes Intermediarios Lycalopex gymnocercus Sarcocystis svanai Scioscia, Nathalia Olmos, Leandro Hipolito Gorosabel, Antonella Bernad, Lucía Pedrana, Julieta Hecker, Yanina Gual, Ignacio Gos, Maria Laura Denegri, Guillermo Moore, Prando Dadin Moré, Gastón Andrés Pampas fox (Lycalopex gymnocercus) new intermediate host of Sarcocystis svanai (Apicomplexa: Sarcocystidae) |
| title | Pampas fox (Lycalopex gymnocercus) new intermediate host of Sarcocystis svanai (Apicomplexa: Sarcocystidae) |
| title_full | Pampas fox (Lycalopex gymnocercus) new intermediate host of Sarcocystis svanai (Apicomplexa: Sarcocystidae) |
| title_fullStr | Pampas fox (Lycalopex gymnocercus) new intermediate host of Sarcocystis svanai (Apicomplexa: Sarcocystidae) |
| title_full_unstemmed | Pampas fox (Lycalopex gymnocercus) new intermediate host of Sarcocystis svanai (Apicomplexa: Sarcocystidae) |
| title_short | Pampas fox (Lycalopex gymnocercus) new intermediate host of Sarcocystis svanai (Apicomplexa: Sarcocystidae) |
| title_sort | pampas fox lycalopex gymnocercus new intermediate host of sarcocystis svanai apicomplexa sarcocystidae |
| topic | Histología Zorro Músculo Sarcocystis Histology Foxes Muscles Intermediate Hosts Huéspedes Intermediarios Lycalopex gymnocercus Sarcocystis svanai |
| url | http://hdl.handle.net/20.500.12123/19605 https://www.sciencedirect.com/science/article/pii/S1383576917300028 https://doi.org/10.1016/j.parint.2017.01.021 |
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