Integration of transcriptomic and metabolic data reveals hub transcription factors involved in drought stress response in sunflower (Helianthus annuus L.)
Drought is one of the most important environmental stresses that effects crop productivity in many agricultural regions. Sunflower is tolerant to drought conditions but the mechanisms involved in this tolerance remain unclear at the molecular level. The aim of this study was to characterize and inte...
| Autores principales: | , , , , , , , , , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1960 https://link.springer.com/article/10.1007/s11103-017-0625-5 https://doi.org/10.1007/s11103-017-0625-5 |
| _version_ | 1855482970533527552 |
|---|---|
| author | Moschen, Sebastian Nicolas Di Rienzo, Julio A. Higgins, Janet Tohge, Takayuki Watanabe, Mutsumi Gonzalez, Sergio Alberto Rivarola, Maximo Lisandro García-García, Francisco Dopazo, Joaquín Hopp, Horacio Esteban Hoefgen, Rainer Fernie, Alisdair R. Paniego, Norma Beatriz Fernandez, Paula Del Carmen Heinz, Ruth Amelia |
| author_browse | Di Rienzo, Julio A. Dopazo, Joaquín Fernandez, Paula Del Carmen Fernie, Alisdair R. García-García, Francisco Gonzalez, Sergio Alberto Heinz, Ruth Amelia Higgins, Janet Hoefgen, Rainer Hopp, Horacio Esteban Moschen, Sebastian Nicolas Paniego, Norma Beatriz Rivarola, Maximo Lisandro Tohge, Takayuki Watanabe, Mutsumi |
| author_facet | Moschen, Sebastian Nicolas Di Rienzo, Julio A. Higgins, Janet Tohge, Takayuki Watanabe, Mutsumi Gonzalez, Sergio Alberto Rivarola, Maximo Lisandro García-García, Francisco Dopazo, Joaquín Hopp, Horacio Esteban Hoefgen, Rainer Fernie, Alisdair R. Paniego, Norma Beatriz Fernandez, Paula Del Carmen Heinz, Ruth Amelia |
| author_sort | Moschen, Sebastian Nicolas |
| collection | INTA Digital |
| description | Drought is one of the most important environmental stresses that effects crop productivity in many agricultural regions. Sunflower is tolerant to drought conditions but the mechanisms involved in this tolerance remain unclear at the molecular level. The aim of this study was to characterize and integrate transcriptional and metabolic pathways related to drought stress in sunflower plants, by using a system biology approach. Our results showed a delay in plant senescence with an increase in the expression level of photosynthesis related genes as well as higher levels of sugars, osmoprotectant amino acids and ionic nutrients under drought conditions. In addition, we identified transcription factors that were upregulated during drought conditions and that may act as hubs in the transcriptional network. Many of these transcription factors belong to families implicated in the drought response in model species. The integration of transcriptomic and metabolomic data in this study, together with physiological measurements, has improved our understanding of the biological responses during droughts and contributes to elucidate the molecular mechanisms involved under this environmental condition. These findings will provide useful biotechnological tools to improve stress tolerance while maintaining crop yield under restricted water availability. |
| format | Artículo |
| id | INTA1960 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA19602019-01-23T17:55:49Z Integration of transcriptomic and metabolic data reveals hub transcription factors involved in drought stress response in sunflower (Helianthus annuus L.) Moschen, Sebastian Nicolas Di Rienzo, Julio A. Higgins, Janet Tohge, Takayuki Watanabe, Mutsumi Gonzalez, Sergio Alberto Rivarola, Maximo Lisandro García-García, Francisco Dopazo, Joaquín Hopp, Horacio Esteban Hoefgen, Rainer Fernie, Alisdair R. Paniego, Norma Beatriz Fernandez, Paula Del Carmen Heinz, Ruth Amelia Helianthus Annuus Metabolismo Genética Sequía Estrés de Sequia Metabolism Genetics Drought Drought Stress Girasol Drought is one of the most important environmental stresses that effects crop productivity in many agricultural regions. Sunflower is tolerant to drought conditions but the mechanisms involved in this tolerance remain unclear at the molecular level. The aim of this study was to characterize and integrate transcriptional and metabolic pathways related to drought stress in sunflower plants, by using a system biology approach. Our results showed a delay in plant senescence with an increase in the expression level of photosynthesis related genes as well as higher levels of sugars, osmoprotectant amino acids and ionic nutrients under drought conditions. In addition, we identified transcription factors that were upregulated during drought conditions and that may act as hubs in the transcriptional network. Many of these transcription factors belong to families implicated in the drought response in model species. The integration of transcriptomic and metabolomic data in this study, together with physiological measurements, has improved our understanding of the biological responses during droughts and contributes to elucidate the molecular mechanisms involved under this environmental condition. These findings will provide useful biotechnological tools to improve stress tolerance while maintaining crop yield under restricted water availability. Instituto de Biotecnología Fil: Moschen, Sebastian Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Di Rienzo, Julio A.Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina Fil: Higgins, Janet. Norwich Research Park. Earlham Institute; Reino Unido Fil: Tohge, Takayuki. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania Fil: Watanabe, Mutsumi Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania Fil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: García-García, Francisco. Centro de Investigación Príncipe Felipe. Department of Bioinformatics and Genomics; España. Centro de Investigación Príncipe Felipe . National Institute of Bioinformatics. Functional Genomics Node; España Fil: Dopazo, Joaquín. Centro de Investigación Príncipe Felipe. Department of Bioinformatics and Genomics; España. Centro de Investigación Príncipe Felipe . National Institute of Bioinformatics. Functional Genomics Node; España Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Hoefgen, Rainer Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania Fil: Fernie, Alisdair R. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina 2018-03-05T15:34:08Z 2018-03-05T15:34:08Z 2017-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1960 https://link.springer.com/article/10.1007/s11103-017-0625-5 0167-4412 (Print) 1573-5028 (Online) https://doi.org/10.1007/s11103-017-0625-5 eng info:eu-repo/semantics/restrictedAccess application/pdf Plant molecular biology 94 (4–5) : 549–564. (July 2017) |
| spellingShingle | Helianthus Annuus Metabolismo Genética Sequía Estrés de Sequia Metabolism Genetics Drought Drought Stress Girasol Moschen, Sebastian Nicolas Di Rienzo, Julio A. Higgins, Janet Tohge, Takayuki Watanabe, Mutsumi Gonzalez, Sergio Alberto Rivarola, Maximo Lisandro García-García, Francisco Dopazo, Joaquín Hopp, Horacio Esteban Hoefgen, Rainer Fernie, Alisdair R. Paniego, Norma Beatriz Fernandez, Paula Del Carmen Heinz, Ruth Amelia Integration of transcriptomic and metabolic data reveals hub transcription factors involved in drought stress response in sunflower (Helianthus annuus L.) |
| title | Integration of transcriptomic and metabolic data reveals hub transcription factors involved in drought stress response in sunflower (Helianthus annuus L.) |
| title_full | Integration of transcriptomic and metabolic data reveals hub transcription factors involved in drought stress response in sunflower (Helianthus annuus L.) |
| title_fullStr | Integration of transcriptomic and metabolic data reveals hub transcription factors involved in drought stress response in sunflower (Helianthus annuus L.) |
| title_full_unstemmed | Integration of transcriptomic and metabolic data reveals hub transcription factors involved in drought stress response in sunflower (Helianthus annuus L.) |
| title_short | Integration of transcriptomic and metabolic data reveals hub transcription factors involved in drought stress response in sunflower (Helianthus annuus L.) |
| title_sort | integration of transcriptomic and metabolic data reveals hub transcription factors involved in drought stress response in sunflower helianthus annuus l |
| topic | Helianthus Annuus Metabolismo Genética Sequía Estrés de Sequia Metabolism Genetics Drought Drought Stress Girasol |
| url | http://hdl.handle.net/20.500.12123/1960 https://link.springer.com/article/10.1007/s11103-017-0625-5 https://doi.org/10.1007/s11103-017-0625-5 |
| work_keys_str_mv | AT moschensebastiannicolas integrationoftranscriptomicandmetabolicdatarevealshubtranscriptionfactorsinvolvedindroughtstressresponseinsunflowerhelianthusannuusl AT dirienzojulioa integrationoftranscriptomicandmetabolicdatarevealshubtranscriptionfactorsinvolvedindroughtstressresponseinsunflowerhelianthusannuusl AT higginsjanet integrationoftranscriptomicandmetabolicdatarevealshubtranscriptionfactorsinvolvedindroughtstressresponseinsunflowerhelianthusannuusl AT tohgetakayuki integrationoftranscriptomicandmetabolicdatarevealshubtranscriptionfactorsinvolvedindroughtstressresponseinsunflowerhelianthusannuusl AT watanabemutsumi integrationoftranscriptomicandmetabolicdatarevealshubtranscriptionfactorsinvolvedindroughtstressresponseinsunflowerhelianthusannuusl AT gonzalezsergioalberto integrationoftranscriptomicandmetabolicdatarevealshubtranscriptionfactorsinvolvedindroughtstressresponseinsunflowerhelianthusannuusl AT rivarolamaximolisandro integrationoftranscriptomicandmetabolicdatarevealshubtranscriptionfactorsinvolvedindroughtstressresponseinsunflowerhelianthusannuusl AT garciagarciafrancisco integrationoftranscriptomicandmetabolicdatarevealshubtranscriptionfactorsinvolvedindroughtstressresponseinsunflowerhelianthusannuusl AT dopazojoaquin integrationoftranscriptomicandmetabolicdatarevealshubtranscriptionfactorsinvolvedindroughtstressresponseinsunflowerhelianthusannuusl AT hopphoracioesteban integrationoftranscriptomicandmetabolicdatarevealshubtranscriptionfactorsinvolvedindroughtstressresponseinsunflowerhelianthusannuusl AT hoefgenrainer integrationoftranscriptomicandmetabolicdatarevealshubtranscriptionfactorsinvolvedindroughtstressresponseinsunflowerhelianthusannuusl AT ferniealisdairr integrationoftranscriptomicandmetabolicdatarevealshubtranscriptionfactorsinvolvedindroughtstressresponseinsunflowerhelianthusannuusl AT paniegonormabeatriz integrationoftranscriptomicandmetabolicdatarevealshubtranscriptionfactorsinvolvedindroughtstressresponseinsunflowerhelianthusannuusl AT fernandezpauladelcarmen integrationoftranscriptomicandmetabolicdatarevealshubtranscriptionfactorsinvolvedindroughtstressresponseinsunflowerhelianthusannuusl AT heinzruthamelia integrationoftranscriptomicandmetabolicdatarevealshubtranscriptionfactorsinvolvedindroughtstressresponseinsunflowerhelianthusannuusl |