Breed variability in the cellular mediated immune response to experimental Neospora caninum infection in heifers
Protozoan parasite Neospora caninum causes abortion in infected cattle while others remain asymptomatic. Host immunity plays a critical role in the outcome of bovine neosporosis. Despite extensive research, there is a critical gap in therapeutic and preventive measures, and no effective vaccines are...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Elsevier
2024
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/19512 https://www.sciencedirect.com/science/article/abs/pii/S0165242724001144 https://doi.org/10.1016/j.vetimm.2024.110828 |
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| author | Fiorani, Franco Dallard, Bibiana Cheuquepán Valenzuela, Felipe Andres Sosa, Emiliano Pardo, Alan Maxs Gual, Ignacio Morrell, Eleonora Lidia Marin, Maia Solange Quintana, Silvina Canton, German Jose Valentini, Beatriz Susana Echaide, Ignacio Eduardo Torioni, Susana Marta Cobo, Eduardo Rubén Corva, Pablo Moore, Prando Dadin |
| author_browse | Canton, German Jose Cheuquepán Valenzuela, Felipe Andres Cobo, Eduardo Rubén Corva, Pablo Dallard, Bibiana Echaide, Ignacio Eduardo Fiorani, Franco Gual, Ignacio Marin, Maia Solange Moore, Prando Dadin Morrell, Eleonora Lidia Pardo, Alan Maxs Quintana, Silvina Sosa, Emiliano Torioni, Susana Marta Valentini, Beatriz Susana |
| author_facet | Fiorani, Franco Dallard, Bibiana Cheuquepán Valenzuela, Felipe Andres Sosa, Emiliano Pardo, Alan Maxs Gual, Ignacio Morrell, Eleonora Lidia Marin, Maia Solange Quintana, Silvina Canton, German Jose Valentini, Beatriz Susana Echaide, Ignacio Eduardo Torioni, Susana Marta Cobo, Eduardo Rubén Corva, Pablo Moore, Prando Dadin |
| author_sort | Fiorani, Franco |
| collection | INTA Digital |
| description | Protozoan parasite Neospora caninum causes abortion in infected cattle while others remain asymptomatic. Host immunity plays a critical role in the outcome of bovine neosporosis. Despite extensive research, there is a critical gap in therapeutic and preventive measures, and no effective vaccines are available. Both beef and dairy cattle can suffer from N. caninum-induced abortions, but cumulative evidence suggests a breed susceptibility being higher in dairy compared with beef breeds. It has been established that the response to N. caninum infection primarily involves a cell-mediated immune response (CMIR) regulated by T-helper type 1 (Th1) cells and specific cytokines. The delayed-type hypersensitivity (DTH) skin test has been used to measure the ability of livestock to generate CMIR, in the context of breeding for disease resistance and as a method for diagnosis of several diseases. In this study, we evaluated the immune response triggered by an N. caninum-induced DTH skin test between Holstein – a dairy breed intensively selected- and Argentinean Creole heifers – a beef breed with minimal genetic selection- to assess differences in CMIR following experimental N. caninum infection. The immune response, measured through skinfold thickness and histological and immune molecular analysis, revealed variations between the breeds. Our study found an increased CMIR in Argentinean Creole heifers compared to Holstein heifers. Differential gene expression of key cytokines was observed at the DTH skin test site. Argentinean Creole heifers exhibited elevated IFN-γ, IL-12, IL-10, and IL-4, while Holstein heifers only showed higher expression of IL-17. This finding could underscore genetic diversity in response to neosporosis, which could be used in breeding cattle strategies for disease resistance in cattle populations. |
| format | Artículo |
| id | INTA19512 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA195122024-09-24T11:29:20Z Breed variability in the cellular mediated immune response to experimental Neospora caninum infection in heifers Fiorani, Franco Dallard, Bibiana Cheuquepán Valenzuela, Felipe Andres Sosa, Emiliano Pardo, Alan Maxs Gual, Ignacio Morrell, Eleonora Lidia Marin, Maia Solange Quintana, Silvina Canton, German Jose Valentini, Beatriz Susana Echaide, Ignacio Eduardo Torioni, Susana Marta Cobo, Eduardo Rubén Corva, Pablo Moore, Prando Dadin Neospora caninum Respuesta Inmunológica Razas de Ganado Prueba Cutánea Hipersensibilidad Retardada Neosporosis Immune Response Livestock Breeds Skin Tests Delayed Hypersensitivity Protozoan parasite Neospora caninum causes abortion in infected cattle while others remain asymptomatic. Host immunity plays a critical role in the outcome of bovine neosporosis. Despite extensive research, there is a critical gap in therapeutic and preventive measures, and no effective vaccines are available. Both beef and dairy cattle can suffer from N. caninum-induced abortions, but cumulative evidence suggests a breed susceptibility being higher in dairy compared with beef breeds. It has been established that the response to N. caninum infection primarily involves a cell-mediated immune response (CMIR) regulated by T-helper type 1 (Th1) cells and specific cytokines. The delayed-type hypersensitivity (DTH) skin test has been used to measure the ability of livestock to generate CMIR, in the context of breeding for disease resistance and as a method for diagnosis of several diseases. In this study, we evaluated the immune response triggered by an N. caninum-induced DTH skin test between Holstein – a dairy breed intensively selected- and Argentinean Creole heifers – a beef breed with minimal genetic selection- to assess differences in CMIR following experimental N. caninum infection. The immune response, measured through skinfold thickness and histological and immune molecular analysis, revealed variations between the breeds. Our study found an increased CMIR in Argentinean Creole heifers compared to Holstein heifers. Differential gene expression of key cytokines was observed at the DTH skin test site. Argentinean Creole heifers exhibited elevated IFN-γ, IL-12, IL-10, and IL-4, while Holstein heifers only showed higher expression of IL-17. This finding could underscore genetic diversity in response to neosporosis, which could be used in breeding cattle strategies for disease resistance in cattle populations. EEA Balcarce Fil: Fiorani, Franco. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Dallard, Bibiana. Universidad Nacional del Litoral. Facultad de Medicina Veterinaria; Argentina Fil: Cheuquepán Valenzuela, Felipe Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Sosa, Emiliano. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Pardo, Alan Maxs. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Pardo, Alan Maxs. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Gual, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Gual, Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Marin, Maia Solange. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Quintana, S. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Valentini, Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Echaide, Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Torioni, S.M. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Cobo, E.R. University of Calgary. Faculty of Veterinary Medicine; Canadá Fil: Corva, Pablo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Moore, Dadín Prando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Moore. Dadín Prando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina 2024-09-24T11:13:24Z 2024-09-24T11:13:24Z 2024-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/19512 https://www.sciencedirect.com/science/article/abs/pii/S0165242724001144 1873-2534 (online) 0165-2427 (print) https://doi.org/10.1016/j.vetimm.2024.110828 eng info:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Salud info:eu-repograntAgreement/INTA/2023-PD-L06-I116, Implementación de tecnologías y nuevas estrategias preventivas y terapéuticas para el desarrollo sustentable y eficiente de la producción animal en el marco de Una Salud info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Veterinary Immunology and Immunopathology 276 : 110828 (October 2024) |
| spellingShingle | Neospora caninum Respuesta Inmunológica Razas de Ganado Prueba Cutánea Hipersensibilidad Retardada Neosporosis Immune Response Livestock Breeds Skin Tests Delayed Hypersensitivity Fiorani, Franco Dallard, Bibiana Cheuquepán Valenzuela, Felipe Andres Sosa, Emiliano Pardo, Alan Maxs Gual, Ignacio Morrell, Eleonora Lidia Marin, Maia Solange Quintana, Silvina Canton, German Jose Valentini, Beatriz Susana Echaide, Ignacio Eduardo Torioni, Susana Marta Cobo, Eduardo Rubén Corva, Pablo Moore, Prando Dadin Breed variability in the cellular mediated immune response to experimental Neospora caninum infection in heifers |
| title | Breed variability in the cellular mediated immune response to experimental Neospora caninum infection in heifers |
| title_full | Breed variability in the cellular mediated immune response to experimental Neospora caninum infection in heifers |
| title_fullStr | Breed variability in the cellular mediated immune response to experimental Neospora caninum infection in heifers |
| title_full_unstemmed | Breed variability in the cellular mediated immune response to experimental Neospora caninum infection in heifers |
| title_short | Breed variability in the cellular mediated immune response to experimental Neospora caninum infection in heifers |
| title_sort | breed variability in the cellular mediated immune response to experimental neospora caninum infection in heifers |
| topic | Neospora caninum Respuesta Inmunológica Razas de Ganado Prueba Cutánea Hipersensibilidad Retardada Neosporosis Immune Response Livestock Breeds Skin Tests Delayed Hypersensitivity |
| url | http://hdl.handle.net/20.500.12123/19512 https://www.sciencedirect.com/science/article/abs/pii/S0165242724001144 https://doi.org/10.1016/j.vetimm.2024.110828 |
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