Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptake
Relationship between fluazuron (FZN) concentrations in cattle plasma and ticks and its therapeutic efficacy percentage (EP) against Rhipicephalus microplus was analyzed. The extent of FZN uptake by ticks after its topical administration was also evaluated. Heifers, naturally infested with R. micropl...
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/19511 https://www.sciencedirect.com/science/article/pii/S0034528824002698 https://doi.org/10.1016/j.rvsc.2024.105402 |
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| author | Sarli, Macarena Miró, María Victoria Rossner, Maria Victoria Gomez, Viviana Daniela Torrents, Jorgelina Nava, Santiago Lifschitz, Adrián Luis |
| author_browse | Gomez, Viviana Daniela Lifschitz, Adrián Luis Miró, María Victoria Nava, Santiago Rossner, Maria Victoria Sarli, Macarena Torrents, Jorgelina |
| author_facet | Sarli, Macarena Miró, María Victoria Rossner, Maria Victoria Gomez, Viviana Daniela Torrents, Jorgelina Nava, Santiago Lifschitz, Adrián Luis |
| author_sort | Sarli, Macarena |
| collection | INTA Digital |
| description | Relationship between fluazuron (FZN) concentrations in cattle plasma and ticks and its therapeutic efficacy percentage (EP) against Rhipicephalus microplus was analyzed. The extent of FZN uptake by ticks after its topical administration was also evaluated. Heifers, naturally infested with R. microplus, were divided in treated and no treated groups. Blood and tick samples were taken to measure FZN concentrations and female ticks were counted to evaluate the EP of the treatment from day 0 to 45 post-treatment (PT). Ticks exposed (TE) and not exposed (TNE) to topical contact with the drug were collected at 12 and 24 h PT. Artificial feeding (AF) of ticks was also performed with blood from a treated heifer. The EP on days 21, 28, 35, 42 and 45 PT was 99.1, 92.3, 81.2, 74.7 and 76.5 %, respectively. Fluazuron disposition within ticks during the first 2 days post-treatment did not mirror the corresponding FZN levels in the bovine plasma, but a statistically significant positive correlation was evident from day 3. Mean FZN concentrations in TE at 12 h and 24 h PT were significantly higher than in TNE. The FZN concentrations in AF ticks were significantly lower than those in TE but comparable to the levels in TNE. The efficacy and pharmacokinetics analysis suggest that maintaining a threshold FZN plasma concentration above 20–25 ng/mL is crucial to prevent the development of larvae into engorged females. The results also indicate that direct absorption of FZN through the tick's integument occurs in the initial days after treatment. |
| format | Artículo |
| id | INTA19511 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA195112024-09-24T11:07:49Z Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptake Sarli, Macarena Miró, María Victoria Rossner, Maria Victoria Gomez, Viviana Daniela Torrents, Jorgelina Nava, Santiago Lifschitz, Adrián Luis Cattle Acaricides Drugs Pharmacokinetics Pharmacodynamics Ganado Bovino Acaricidas Medicamentos Farmacocinética Farmacodinámica Rhipicephalus microplus Fluazurón Control de Garrapatas Fluazuron Tick Control Relationship between fluazuron (FZN) concentrations in cattle plasma and ticks and its therapeutic efficacy percentage (EP) against Rhipicephalus microplus was analyzed. The extent of FZN uptake by ticks after its topical administration was also evaluated. Heifers, naturally infested with R. microplus, were divided in treated and no treated groups. Blood and tick samples were taken to measure FZN concentrations and female ticks were counted to evaluate the EP of the treatment from day 0 to 45 post-treatment (PT). Ticks exposed (TE) and not exposed (TNE) to topical contact with the drug were collected at 12 and 24 h PT. Artificial feeding (AF) of ticks was also performed with blood from a treated heifer. The EP on days 21, 28, 35, 42 and 45 PT was 99.1, 92.3, 81.2, 74.7 and 76.5 %, respectively. Fluazuron disposition within ticks during the first 2 days post-treatment did not mirror the corresponding FZN levels in the bovine plasma, but a statistically significant positive correlation was evident from day 3. Mean FZN concentrations in TE at 12 h and 24 h PT were significantly higher than in TNE. The FZN concentrations in AF ticks were significantly lower than those in TE but comparable to the levels in TNE. The efficacy and pharmacokinetics analysis suggest that maintaining a threshold FZN plasma concentration above 20–25 ng/mL is crucial to prevent the development of larvae into engorged females. The results also indicate that direct absorption of FZN through the tick's integument occurs in the initial days after treatment. Asociacion Cooperadora INTA Rafaela. Agencia Nacional de Promocion Científica y Tecnologica (PICT-2021-0278). Agencia Santafesina de Ciencia, Tecnología e Innovacion (PEICID2022-049). Secretaría de Innovacion, Ciencia y Tecnología (Proyectos de Redes Federales de Alto Impacto: REDGARR 100390265) EEA Rafaela Fil: Sarli, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Miró, V. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina. Fil: Miró, V. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Rossner, Maria Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benitez; Argentina Fil: Gomez, Viviana Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benitez; Argentina Fil: Torrents, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Torrents, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Lifschitz, Adrian. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN). Laboratorio de Farmacología; Argentina. Fil: Lifschitz, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina 2024-09-24T10:56:03Z 2024-09-24T10:56:03Z 2024-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/19511 https://www.sciencedirect.com/science/article/pii/S0034528824002698 0034-5288 https://doi.org/10.1016/j.rvsc.2024.105402 eng info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001, Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Research in Veterinary Science 180 : 105402 (November 2024) |
| spellingShingle | Cattle Acaricides Drugs Pharmacokinetics Pharmacodynamics Ganado Bovino Acaricidas Medicamentos Farmacocinética Farmacodinámica Rhipicephalus microplus Fluazurón Control de Garrapatas Fluazuron Tick Control Sarli, Macarena Miró, María Victoria Rossner, Maria Victoria Gomez, Viviana Daniela Torrents, Jorgelina Nava, Santiago Lifschitz, Adrián Luis Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptake |
| title | Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptake |
| title_full | Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptake |
| title_fullStr | Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptake |
| title_full_unstemmed | Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptake |
| title_short | Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptake |
| title_sort | relationship between pharmacokinetics of fluazuron and its efficacy for controlling rhipicephalus microplus a comprehensive evaluation of tick drug uptake |
| topic | Cattle Acaricides Drugs Pharmacokinetics Pharmacodynamics Ganado Bovino Acaricidas Medicamentos Farmacocinética Farmacodinámica Rhipicephalus microplus Fluazurón Control de Garrapatas Fluazuron Tick Control |
| url | http://hdl.handle.net/20.500.12123/19511 https://www.sciencedirect.com/science/article/pii/S0034528824002698 https://doi.org/10.1016/j.rvsc.2024.105402 |
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