Nanogel-Mediated antigen delivery: Biocompatibility, immunogenicity, and potential for tailored vaccine design across species
Nanotechnology has emerged as a promising avenue for enhancing the efficacy of vaccine delivery systems. This study investigates the utilization of nanogels as carriers for the model antigen ovalbumin, with a focus on in vivo assessments in equine and murine models. Nanogels, owing to their biocompa...
| Main Authors: | , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/19368 https://www.sciencedirect.com/science/article/abs/pii/S0264410X24005255 https://doi.org/10.1016/j.vaccine.2024.04.086 |
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| author | Soriano Perez, María Laura Montironi, Ivana D. Funes, Javier Alejandro Margineda, Carlos Augusto Campra, Noelia Cariddi, Laura Noelia Garrido, Juan José Molina, María Alustiza, Fabrisio Eduardo |
| author_browse | Alustiza, Fabrisio Eduardo Campra, Noelia Cariddi, Laura Noelia Funes, Javier Alejandro Garrido, Juan José Margineda, Carlos Augusto Molina, María Montironi, Ivana D. Soriano Perez, María Laura |
| author_facet | Soriano Perez, María Laura Montironi, Ivana D. Funes, Javier Alejandro Margineda, Carlos Augusto Campra, Noelia Cariddi, Laura Noelia Garrido, Juan José Molina, María Alustiza, Fabrisio Eduardo |
| author_sort | Soriano Perez, María Laura |
| collection | INTA Digital |
| description | Nanotechnology has emerged as a promising avenue for enhancing the efficacy of vaccine delivery systems. This study investigates the utilization of nanogels as carriers for the model antigen ovalbumin, with a focus on in vivo assessments in equine and murine models. Nanogels, owing to their biocompatibility and tunable physicochemical properties, offer a versatile platform for efficient antigen encapsulation and controlled release. The encapsulation efficiency and physicochemical characteristics of ovalbumin-loaded nanogels were comprehensively characterized. In vitro biocompatibility was evaluated, finding excellent properties of these nanogels. In vivo evaluations were conducted on both equine and murine subjects, assessing immunogenicity through antibody and splenic cell response. Furthermore, the study propose the potential use of nanogels in tailoring immune responses through the modulation of antigen release kinetics. The results obtained in the in vitro assays showed an increase in the uptake of nanogels by APCs compared to free antigen (OVA). In mice, an absence of inflammatory response in the inoculation site was observed, without systemic damage in the evaluated organs. In addition, non-significant humoral response was found nor cellular proliferation and proinflammatory cytokine production, compared with a traditional adjuvant as aluminum hydroxide, in both animal models. These findings allow further insights
into nanogel-based delivery systems and offer valuable insights into their application in various animal models. In conclusion, this research establishes the utility of nanogels as effective carriers for antigens-based vaccines, with interesting biocompatibility properties and highly taken affinity by antigen-presenting cells, without inducing inflammation at the injection site. The study underscores the potential of nanogel technology in revolutionizing vaccine design and highlights the importance of tailored approaches for diverse target species. |
| format | info:ar-repo/semantics/artículo |
| id | INTA19368 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA193682025-07-21T15:04:44Z Nanogel-Mediated antigen delivery: Biocompatibility, immunogenicity, and potential for tailored vaccine design across species Soriano Perez, María Laura Montironi, Ivana D. Funes, Javier Alejandro Margineda, Carlos Augusto Campra, Noelia Cariddi, Laura Noelia Garrido, Juan José Molina, María Alustiza, Fabrisio Eduardo Nanotechnology Vaccines Nanomedicine Nanobiotechnology Nanotecnología Vacuna Nanobiotecnología Nanomedicina Nanogeles Nanotechnology has emerged as a promising avenue for enhancing the efficacy of vaccine delivery systems. This study investigates the utilization of nanogels as carriers for the model antigen ovalbumin, with a focus on in vivo assessments in equine and murine models. Nanogels, owing to their biocompatibility and tunable physicochemical properties, offer a versatile platform for efficient antigen encapsulation and controlled release. The encapsulation efficiency and physicochemical characteristics of ovalbumin-loaded nanogels were comprehensively characterized. In vitro biocompatibility was evaluated, finding excellent properties of these nanogels. In vivo evaluations were conducted on both equine and murine subjects, assessing immunogenicity through antibody and splenic cell response. Furthermore, the study propose the potential use of nanogels in tailoring immune responses through the modulation of antigen release kinetics. The results obtained in the in vitro assays showed an increase in the uptake of nanogels by APCs compared to free antigen (OVA). In mice, an absence of inflammatory response in the inoculation site was observed, without systemic damage in the evaluated organs. In addition, non-significant humoral response was found nor cellular proliferation and proinflammatory cytokine production, compared with a traditional adjuvant as aluminum hydroxide, in both animal models. These findings allow further insights into nanogel-based delivery systems and offer valuable insights into their application in various animal models. In conclusion, this research establishes the utility of nanogels as effective carriers for antigens-based vaccines, with interesting biocompatibility properties and highly taken affinity by antigen-presenting cells, without inducing inflammation at the injection site. The study underscores the potential of nanogel technology in revolutionizing vaccine design and highlights the importance of tailored approaches for diverse target species. EEA Marcos Juárez, INTA Fil: Soriano Pérez, María Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Montironi, Ivana D. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología, Instituto de Biotecnología Ambiental y Salud (INBIAS-CONICET); Argentina Fil: Funes, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Margineda, Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Campra, Noelia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología, Instituto de Biotecnología Ambiental y Salud (INBIAS-CONICET); Argentina Fil: Cariddi, Laura Noelia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología, Instituto de Biotecnología Ambiental y Salud (INBIAS-CONICET); Argentina Fil: Garrido, Juan José. Universidad de Córdoba. Facultad de Medicina Veterinaria. Laboratorio de Inmunogenómica y Patogénesis Molecular, Departamento de Genética; España Fil: Molina, María. Universidad Nacional de Río Cuarto. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados (IITEMA), Argentina Fil: Alustiza, Fabrisio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina 2024-09-12T11:51:18Z 2024-09-12T11:51:18Z 2024-06-20 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/19368 https://www.sciencedirect.com/science/article/abs/pii/S0264410X24005255 0264 410X https://doi.org/10.1016/j.vaccine.2024.04.086 eng info:eu-repograntAgreement/INTA/2023-PD-L06-I116, Implementación de tecnologías y nuevas estrategias preventivas y terapéuticas para el desarrollo sustentable y eficiente de la producción animal en el marco de Una Salud info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Vaccine 42 (17) : 3721-3732. (June 2024) |
| spellingShingle | Nanotechnology Vaccines Nanomedicine Nanobiotechnology Nanotecnología Vacuna Nanobiotecnología Nanomedicina Nanogeles Soriano Perez, María Laura Montironi, Ivana D. Funes, Javier Alejandro Margineda, Carlos Augusto Campra, Noelia Cariddi, Laura Noelia Garrido, Juan José Molina, María Alustiza, Fabrisio Eduardo Nanogel-Mediated antigen delivery: Biocompatibility, immunogenicity, and potential for tailored vaccine design across species |
| title | Nanogel-Mediated antigen delivery: Biocompatibility, immunogenicity, and potential for tailored vaccine design across species |
| title_full | Nanogel-Mediated antigen delivery: Biocompatibility, immunogenicity, and potential for tailored vaccine design across species |
| title_fullStr | Nanogel-Mediated antigen delivery: Biocompatibility, immunogenicity, and potential for tailored vaccine design across species |
| title_full_unstemmed | Nanogel-Mediated antigen delivery: Biocompatibility, immunogenicity, and potential for tailored vaccine design across species |
| title_short | Nanogel-Mediated antigen delivery: Biocompatibility, immunogenicity, and potential for tailored vaccine design across species |
| title_sort | nanogel mediated antigen delivery biocompatibility immunogenicity and potential for tailored vaccine design across species |
| topic | Nanotechnology Vaccines Nanomedicine Nanobiotechnology Nanotecnología Vacuna Nanobiotecnología Nanomedicina Nanogeles |
| url | http://hdl.handle.net/20.500.12123/19368 https://www.sciencedirect.com/science/article/abs/pii/S0264410X24005255 https://doi.org/10.1016/j.vaccine.2024.04.086 |
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