Positive selection analysis of Cyt proteins from Bacillus thuringiensis: a conservative trend driven by negative (purifying) selection
Bacillus thuringiensis is a Gram-positive entomopathogenic bacterium that produces different pesticidal proteins: vegetative insecticidal proteins (Vpb1/Vpa2, Vip3, and Vpb4) during vegetative growth, which are secreted to the culture medium, and δ–endotoxins (Cry and Cyt) during sporulation, which...
| Main Authors: | , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2024
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/19360 https://www.sciencedirect.com/science/article/abs/pii/S0041010124004252 https://doi.org/10.1016/j.toxicon.2024.107853 |
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| author | Palma, Leopoldo Sauka, Diego Herman Berry, Colin Peralta, Cecilia |
| author_browse | Berry, Colin Palma, Leopoldo Peralta, Cecilia Sauka, Diego Herman |
| author_facet | Palma, Leopoldo Sauka, Diego Herman Berry, Colin Peralta, Cecilia |
| author_sort | Palma, Leopoldo |
| collection | INTA Digital |
| description | Bacillus thuringiensis is a Gram-positive entomopathogenic bacterium that produces different pesticidal proteins: vegetative insecticidal proteins (Vpb1/Vpa2, Vip3, and Vpb4) during vegetative growth, which are secreted to the culture medium, and δ–endotoxins (Cry and Cyt) during sporulation, which accumulate into parasporal crystals. Cyt proteins are the smaller subset of δ–endotoxins targeting Diptera species. While Cry and Vip3 proteins undergo positive selection, our analysis suggests that Cyt proteins evolve following a conservative trend driven negative (purifying) selection. |
| format | info:ar-repo/semantics/artículo |
| id | INTA19360 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA193602024-09-12T10:32:19Z Positive selection analysis of Cyt proteins from Bacillus thuringiensis: a conservative trend driven by negative (purifying) selection Palma, Leopoldo Sauka, Diego Herman Berry, Colin Peralta, Cecilia Bacillus thuringiensis Control Biológico Biological Control Pesticides Plaguicidas Cyt proteins pesticidas bacteria entomopatógena Biocontrol Bacillus thuringiensis is a Gram-positive entomopathogenic bacterium that produces different pesticidal proteins: vegetative insecticidal proteins (Vpb1/Vpa2, Vip3, and Vpb4) during vegetative growth, which are secreted to the culture medium, and δ–endotoxins (Cry and Cyt) during sporulation, which accumulate into parasporal crystals. Cyt proteins are the smaller subset of δ–endotoxins targeting Diptera species. While Cry and Vip3 proteins undergo positive selection, our analysis suggests that Cyt proteins evolve following a conservative trend driven negative (purifying) selection. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Palma, Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Palma, Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB); Argentina. Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB); Argentina Fil: Palma, Leopoldo. Universitat de València. Departamento de Genética. Instituto BIOTECMED. Laboratorio de Control Biotecnológico de Plagas; España Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Berry, Colin. Cardiff University. Cardiff School of Biosciences; Reino Unido Fil: Peralta, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Peralta, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB); Argentina. Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB); Argentina Fil: Peralta, Cecilia. Universitat de València. Departamento de Genética. Instituto BIOTECMED. Laboratorio de Control Biotecnológico de Plagas; España 2024-09-12T10:23:47Z 2024-09-12T10:23:47Z 2024-08-28 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/19360 https://www.sciencedirect.com/science/article/abs/pii/S0041010124004252 1879-3150 0041-0101 https://doi.org/10.1016/j.toxicon.2024.107853 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Toxicon 247 (1) : 107853. (28 August 2024) |
| spellingShingle | Bacillus thuringiensis Control Biológico Biological Control Pesticides Plaguicidas Cyt proteins pesticidas bacteria entomopatógena Biocontrol Palma, Leopoldo Sauka, Diego Herman Berry, Colin Peralta, Cecilia Positive selection analysis of Cyt proteins from Bacillus thuringiensis: a conservative trend driven by negative (purifying) selection |
| title | Positive selection analysis of Cyt proteins from Bacillus thuringiensis: a conservative trend driven by negative (purifying) selection |
| title_full | Positive selection analysis of Cyt proteins from Bacillus thuringiensis: a conservative trend driven by negative (purifying) selection |
| title_fullStr | Positive selection analysis of Cyt proteins from Bacillus thuringiensis: a conservative trend driven by negative (purifying) selection |
| title_full_unstemmed | Positive selection analysis of Cyt proteins from Bacillus thuringiensis: a conservative trend driven by negative (purifying) selection |
| title_short | Positive selection analysis of Cyt proteins from Bacillus thuringiensis: a conservative trend driven by negative (purifying) selection |
| title_sort | positive selection analysis of cyt proteins from bacillus thuringiensis a conservative trend driven by negative purifying selection |
| topic | Bacillus thuringiensis Control Biológico Biological Control Pesticides Plaguicidas Cyt proteins pesticidas bacteria entomopatógena Biocontrol |
| url | http://hdl.handle.net/20.500.12123/19360 https://www.sciencedirect.com/science/article/abs/pii/S0041010124004252 https://doi.org/10.1016/j.toxicon.2024.107853 |
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