Quantitative Proteomics Analysis of ABA- and GA3-Treated Malbec Berries Reveals Insights into H2O2 Scavenging and Anthocyanin Dynamics
Abscisic acid (ABA) and gibberellic acid (GA3) are regulators of fruit color and sugar levels, and the application of these hormones is a common practice in commercial vineyards dedicated to the production of table grapes. However, the effects of exogenous ABA and GA3 on wine cultivars remain unclea...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/19213 https://www.mdpi.com/2223-7747/13/17/2366 https://doi.org/10.3390/plants13172366 |
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| author | Murcia, Mauro Germán Alonso, Rodrigo Berli, Federico Javier Arias, Leonardo Bianchimano, Luciana Pontin, Mariela Ana Fontana, Ariel Casal, Jorge José Piccoli, Patricia Noemí |
| author_browse | Alonso, Rodrigo Arias, Leonardo Berli, Federico Javier Bianchimano, Luciana Casal, Jorge José Fontana, Ariel Murcia, Mauro Germán Piccoli, Patricia Noemí Pontin, Mariela Ana |
| author_facet | Murcia, Mauro Germán Alonso, Rodrigo Berli, Federico Javier Arias, Leonardo Bianchimano, Luciana Pontin, Mariela Ana Fontana, Ariel Casal, Jorge José Piccoli, Patricia Noemí |
| author_sort | Murcia, Mauro Germán |
| collection | INTA Digital |
| description | Abscisic acid (ABA) and gibberellic acid (GA3) are regulators of fruit color and sugar levels, and the application of these hormones is a common practice in commercial vineyards dedicated to the production of table grapes. However, the effects of exogenous ABA and GA3 on wine cultivars remain unclear. We investigated the impact of ABA and GA3 application on Malbec grapevine berries across three developmental stages. We found similar patterns of berry total anthocyanin accumulation induced by both treatments, closely associated with berry H2O2 levels. Quantitative proteomics from berry skins revealed that ABA and GA3 positively modulated antioxidant defense proteins, mitigating H2O2. Consequently, proteins involved in phenylpropanoid biosynthesis were downregulated, leading to decreased anthocyanin content at the almost ripe stage, particularly petunidin-3-G and peonidin-3-G. Additionally, we noted increased levels of the non-anthocyanins E-viniferin and quercetin in the treated berries, which may enhance H2O2 scavenging at the almost ripe stage. Using a linear mixed-effects model, we found statistical significance for fixed effects including the berry H2O2 and sugar contents, demonstrating their roles in anthocyanin accumulation. In conclusion, our findings suggest a common molecular mechanism by which ABA and GA3 influence berry H2O2 content, ultimately impacting anthocyanin dynamics during ripening. |
| format | Artículo |
| id | INTA19213 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA192132024-09-02T13:58:09Z Quantitative Proteomics Analysis of ABA- and GA3-Treated Malbec Berries Reveals Insights into H2O2 Scavenging and Anthocyanin Dynamics Murcia, Mauro Germán Alonso, Rodrigo Berli, Federico Javier Arias, Leonardo Bianchimano, Luciana Pontin, Mariela Ana Fontana, Ariel Casal, Jorge José Piccoli, Patricia Noemí Vid Proteómica Antocianinas Ácido Giberelico Peróxido de Hidrógeno Grapevines ABA Proteomics Anthocyanins Gibberellic Acid Hydrogen Peroxide Malbec Ácido Abscísico Agua Oxigenada Abscisic Acid Abscisic acid (ABA) and gibberellic acid (GA3) are regulators of fruit color and sugar levels, and the application of these hormones is a common practice in commercial vineyards dedicated to the production of table grapes. However, the effects of exogenous ABA and GA3 on wine cultivars remain unclear. We investigated the impact of ABA and GA3 application on Malbec grapevine berries across three developmental stages. We found similar patterns of berry total anthocyanin accumulation induced by both treatments, closely associated with berry H2O2 levels. Quantitative proteomics from berry skins revealed that ABA and GA3 positively modulated antioxidant defense proteins, mitigating H2O2. Consequently, proteins involved in phenylpropanoid biosynthesis were downregulated, leading to decreased anthocyanin content at the almost ripe stage, particularly petunidin-3-G and peonidin-3-G. Additionally, we noted increased levels of the non-anthocyanins E-viniferin and quercetin in the treated berries, which may enhance H2O2 scavenging at the almost ripe stage. Using a linear mixed-effects model, we found statistical significance for fixed effects including the berry H2O2 and sugar contents, demonstrating their roles in anthocyanin accumulation. In conclusion, our findings suggest a common molecular mechanism by which ABA and GA3 influence berry H2O2 content, ultimately impacting anthocyanin dynamics during ripening. EEA La Consulta Fil: Murcia, Mauro Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Alonso, Rodrigo. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Alonso, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Berli, Federico Javier. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Arias, Leonardo. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Arias, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Bianchimano, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Pontin, Mariela Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina Fil: Fontana, Ariel. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Fontana, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Casal, Jorge José. Consejo Nacional de Investigaciones Científicas y Técnicas. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Casal, Jorge José. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina Fil: Casal, Jorge José. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina Fil: Piccoli, Patricia Noemí. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina 2024-09-02T13:53:53Z 2024-09-02T13:53:53Z 2024-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/19213 https://www.mdpi.com/2223-7747/13/17/2366 2223-7747 https://doi.org/10.3390/plants13172366 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Plants 13 (17) : 2366. (September 2024) |
| spellingShingle | Vid Proteómica Antocianinas Ácido Giberelico Peróxido de Hidrógeno Grapevines ABA Proteomics Anthocyanins Gibberellic Acid Hydrogen Peroxide Malbec Ácido Abscísico Agua Oxigenada Abscisic Acid Murcia, Mauro Germán Alonso, Rodrigo Berli, Federico Javier Arias, Leonardo Bianchimano, Luciana Pontin, Mariela Ana Fontana, Ariel Casal, Jorge José Piccoli, Patricia Noemí Quantitative Proteomics Analysis of ABA- and GA3-Treated Malbec Berries Reveals Insights into H2O2 Scavenging and Anthocyanin Dynamics |
| title | Quantitative Proteomics Analysis of ABA- and GA3-Treated Malbec Berries Reveals Insights into H2O2 Scavenging and Anthocyanin Dynamics |
| title_full | Quantitative Proteomics Analysis of ABA- and GA3-Treated Malbec Berries Reveals Insights into H2O2 Scavenging and Anthocyanin Dynamics |
| title_fullStr | Quantitative Proteomics Analysis of ABA- and GA3-Treated Malbec Berries Reveals Insights into H2O2 Scavenging and Anthocyanin Dynamics |
| title_full_unstemmed | Quantitative Proteomics Analysis of ABA- and GA3-Treated Malbec Berries Reveals Insights into H2O2 Scavenging and Anthocyanin Dynamics |
| title_short | Quantitative Proteomics Analysis of ABA- and GA3-Treated Malbec Berries Reveals Insights into H2O2 Scavenging and Anthocyanin Dynamics |
| title_sort | quantitative proteomics analysis of aba and ga3 treated malbec berries reveals insights into h2o2 scavenging and anthocyanin dynamics |
| topic | Vid Proteómica Antocianinas Ácido Giberelico Peróxido de Hidrógeno Grapevines ABA Proteomics Anthocyanins Gibberellic Acid Hydrogen Peroxide Malbec Ácido Abscísico Agua Oxigenada Abscisic Acid |
| url | http://hdl.handle.net/20.500.12123/19213 https://www.mdpi.com/2223-7747/13/17/2366 https://doi.org/10.3390/plants13172366 |
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