The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling model
Azospirillum sp. is one of the most studied genera of plant growth-promoting rhizobacteria (PGPR). The ability of Azospirillum sp. to promote plant growth has been associated with its ability to produce several phytohormones, such as auxins, gibberellins and cytokinins, but mainly indole-3-acetic ac...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1911 https://link.springer.com/article/10.1007/s13199-017-0536-x https://doi.org/10.1007/s13199-017-0536-x |
| _version_ | 1855482962416500736 |
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| author | Puente, Mariana Laura Gualpa, Jose Luis López, Gastón A. Molina, Romina M. Carletti, Susana Cassán, Fabricio D. |
| author_browse | Carletti, Susana Cassán, Fabricio D. Gualpa, Jose Luis López, Gastón A. Molina, Romina M. Puente, Mariana Laura |
| author_facet | Puente, Mariana Laura Gualpa, Jose Luis López, Gastón A. Molina, Romina M. Carletti, Susana Cassán, Fabricio D. |
| author_sort | Puente, Mariana Laura |
| collection | INTA Digital |
| description | Azospirillum sp. is one of the most studied genera of plant growth-promoting rhizobacteria (PGPR). The ability of Azospirillum sp. to promote plant growth has been associated with its ability to produce several phytohormones, such as auxins, gibberellins and cytokinins, but mainly indole-3-acetic acid (IAA). It has been propoosed that the production of IAA explains the positive effects of co-inoculation with Azospirillum sp. on the rhizobia-legume symbiosis. In this study, we constructed an IAA-deficient mutant of A. brasilense Az39 (ipdC−) by using a restriction-free cloning method. We inoculated soybean seeds with 1·106 cfu·seed−1 of
Bradyrhizobium japonicum E109 and co-inoculating leaves at the V3 stage with 1·108 cfu.plant−1 of A. brasilense Az39 wt or ipdC− or inoculated leaves with 20 μg.plant−1 synthetic IAA. The results confirmed soybean growth promotion as there was increased
total plant and root length, aerial and root dry weight, number of nodules on the primary root, and an increase in the symbiosis established with B. japonicum E109. Nodule weight also increased after foliar co-inoculation with the IAA- producer A. brasilense Az39. The exogenous application of IAA decreased aerial and root length, as well as the number of nodules on primary roots in comparison with the Az39 wt strain. These results allow us to propose a biological model of response to foliar co-inoculation of soybean with IAA-producing rhizobacteria. This model clearly shows that both the presence of microorganism as part of the colonization process and the production of IAA in situ are co-responsible, via plant signaling molecules, for the positive effects on plant growth and symbiosis establishment. |
| format | Artículo |
| id | INTA1911 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA19112018-10-03T13:38:17Z The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling model Puente, Mariana Laura Gualpa, Jose Luis López, Gastón A. Molina, Romina M. Carletti, Susana Cassán, Fabricio D. Azospirillum brasilense Inoculación Soja Auxinas Inoculation Soybeans Auxins Inoculación Foliar Azospirillum sp. is one of the most studied genera of plant growth-promoting rhizobacteria (PGPR). The ability of Azospirillum sp. to promote plant growth has been associated with its ability to produce several phytohormones, such as auxins, gibberellins and cytokinins, but mainly indole-3-acetic acid (IAA). It has been propoosed that the production of IAA explains the positive effects of co-inoculation with Azospirillum sp. on the rhizobia-legume symbiosis. In this study, we constructed an IAA-deficient mutant of A. brasilense Az39 (ipdC−) by using a restriction-free cloning method. We inoculated soybean seeds with 1·106 cfu·seed−1 of Bradyrhizobium japonicum E109 and co-inoculating leaves at the V3 stage with 1·108 cfu.plant−1 of A. brasilense Az39 wt or ipdC− or inoculated leaves with 20 μg.plant−1 synthetic IAA. The results confirmed soybean growth promotion as there was increased total plant and root length, aerial and root dry weight, number of nodules on the primary root, and an increase in the symbiosis established with B. japonicum E109. Nodule weight also increased after foliar co-inoculation with the IAA- producer A. brasilense Az39. The exogenous application of IAA decreased aerial and root length, as well as the number of nodules on primary roots in comparison with the Az39 wt strain. These results allow us to propose a biological model of response to foliar co-inoculation of soybean with IAA-producing rhizobacteria. This model clearly shows that both the presence of microorganism as part of the colonization process and the production of IAA in situ are co-responsible, via plant signaling molecules, for the positive effects on plant growth and symbiosis establishment. Inst. de Microbiología y Zoología Agrícola IMyZA Fil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Laboratorio de Bacterias Promotoras del Crecimiento Vegetal; Argentina Fil: Gualpa, Jose Luis. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción planta-microorganismo; Argentina Fil: López, Gastón A. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción planta-microorganismo; Argentina Fil: Molina, Romina M. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción planta-microorganismo; Argentina Fil: Carletti, Susana. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina Fil: Cassán, Fabricio D. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción planta-microorganismo; Argentina 2018-02-28T15:24:37Z 2018-02-28T15:24:37Z 2018-09 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1911 https://link.springer.com/article/10.1007/s13199-017-0536-x 0334-5114 1878-7665 https://doi.org/10.1007/s13199-017-0536-x eng info:eu-repo/semantics/restrictedAccess application/pdf Symbiosis 76 (1) : 41–49 (September 2018) |
| spellingShingle | Azospirillum brasilense Inoculación Soja Auxinas Inoculation Soybeans Auxins Inoculación Foliar Puente, Mariana Laura Gualpa, Jose Luis López, Gastón A. Molina, Romina M. Carletti, Susana Cassán, Fabricio D. The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling model |
| title | The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling model |
| title_full | The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling model |
| title_fullStr | The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling model |
| title_full_unstemmed | The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling model |
| title_short | The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling model |
| title_sort | benefits of foliar inoculation with azospirillum brasilense in soybean are explained by an auxin signaling model |
| topic | Azospirillum brasilense Inoculación Soja Auxinas Inoculation Soybeans Auxins Inoculación Foliar |
| url | http://hdl.handle.net/20.500.12123/1911 https://link.springer.com/article/10.1007/s13199-017-0536-x https://doi.org/10.1007/s13199-017-0536-x |
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