Molecular identification of Sarcocystis aucheniae in the wild South American camelid Vicugna vicugna
Vicuñas (Vicugna vicugna) are wild South American camelids (SACs) protected by law in Argentina, and information on pathogens that infect them is scarce. In this study, an adult vicuña found dead in the province of Salta was examined, and evidence of infection by Sarcocystis sp. protozoans was sough...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2024
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/19074 https://link.springer.com/article/10.1007/s11259-024-10491-0 https://doi.org/10.1007/s11259-024-10491-0 |
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| author | Wieser, Sara Nathaly Cafrune Wierna, María Mercedes Romero, Sandra Raquel Schnittger, Leonhard Florin-Christensen, Monica |
| author_browse | Cafrune Wierna, María Mercedes Florin-Christensen, Monica Romero, Sandra Raquel Schnittger, Leonhard Wieser, Sara Nathaly |
| author_facet | Wieser, Sara Nathaly Cafrune Wierna, María Mercedes Romero, Sandra Raquel Schnittger, Leonhard Florin-Christensen, Monica |
| author_sort | Wieser, Sara Nathaly |
| collection | INTA Digital |
| description | Vicuñas (Vicugna vicugna) are wild South American camelids (SACs) protected by law in Argentina, and information on pathogens that infect them is scarce. In this study, an adult vicuña found dead in the province of Salta was examined, and evidence of infection by Sarcocystis sp. protozoans was sought. Infection of skeletal muscles by S. aucheniae, with the production of macroscopic sarcocysts, a disease known as SAC sarcocystosis, has been described in the other three SACs - llamas, alpacas, and guanacos - but its occurrence in vicuñas has so far remained unknown. In the analyzed individual, many macroscopic cysts compatible with S. aucheniae were found upon necropsy in the muscular tissue of the neck and diaphragm. Analysis of 18 S rRNA and cytochrome c oxidase subunit 1 (cox-1) gene sequences by BLAST searches and construction of phylogenetic trees demonstrated that the etiological agent was S. aucheniae. Our results show for the first time that vicuñas act as intermediate hosts in the life cycle of this parasite. In addition, this study provides the first cox-1 sequences for S. aucheniae isolates from the four SAC species acting as intermediate hosts and suggests that this marker could be useful for genotypification of this parasite species. The impact of SAC sarcocystosis on the health, well-being, and fitness of vicuñas, and the relevance of vicuña infections in the epidemiology of S. auchaniae, remain to be elucidated. |
| format | Artículo |
| id | INTA19074 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA190742024-08-23T10:10:16Z Molecular identification of Sarcocystis aucheniae in the wild South American camelid Vicugna vicugna Wieser, Sara Nathaly Cafrune Wierna, María Mercedes Romero, Sandra Raquel Schnittger, Leonhard Florin-Christensen, Monica Sarcocystosis Camelids Vicugna vicugna Ribosomal RNA Sarcocistosis Camélidos ARN Ribosomial Sarcocystis aucheniae Vicuñas (Vicugna vicugna) are wild South American camelids (SACs) protected by law in Argentina, and information on pathogens that infect them is scarce. In this study, an adult vicuña found dead in the province of Salta was examined, and evidence of infection by Sarcocystis sp. protozoans was sought. Infection of skeletal muscles by S. aucheniae, with the production of macroscopic sarcocysts, a disease known as SAC sarcocystosis, has been described in the other three SACs - llamas, alpacas, and guanacos - but its occurrence in vicuñas has so far remained unknown. In the analyzed individual, many macroscopic cysts compatible with S. aucheniae were found upon necropsy in the muscular tissue of the neck and diaphragm. Analysis of 18 S rRNA and cytochrome c oxidase subunit 1 (cox-1) gene sequences by BLAST searches and construction of phylogenetic trees demonstrated that the etiological agent was S. aucheniae. Our results show for the first time that vicuñas act as intermediate hosts in the life cycle of this parasite. In addition, this study provides the first cox-1 sequences for S. aucheniae isolates from the four SAC species acting as intermediate hosts and suggests that this marker could be useful for genotypification of this parasite species. The impact of SAC sarcocystosis on the health, well-being, and fitness of vicuñas, and the relevance of vicuña infections in the epidemiology of S. auchaniae, remain to be elucidated. Instituto de Patobiología Fil: Wieser, Sarah Nathaly. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Wieser, Sarah Nathaly. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cafrune Wierna, María Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido; Argentina Fil: Romero, Sandra Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación y Desarrollo Tecnológico para la Agricultura Familiar Región NOA; Argentina Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Florin-Christensen, Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-08-23T10:01:11Z 2024-08-23T10:01:11Z 2024-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/19074 https://link.springer.com/article/10.1007/s11259-024-10491-0 1573-7446 https://doi.org/10.1007/s11259-024-10491-0 eng info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Salud info:eu-repograntAgreement/INTA/2019-RIST-E5-I111-001, Laboratorios de Diagnóstico Veterinario info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Veterinary Research Communications : 1-7 (Published: 09 August 2024) |
| spellingShingle | Sarcocystosis Camelids Vicugna vicugna Ribosomal RNA Sarcocistosis Camélidos ARN Ribosomial Sarcocystis aucheniae Wieser, Sara Nathaly Cafrune Wierna, María Mercedes Romero, Sandra Raquel Schnittger, Leonhard Florin-Christensen, Monica Molecular identification of Sarcocystis aucheniae in the wild South American camelid Vicugna vicugna |
| title | Molecular identification of Sarcocystis aucheniae in the wild South American camelid Vicugna vicugna |
| title_full | Molecular identification of Sarcocystis aucheniae in the wild South American camelid Vicugna vicugna |
| title_fullStr | Molecular identification of Sarcocystis aucheniae in the wild South American camelid Vicugna vicugna |
| title_full_unstemmed | Molecular identification of Sarcocystis aucheniae in the wild South American camelid Vicugna vicugna |
| title_short | Molecular identification of Sarcocystis aucheniae in the wild South American camelid Vicugna vicugna |
| title_sort | molecular identification of sarcocystis aucheniae in the wild south american camelid vicugna vicugna |
| topic | Sarcocystosis Camelids Vicugna vicugna Ribosomal RNA Sarcocistosis Camélidos ARN Ribosomial Sarcocystis aucheniae |
| url | http://hdl.handle.net/20.500.12123/19074 https://link.springer.com/article/10.1007/s11259-024-10491-0 https://doi.org/10.1007/s11259-024-10491-0 |
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